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Specific receptors for glucocorticoids were identified in the fetal and adult sheep adrenal cortex by a whole-cell binding assay using [3H]triamcinolone acetonide ([3H]TA) as the radiolabelled ligand. [3H]TA binding sites were saturable and of high affinity, with dissociation constant (Kd) of 2-3nM. Scatchard analysis revealed a single class of binding sites with a binding capacity (Bmax) of 207 and 5 fmol/10(6) cells for d100 fetuses and adults, respectively. By single point analyses at saturating [3H]TA concentration, we found that glucocorticoid receptors (GR) were present in the fetal adrenal cortex as early as d60. Highest concentrations were found at d100-110. GR decreased to d125, then increased to term (approx. d145) before declining again in newborn and adult animals. This demonstration of glucocorticoid receptors in ovine fetal adrenal cortical cells provides a mechanistic basis for the concept that glucocorticoids may act, perhaps in a paracrine or autocrine fashion, to influence adrenocorticotropin (ACTH)-induced activation of fetal adrenal function and the events leading to parturition.
K Yang, and
J R Challis
July 1980,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
K Yang, and
J R Challis
April 1974,
The Journal of biological chemistry,
K Yang, and
J R Challis
July 1990,
The Journal of endocrinology,
K Yang, and
J R Challis
December 1987,
Molecular and cellular biology,
K Yang, and
J R Challis
January 1988,
Biochemical and biophysical research communications,
K Yang, and
J R Challis
April 1994,
Journal of neuroendocrinology,
K Yang, and
J R Challis
December 1992,
American journal of obstetrics and gynecology,
K Yang, and
J R Challis
April 1967,
Acta physiologica Scandinavica,
K Yang, and
J R Challis
February 1994,
Clinical endocrinology,
