Biomaterial Database

Increased cell proliferation and differential protein expression induced by low-level Er:YAG laser irradiation in human gingival fibroblasts: proteomic analysis. 2015

Mayumi Ogita, and Sachio Tsuchida, and Akira Aoki, and Mamoru Satoh, and Sayaka Kado, and Masanori Sawabe, and Hiromi Nanbara, and Hiroaki Kobayashi, and Yasuo Takeuchi, and Koji Mizutani, and Yoshiyuki Sasaki, and Fumio Nomura, and Yuichi Izumi

Department of Periodontology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU), 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8549, Japan.

Erbium-doped yttrium aluminum garnet (Er:YAG) laser treatment has demonstrated favorable wound healing effect after periodontal therapy. One of the reasons may be the positive biological effect of the low-level laser on the irradiated tissues, although the mechanism remains unclear. The aim of this study was to investigate the effect of low-level Er:YAG laser irradiation on cell proliferation and laser-induced differential expression of proteins in human gingival fibroblasts (HGFs) by proteomic analysis. In the first experiment, HGFs were exposed to low-level Er:YAG laser irradiation and the laser-induced cell proliferation and damage were evaluated on day 3. In the second experiment, proteomic analysis was performed on day 1 after irradiation. The peptides prepared from HGFs were analyzed by a hybrid ion trap-Fourier transform mass spectrometer, Mascot search engine, and UniProtKB database. A significant increase in cell proliferation without cell damage after irradiation was observed. Among the total identified 377 proteins, 59 proteins, including galectin-7, which was associated with the process of wound healing, were upregulated and 15 proteins were downregulated in laser-treated HGFs. In the third experiment, the increase in messenger RNA (mRNA) and protein expression of galectin-7 in the irradiated HGFs was validated by various analytical techniques. In addition, the effect of recombinant human galectin-7 on the modulation of HGFs proliferation was confirmed. The results indicate that low-level Er:YAG laser irradiation can promote HGF proliferation and induce a significant change in protein expression and the upregulation of galectin-7 expression may partly contribute to the increase in cell proliferation.

