BIOMAT Database Logo BIOMAT Database Logo

Isolation and transcriptome analyses of human erythroid progenitors: BFU-E and CFU-E. 2014

Jie Li, and John Hale, and Pooja Bhagia, and Fumin Xue, and Lixiang Chen, and Julie Jaffray, and Hongxia Yan, and Joseph Lane, and Patrick G Gallagher, and Narla Mohandas, and Jing Liu, and Xiuli An
Laboratory of Membrane Biology.

Burst-forming unit-erythroid (BFU-E) and colony-forming unit-erythroid (CFU-E) cells are erythroid progenitors traditionally defined by colony assays. We developed a flow cytometry-based strategy for isolating human BFU-E and CFU-E cells based on the changes in expression of cell surface markers during in vitro erythroid cell culture. BFU-E and CFU-E are characterized by CD45(+)GPA(-)IL-3R(-)CD34(+)CD36(-)CD71(low) and CD45(+)GPA(-)IL-3R(-)CD34(-)CD36(+)CD71(high) phenotypes, respectively. Colony assays validated phenotypic assignment giving rise to BFU-E and CFU-E colonies, both at a purity of ∼90%. The BFU-E colony forming ability of CD45(+)GPA(-)IL-3R(-)CD34(+)CD36(-)CD71(low) cells required stem cell factor and erythropoietin, while the CFU-E colony forming ability of CD45(+)GPA(-)IL-3R(-)CD34(-)CD36(+)CD71(high) cells required only erythropoietin. Bioinformatic analysis of the RNA-sequencing data revealed unique transcriptomes at each differentiation stage. The sorting strategy was validated in uncultured primary cells isolated from bone marrow, cord blood, and peripheral blood, indicating that marker expression is not an artifact of in vitro cell culture, but represents an in vivo characteristic of erythroid progenitor populations. The ability to isolate highly pure human BFU-E and CFU-E progenitors will enable detailed cellular and molecular characterization of these distinct progenitor populations and define their contribution to disordered erythropoiesis in inherited and acquired hematologic disease. Our data provides an important resource for future studies of human erythropoiesis.

UI MeSH Term Description Entries
D008297 Male Males
D002469 Cell Separation Techniques for separating distinct populations of cells. Cell Isolation,Cell Segregation,Isolation, Cell,Cell Isolations,Cell Segregations,Cell Separations,Isolations, Cell,Segregation, Cell,Segregations, Cell,Separation, Cell,Separations, Cell
D004920 Erythropoiesis The production of red blood cells (ERYTHROCYTES). In humans, erythrocytes are produced by the YOLK SAC in the first trimester; by the liver in the second trimester; by the BONE MARROW in the third trimester and after birth. In normal individuals, the erythrocyte count in the peripheral blood remains relatively constant implying a balance between the rate of erythrocyte production and rate of destruction. Erythropoieses
D004921 Erythropoietin Glycoprotein hormone, secreted chiefly by the KIDNEY in the adult and the LIVER in the FETUS, that acts on erythroid stem cells of the BONE MARROW to stimulate proliferation and differentiation.
D005260 Female Females
D005786 Gene Expression Regulation Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation. Gene Action Regulation,Regulation of Gene Expression,Expression Regulation, Gene,Regulation, Gene Action,Regulation, Gene Expression
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000943 Antigens, Differentiation Antigens expressed primarily on the membranes of living cells during sequential stages of maturation and differentiation. As immunologic markers they have high organ and tissue specificity and are useful as probes in studies of normal cell development as well as neoplastic transformation. Differentiation Antigen,Differentiation Antigens,Differentiation Antigens, Hairy Cell Leukemia,Differentiation Marker,Differentiation Markers,Leu Antigen,Leu Antigens,Marker Antigen,Marker Antigens,Markers, Differentiation,Antigen, Differentiation,Antigen, Leu,Antigen, Marker,Antigens, Leu,Antigens, Marker,Marker, Differentiation
D015672 Erythroid Precursor Cells The cells in the erythroid series derived from MYELOID PROGENITOR CELLS or from the bi-potential MEGAKARYOCYTE-ERYTHROID PROGENITOR CELLS which eventually give rise to mature RED BLOOD CELLS. The erythroid progenitor cells develop in two phases: erythroid burst-forming units (BFU-E) followed by erythroid colony-forming units (CFU-E); BFU-E differentiate into CFU-E on stimulation by ERYTHROPOIETIN, and then further differentiate into ERYTHROBLASTS when stimulated by other factors. Burst-Forming Units, Erythroid,Colony-Forming Units, Erythroid,Erythroid Progenitor Cells,Erythropoietic Progenitor Cells,Erythropoietic Stem Cells,Progenitor Cells, Erythropoietic,Stem Cells, Erythroid,BFU-E,CFU-E,BFU E,BFU-Es,Burst Forming Units, Erythroid,Burst-Forming Unit, Erythroid,CFU E,CFU-Es,Cell, Erythroid Precursor,Cell, Erythroid Progenitor,Cell, Erythroid Stem,Cell, Erythropoietic Progenitor,Cell, Erythropoietic Stem,Cells, Erythroid Precursor,Cells, Erythroid Progenitor,Cells, Erythroid Stem,Cells, Erythropoietic Progenitor,Cells, Erythropoietic Stem,Colony Forming Units, Erythroid,Colony-Forming Unit, Erythroid,Erythroid Burst-Forming Unit,Erythroid Burst-Forming Units,Erythroid Colony-Forming Unit,Erythroid Colony-Forming Units,Erythroid Precursor Cell,Erythroid Progenitor Cell,Erythroid Stem Cell,Erythroid Stem Cells,Erythropoietic Progenitor Cell,Erythropoietic Stem Cell,Precursor Cell, Erythroid,Precursor Cells, Erythroid,Progenitor Cell, Erythroid,Progenitor Cell, Erythropoietic,Progenitor Cells, Erythroid,Stem Cell, Erythroid,Stem Cell, Erythropoietic,Stem Cells, Erythropoietic,Unit, Erythroid Burst-Forming,Unit, Erythroid Colony-Forming,Units, Erythroid Burst-Forming,Units, Erythroid Colony-Forming
D059467 Transcriptome The pattern of GENE EXPRESSION at the level of genetic transcription in a specific organism or under specific circumstances in specific cells. Transcriptomes,Gene Expression Profiles,Gene Expression Signatures,Transcriptome Profiles,Expression Profile, Gene,Expression Profiles, Gene,Expression Signature, Gene,Expression Signatures, Gene,Gene Expression Profile,Gene Expression Signature,Profile, Gene Expression,Profile, Transcriptome,Profiles, Gene Expression,Profiles, Transcriptome,Signature, Gene Expression,Signatures, Gene Expression,Transcriptome Profile

