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Proteinase inhibitor genes are expressed strongly in specific plant tissues under both developmental and environmental regulation. We have studied the role of the 3' control region of the potato proteinase inhibitor II gene (PI-II) that is inducible in leaves in response to herbivore attacks or other severe wounding. Comparison of the terminator from the PI-II gene with two different terminators from the 6b and 7 genes, driven by a common PI-II promoter-cat fusion molecule, indicated that the PI-II terminator provided the most efficient expression of cat. The PI-II terminator also caused a significantly elevated cat gene expression driven by the cauliflower mosaic virus 35S promoter. The increase in the level of expression is probably not due to the presence of an enhancer element in the PI-II terminator region, but to cis-acting elements involved in mRNA processing or stability. Both transient and stable transformation analyses of the deletion mutants in the 3'-flanking sequence indicated that about a 100-base pair DNA fragment surrounding the polyadenylation site is essential for the efficient gene expression. This region seems to consist of several regulatory elements, including the conserved sequence, CGTGTCTT, which is located 9 bases downstream from the polyadenylation site. The elements appear to contribute to the increased stability of mRNAs containing the PI-II terminator.
G An, and
A Mitra, and
H K Choi, and
M A Costa, and
K An, and
R W Thornburg, and
C A Ryan
January 1990,
Proceedings of the National Academy of Sciences of the United States of America,
G An, and
A Mitra, and
H K Choi, and
M A Costa, and
K An, and
R W Thornburg, and
C A Ryan
July 1991,
Planta,
G An, and
A Mitra, and
H K Choi, and
M A Costa, and
K An, and
R W Thornburg, and
C A Ryan
September 1992,
Plant physiology,
G An, and
A Mitra, and
H K Choi, and
M A Costa, and
K An, and
R W Thornburg, and
C A Ryan
February 1987,
The EMBO journal,
G An, and
A Mitra, and
H K Choi, and
M A Costa, and
K An, and
R W Thornburg, and
C A Ryan
January 1994,
Biochemical Society symposium,
G An, and
A Mitra, and
H K Choi, and
M A Costa, and
K An, and
R W Thornburg, and
C A Ryan
January 1990,
The Plant cell,
G An, and
A Mitra, and
H K Choi, and
M A Costa, and
K An, and
R W Thornburg, and
C A Ryan
May 1995,
Proceedings of the National Academy of Sciences of the United States of America,
G An, and
A Mitra, and
H K Choi, and
M A Costa, and
K An, and
R W Thornburg, and
C A Ryan
May 1989,
The EMBO journal,
G An, and
A Mitra, and
H K Choi, and
M A Costa, and
K An, and
R W Thornburg, and
C A Ryan
April 1990,
Plant molecular biology,
G An, and
A Mitra, and
H K Choi, and
M A Costa, and
K An, and
R W Thornburg, and
C A Ryan
September 1990,
Proceedings of the National Academy of Sciences of the United States of America,
