-transparent.png){kind=logo}
The kinetics of inhibition of herpes simplex virus type 1 (HSV 1) on both diploid (CEF) and heteroploid cells (HEp2) by light-irradiated haematoporphyrin (HP) was studied. The inactivation of HSV1 by HP was drug-dose dependent and light-irradiation dependent; the viruses grown in heteroploid cells being in all cases more sensitive to inhibition than viruses grown in diploid cells. Cell toxicity by HP was markedly more evident on HEp2 cells than on CEF. The highest viral sensitivity to photodynamic inactivation by HP was found to be between the 4th and the 5th hour after cell infection, when the viral DNA synthesis is at its peak and before it is incorporated into complete virions. Microfluorometric and spectrofluorometric assays revealed that virus infected cells always take up more HP than uninfected cells, and heteroploid cells incorporated more HP than diploid cells. The possibility that an increased uptake of HP and modifications of the cell micro-environment in virus infected cells could account for the viral-inhibiting properties of HP, is discussed.
R Pompei, and
G Cisani, and
G Foddis, and
M A Marcialis
November 1963,
Journal of immunology (Baltimore, Md. : 1950),
R Pompei, and
G Cisani, and
G Foddis, and
M A Marcialis
September 1965,
Life sciences,
R Pompei, and
G Cisani, and
G Foddis, and
M A Marcialis
December 1979,
Biotechnology and bioengineering,
R Pompei, and
G Cisani, and
G Foddis, and
M A Marcialis
March 1965,
Virology,
R Pompei, and
G Cisani, and
G Foddis, and
M A Marcialis
January 1992,
Anticancer research,
R Pompei, and
G Cisani, and
G Foddis, and
M A Marcialis
February 1984,
Journal of virology,
R Pompei, and
G Cisani, and
G Foddis, and
M A Marcialis
July 1967,
Acta virologica,
R Pompei, and
G Cisani, and
G Foddis, and
M A Marcialis
March 1969,
Virology,
R Pompei, and
G Cisani, and
G Foddis, and
M A Marcialis
June 1982,
Investigative ophthalmology & visual science,
R Pompei, and
G Cisani, and
G Foddis, and
M A Marcialis
January 1988,
Archives of virology,