Related Publications

Jie Li, and John Hale, and Pooja Bhagia, and Fumin Xue, and Lixiang Chen, and Julie Jaffray, and Hongxia Yan, and Joseph Lane, and Patrick G Gallagher, and Narla Mohandas, and Jing Liu, and Xiuli An
Erythroid progenitors (BFU-e and CFU-e) in acute leukaemia.
July 1979, Journal of clinical pathology,
Jie Li, and John Hale, and Pooja Bhagia, and Fumin Xue, and Lixiang Chen, and Julie Jaffray, and Hongxia Yan, and Joseph Lane, and Patrick G Gallagher, and Narla Mohandas, and Jing Liu, and Xiuli An
Blood erythroid progenitors (CFU-E and BFU-E) in acute lymphoblastic leukemias.
February 1989, Blut,
Jie Li, and John Hale, and Pooja Bhagia, and Fumin Xue, and Lixiang Chen, and Julie Jaffray, and Hongxia Yan, and Joseph Lane, and Patrick G Gallagher, and Narla Mohandas, and Jing Liu, and Xiuli An
Effects of ubenimex on erythroid progenitors (CFU-E and BFU-E) in human bone marrow.
October 1991, Experimental hematology,
Jie Li, and John Hale, and Pooja Bhagia, and Fumin Xue, and Lixiang Chen, and Julie Jaffray, and Hongxia Yan, and Joseph Lane, and Patrick G Gallagher, and Narla Mohandas, and Jing Liu, and Xiuli An
Collagen gel culture of the human hematopoietic progenitors CFU-GM, CFU-E, and BFU-E.
January 1997, Methods in molecular biology (Clifton, N.J.),
Jie Li, and John Hale, and Pooja Bhagia, and Fumin Xue, and Lixiang Chen, and Julie Jaffray, and Hongxia Yan, and Joseph Lane, and Patrick G Gallagher, and Narla Mohandas, and Jing Liu, and Xiuli An
Hemoglobin synthesis in human BFU-E and CFU-E-derived erythroid colonies.
October 1979, Blood,
Jie Li, and John Hale, and Pooja Bhagia, and Fumin Xue, and Lixiang Chen, and Julie Jaffray, and Hongxia Yan, and Joseph Lane, and Patrick G Gallagher, and Narla Mohandas, and Jing Liu, and Xiuli An
In vivo erythropoietin requirements of regenerating erythroid progenitors (BFU-e, CFU-e) in bone marrow of mice.
June 1979, Blood,
Jie Li, and John Hale, and Pooja Bhagia, and Fumin Xue, and Lixiang Chen, and Julie Jaffray, and Hongxia Yan, and Joseph Lane, and Patrick G Gallagher, and Narla Mohandas, and Jing Liu, and Xiuli An
Generation of committed erythroid BFU-E and CFU-E progenitors does not require erythropoietin or the erythropoietin receptor.
October 1995, Cell,
Jie Li, and John Hale, and Pooja Bhagia, and Fumin Xue, and Lixiang Chen, and Julie Jaffray, and Hongxia Yan, and Joseph Lane, and Patrick G Gallagher, and Narla Mohandas, and Jing Liu, and Xiuli An
[Effects of interferon on erythroid progenitor cells (CFU-E, BFU-E)].
May 1986, Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society,
Jie Li, and John Hale, and Pooja Bhagia, and Fumin Xue, and Lixiang Chen, and Julie Jaffray, and Hongxia Yan, and Joseph Lane, and Patrick G Gallagher, and Narla Mohandas, and Jing Liu, and Xiuli An
Responsiveness of bone marrow erythroid progenitors (CFU-E and BFU-E) to recombinant human erythropoietin (rh-Ep) in vitro in multiple myeloma.
August 1992, British journal of haematology,
Jie Li, and John Hale, and Pooja Bhagia, and Fumin Xue, and Lixiang Chen, and Julie Jaffray, and Hongxia Yan, and Joseph Lane, and Patrick G Gallagher, and Narla Mohandas, and Jing Liu, and Xiuli An
Responsiveness to recombinant human erythropoietin (rh-Epo) of marrow erythroid progenitors (CFU-E and BFU-E) from B-chronic lymphocytic leukemia (B-CLL).
June 1997, Journal of experimental & clinical cancer research : CR,
Copied contents to your clipboard!