Determination of multiple phytohormones in fruits by high-performance liquid chromatography with fluorescence detection using dispersive liquid-liquid microextraction followed by precolumn fluorescent labeling. 2015

Guoliang Li, and Shuaimin Lu, and Hongliang Wu, and Guang Chen, and Shucheng Liu, and Xiaojian Kong, and Weiheng Kong, and Jinmao You
Key Laboratory of Life-Organic Analysis of Shandong Province, Qufu Normal University, Qufu, P. R. China; College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Zhanjiang, P. R. China.

Plant hormone determination in food matrices has attracted more and more attention because of their potential risks to human health. However, analytical methods for the analysis of multiple plant hormones remain poorly investigated. In the present study, a convenient, selective, and ultrasensitive high-performance liquid chromatography method for the simultaneous determination of multiple classes of plant hormones has been developed successfully using dispersive liquid-liquid microextraction followed by precolumn fluorescent labeling. Eight plant hormones in fruits including jasmonic acid, 12-oxo-phytodienoic acid, indole-3-acetic acid, 3-indolybutyric acid, 3-indolepropionic acid, gibberellin A3 , 1-naphthylacetic acid, and 2-naphthaleneacetic acid were analyzed by this method. The conditions employed for dispersive liquid-liquid microextraction were optimized systematically. The linearity for all plant hormones was found to be >0.9993 (R(2) values). This method offered low detection limits of 0.19-0.44 ng/mL (at a signal-to-noise ratio of 3), and method accuracies were in the range of 92.32-103.10%. The proposed method was applied to determine plant hormones in five kinds of food samples, and this method can achieve a short analysis time, low threshold levels of detection, and a high specificity for the analysis of targeted plant hormones present at trace level concentrations in complex matrices.

UI MeSH Term Description Entries
D009994 Osmolar Concentration The concentration of osmotically active particles in solution expressed in terms of osmoles of solute per liter of solution. Osmolality is expressed in terms of osmoles of solute per kilogram of solvent. Ionic Strength,Osmolality,Osmolarity,Concentration, Osmolar,Concentrations, Osmolar,Ionic Strengths,Osmolalities,Osmolar Concentrations,Osmolarities,Strength, Ionic,Strengths, Ionic
D010937 Plant Growth Regulators Any of the hormones produced naturally in plants and active in controlling growth and other functions. There are three primary classes: auxins, cytokinins, and gibberellins. Phytohormone,Phytohormones,Plant Growth Regulator,Plant Hormone,Plant Hormones,Growth Regulators, Plant,Regulators, Plant Growth,Growth Regulator, Plant,Hormone, Plant,Hormones, Plant,Regulator, Plant Growth
D012015 Reference Standards A basis of value established for the measure of quantity, weight, extent or quality, e.g. weight standards, standard solutions, methods, techniques, and procedures used in diagnosis and therapy. Standard Preparations,Standards, Reference,Preparations, Standard,Standardization,Standards,Preparation, Standard,Reference Standard,Standard Preparation,Standard, Reference
D002851 Chromatography, High Pressure Liquid Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D005456 Fluorescent Dyes Chemicals that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags. Flourescent Agent,Fluorescent Dye,Fluorescent Probe,Fluorescent Probes,Fluorochrome,Fluorochromes,Fluorogenic Substrates,Fluorescence Agents,Fluorescent Agents,Fluorogenic Substrate,Agents, Fluorescence,Agents, Fluorescent,Dyes, Fluorescent,Probes, Fluorescent,Substrates, Fluorogenic
D005638 Fruit The fleshy or dry ripened ovary of a plant, enclosing the seed or seeds. Berries,Legume Pod,Plant Aril,Plant Capsule,Aril, Plant,Arils, Plant,Berry,Capsule, Plant,Capsules, Plant,Fruits,Legume Pods,Plant Arils,Plant Capsules,Pod, Legume,Pods, Legume
D006863 Hydrogen-Ion Concentration The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations
D012997 Solvents Liquids that dissolve other substances (solutes), generally solids, without any change in chemical composition, as, water containing sugar. (Grant & Hackh's Chemical Dictionary, 5th ed) Solvent
D013050 Spectrometry, Fluorescence Measurement of the intensity and quality of fluorescence. Fluorescence Spectrophotometry,Fluorescence Spectroscopy,Spectrofluorometry,Fluorescence Spectrometry,Spectrophotometry, Fluorescence,Spectroscopy, Fluorescence
D013058 Mass Spectrometry An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers. Mass Spectroscopy,Spectrometry, Mass,Spectroscopy, Mass,Spectrum Analysis, Mass,Analysis, Mass Spectrum,Mass Spectrum Analysis,Analyses, Mass Spectrum,Mass Spectrum Analyses,Spectrum Analyses, Mass

Related Publications

Guoliang Li, and Shuaimin Lu, and Hongliang Wu, and Guang Chen, and Shucheng Liu, and Xiaojian Kong, and Weiheng Kong, and Jinmao You
March 2010, Journal of agricultural and food chemistry,
Guoliang Li, and Shuaimin Lu, and Hongliang Wu, and Guang Chen, and Shucheng Liu, and Xiaojian Kong, and Weiheng Kong, and Jinmao You
December 1978, Analytical biochemistry,
Guoliang Li, and Shuaimin Lu, and Hongliang Wu, and Guang Chen, and Shucheng Liu, and Xiaojian Kong, and Weiheng Kong, and Jinmao You
September 2015, Journal of separation science,
Guoliang Li, and Shuaimin Lu, and Hongliang Wu, and Guang Chen, and Shucheng Liu, and Xiaojian Kong, and Weiheng Kong, and Jinmao You
April 2010, Analytical and bioanalytical chemistry,
Guoliang Li, and Shuaimin Lu, and Hongliang Wu, and Guang Chen, and Shucheng Liu, and Xiaojian Kong, and Weiheng Kong, and Jinmao You
November 2013, Journal of pharmaceutical and biomedical analysis,
Guoliang Li, and Shuaimin Lu, and Hongliang Wu, and Guang Chen, and Shucheng Liu, and Xiaojian Kong, and Weiheng Kong, and Jinmao You
May 1987, Journal of pharmacological methods,
Guoliang Li, and Shuaimin Lu, and Hongliang Wu, and Guang Chen, and Shucheng Liu, and Xiaojian Kong, and Weiheng Kong, and Jinmao You
October 2011, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,
Guoliang Li, and Shuaimin Lu, and Hongliang Wu, and Guang Chen, and Shucheng Liu, and Xiaojian Kong, and Weiheng Kong, and Jinmao You
January 2014, Iranian journal of pharmaceutical research : IJPR,
Guoliang Li, and Shuaimin Lu, and Hongliang Wu, and Guang Chen, and Shucheng Liu, and Xiaojian Kong, and Weiheng Kong, and Jinmao You
June 2012, Se pu = Chinese journal of chromatography,
Guoliang Li, and Shuaimin Lu, and Hongliang Wu, and Guang Chen, and Shucheng Liu, and Xiaojian Kong, and Weiheng Kong, and Jinmao You
September 2012, Journal of separation science,
Copied contents to your clipboard!