UI	MeSH Term	Description	Entries
D010518	Periodontitis	Inflammation and loss of connective tissues supporting or surrounding the teeth. This may involve any part of the PERIODONTIUM. Periodontitis is currently classified by disease progression (CHRONIC PERIODONTITIS; AGGRESSIVE PERIODONTITIS) instead of age of onset. (From 1999 International Workshop for a Classification of Periodontal Diseases and Conditions, American Academy of Periodontology)	Pericementitis,Pericementitides,Periodontitides
D002478	Cells, Cultured	Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.	Cultured Cells,Cell, Cultured,Cultured Cell
D005347	Fibroblasts	Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.	Fibroblast
D005881	Gingiva	Oral tissue surrounding and attached to TEETH.	Gums,Interdental Papilla,Papilla, Interdental,Gum
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D015870	Gene Expression	The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.	Expression, Gene,Expressions, Gene,Gene Expressions
D049109	Cell Proliferation	All of the processes involved in increasing CELL NUMBER including CELL DIVISION.	Cell Growth in Number,Cellular Proliferation,Cell Multiplication,Cell Number Growth,Growth, Cell Number,Multiplication, Cell,Number Growth, Cell,Proliferation, Cell,Proliferation, Cellular
D053844	Lasers, Solid-State	Lasers which use a solid, as opposed to a liquid or gas, as the lasing medium. Common materials used are crystals, such as YAG (YTTRIUM aluminum garnet); alexandrite; and CORUNDUM, doped with a rare earth element such as a NEODYMIUM; ERBIUM; or HOLMIUM. The output is sometimes additionally modified by addition of non-linear optical materials such as potassium titanyl phosphate crystal, which for example is used with neodymium YAG lasers to convert the output light to the visible range.	Alexandrite Laser,Alexandrite Lasers,Diode Pumped Solid State Laser,Diode Pumped Solid State Lasers,Er-YAG Laser,Er-YAG Lasers,Erbium Doped Yttrium Aluminum Garnet Laser,Erbium YAG Laser,Erbium-Doped Yttrium Aluminum Garnet Laser,Erbium-Doped Yttrium Aluminum Garnet Lasers,Ho YAG Laser,Ho YAG Lasers,Holmium Doped Yttrium Aluminum Garnet Lasers,Holmium Laser,Holmium-YAG Laser,Holmium-YAG Lasers,KTP Laser,Laser, Nd-YAG,Nd-YAG Laser,Nd-YAG Lasers,Neodymium-Doped Yttrium Aluminum Garnet Laser,Neodymium-Doped Yttrium Aluminum Garnet Lasers,Potassium Titanyl Phosphate Laser,Ruby Laser,Ruby Lasers,Solid-State Laser,YAG Laser,YAG Lasers,YLF Laser,YLF Lasers,YSGG Laser,YSGG Lasers,Yttrium Aluminum Garnet Laser,Yttrium-Lithium-Fluoride Laser,Yttrium-Lithium-Fluoride Lasers,Yttrium-Scandium-Gallium Garnet Laser,Yttrium-Scandium-Gallium Garnet Lasers,Erbium YAG Lasers,Holmium Lasers,KTP Lasers,Lasers, Alexandrite,Lasers, Diode Pumped Solid State,Lasers, Er-YAG,Lasers, Erbium-Doped Yttrium Aluminum Garnet,Lasers, Ho-YAG,Lasers, Holmium Doped Yttrium Aluminum Garnet,Lasers, Nd-YAG,Lasers, Neodymium-Doped Yttrium Aluminum Garnet,Lasers, Ruby,Lasers, YAG,Lasers, Yttrium Aluminum Garnet,Lasers, Yttrium-Lithium-Fluoride,Potassium Titanyl Phosphate Lasers,Yttrium Aluminum Garnet Lasers,Er YAG Laser,Er YAG Lasers,Erbium Doped Yttrium Aluminum Garnet Lasers,Ho-YAG Laser,Ho-YAG Lasers,Holmium YAG Laser,Holmium YAG Lasers,Laser, Alexandrite,Laser, Er-YAG,Laser, Erbium YAG,Laser, Ho YAG,Laser, Ho-YAG,Laser, Holmium,Laser, Holmium-YAG,Laser, KTP,Laser, Nd YAG,Laser, Ruby,Laser, Solid-State,Laser, YAG,Laser, YLF,Laser, YSGG,Laser, Yttrium-Lithium-Fluoride,Laser, Yttrium-Scandium-Gallium Garnet,Lasers, Er YAG,Lasers, Erbium Doped Yttrium Aluminum Garnet,Lasers, Erbium YAG,Lasers, Ho YAG,Lasers, Holmium,Lasers, Holmium-YAG,Lasers, KTP,Lasers, Nd YAG,Lasers, Neodymium Doped Yttrium Aluminum Garnet,Lasers, Solid State,Lasers, YLF,Lasers, YSGG,Lasers, Yttrium Lithium Fluoride,Lasers, Yttrium-Scandium-Gallium Garnet,Nd YAG Laser,Nd YAG Lasers,Neodymium Doped Yttrium Aluminum Garnet Laser,Neodymium Doped Yttrium Aluminum Garnet Lasers,Solid State Laser,Solid-State Lasers,YAG Laser, Erbium,YAG Laser, Ho,YAG Lasers, Erbium,YAG Lasers, Ho,Yttrium Lithium Fluoride Laser,Yttrium Lithium Fluoride Lasers,Yttrium Scandium Gallium Garnet Laser,Yttrium Scandium Gallium Garnet Lasers
D020543	Proteome	The protein complement of an organism coded for by its genome.	Proteomes
D028022	Low-Level Light Therapy	Treatment using irradiation with light of low power intensity so that the effects are a response to the light and not due to heat. A variety of light sources, especially low-power lasers are used.	LLLT,Laser Biostimulation,Laser Irradiation, Low-Power,Laser Therapy, Low-Level,Photobiomodulation,Laser Phototherapy,Laser Therapy, Low-Power,Low-Level Laser Therapy,Low-Power Laser Irradiation,Low-Power Laser Therapy,Photobiomodulation Therapy,Biostimulation, Laser,Irradiation, Low-Power Laser,Laser Irradiation, Low Power,Laser Therapies, Low-Level,Laser Therapies, Low-Power,Laser Therapy, Low Level,Laser Therapy, Low Power,Light Therapies, Low-Level,Light Therapy, Low-Level,Low Level Laser Therapy,Low Level Light Therapy,Low Power Laser Irradiation,Low Power Laser Therapy,Low-Level Laser Therapies,Low-Level Light Therapies,Low-Power Laser Therapies,Photobiomodulation Therapies,Photobiomodulations,Phototherapy, Laser,Therapies, Low-Level Light,Therapies, Photobiomodulation,Therapy, Low-Level Light,Therapy, Photobiomodulation