The purpose of this study was to determine the effect of endpoint knowledge on psychophysiological variables. Twenty-two runners (11 men and 11 women) participated in 2 conditions: a run with an unknown endpoint and a run to the same distance with knowledge of the endpoint. Rating of perceived exertion (RPE), affect, heart rate, and attentional focus were assessed during testing. Subjects ran faster when the endpoint was known (p < 0.01) but no differences in RPE, affect, or heart rate between conditions were present (p > 0.05). There were differences in attentional focus between conditions (p = 0.034) and subjects reported more associative thoughts in the known endpoint condition. Cardiorespiratory fitness was a significant predictor of attentional focus in the known endpoint condition. In sum, when the endpoint was known, subjects used more associative strategies as RPE increased, and those with higher fitness levels used more associative strategies overall.