BIOMAT Database Logo BIOMAT Database Logo

Resistance of Trichoplusia ni populations selected by Bacillus thuringiensis sprays to cotton plants expressing pyramided Bacillus thuringiensis toxins Cry1Ac and Cry2Ab. 2015

Wendy Kain, and Xiaozhao Song, and Alida F Janmaat, and Jian-Zhou Zhao, and Judith Myers, and Anthony M Shelton, and Ping Wang
Department of Entomology, Cornell University, New York State Agricultural Experiment Station, Geneva, New York, USA.

Two populations of Trichoplusia ni that had developed resistance to Bacillus thuringiensis sprays (Bt sprays) in commercial greenhouse vegetable production were tested for resistance to Bt cotton (BollGard II) plants expressing pyramided Cry1Ac and Cry2Ab. The T. ni colonies resistant to Bacillus thuringiensis serovar kurstaki formulations were not only resistant to the Bt toxin Cry1Ac, as previously reported, but also had a high frequency of Cry2Ab-resistant alleles, exhibiting ca. 20% survival on BollGard II foliage. BollGard II-resistant T. ni strains were established by selection with BollGard II foliage to further remove Cry2Ab-sensitive alleles in the T. ni populations. The BollGard II-resistant strains showed incomplete resistance to BollGard II, with adjusted survival values of 0.50 to 0.78 after 7 days. The resistance to the dual-toxin cotton plants was conferred by two genetically independent resistance mechanisms: one to Cry1Ac and one to Cry2Ab. The 50% lethal concentration of Cry2Ab for the resistant strain was at least 1,467-fold that for the susceptible T. ni strain. The resistance to Cry2Ab in resistant T. ni was an autosomally inherited, incompletely recessive monogenic trait. Results from this study indicate that insect populations under selection by Bt sprays in agriculture can be resistant to multiple Bt toxins and may potentially confer resistance to multitoxin Bt crops.

UI MeSH Term Description Entries
D007915 Lepidoptera A large order of insects comprising the butterflies and moths.
D003368 Gossypium A plant genus of the family MALVACEAE. It is the source of COTTON FIBER; COTTONSEED OIL, which is used for cooking, and GOSSYPOL. The economically important cotton crop is a major user of agricultural PESTICIDES. Cotton Plant,Cotton Plants,Gossypiums,Plant, Cotton,Plants, Cotton
D004351 Drug Resistance Diminished or failed response of an organism, disease or tissue to the intended effectiveness of a chemical or drug. It should be differentiated from DRUG TOLERANCE which is the progressive diminution of the susceptibility of a human or animal to the effects of a drug, as a result of continued administration. Resistance, Drug
D004731 Endotoxins Toxins closely associated with the living cytoplasm or cell wall of certain microorganisms, which do not readily diffuse into the culture medium, but are released upon lysis of the cells. Endotoxin
D006460 Hemolysin Proteins Proteins from BACTERIA and FUNGI that are soluble enough to be secreted to target ERYTHROCYTES and insert into the membrane to form beta-barrel pores. Biosynthesis may be regulated by HEMOLYSIN FACTORS. Hemolysin,Hemolysins,Hemalysins,Proteins, Hemolysin
D000083722 Bacillus thuringiensis Toxins Endotoxins produced by BACILLUS THURINGIENSIS used in transgenic plants and insecticides. When eaten by a susceptible insect they are protease activated in the insect midgut resulting in death from bacterial septicemia. B thuringiensis Toxins,B. thuringiensis Toxins,Bt Toxin,Bt Toxins,Toxin, Bt,Toxins, B thuringiensis,Toxins, B. thuringiensis
D000483 Alleles Variant forms of the same gene, occupying the same locus on homologous CHROMOSOMES, and governing the variants in production of the same gene product. Allelomorphs,Allele,Allelomorph
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001426 Bacterial Proteins Proteins found in any species of bacterium. Bacterial Gene Products,Bacterial Gene Proteins,Gene Products, Bacterial,Bacterial Gene Product,Bacterial Gene Protein,Bacterial Protein,Gene Product, Bacterial,Gene Protein, Bacterial,Gene Proteins, Bacterial,Protein, Bacterial,Proteins, Bacterial
D012641 Selection, Genetic Differential and non-random reproduction of different genotypes, operating to alter the gene frequencies within a population. Natural Selection,Genetic Selection,Selection, Natural

Related Publications

Wendy Kain, and Xiaozhao Song, and Alida F Janmaat, and Jian-Zhou Zhao, and Judith Myers, and Anthony M Shelton, and Ping Wang
Asymmetrical cross-resistance between Bacillus thuringiensis toxins Cry1Ac and Cry2Ab in pink bollworm.
July 2009, Proceedings of the National Academy of Sciences of the United States of America,
Wendy Kain, and Xiaozhao Song, and Alida F Janmaat, and Jian-Zhou Zhao, and Judith Myers, and Anthony M Shelton, and Ping Wang
Modified Bacillus thuringiensis toxins and a hybrid B. thuringiensis strain counter greenhouse-selected resistance in Trichoplusia ni.
September 2009, Applied and environmental microbiology,
Wendy Kain, and Xiaozhao Song, and Alida F Janmaat, and Jian-Zhou Zhao, and Judith Myers, and Anthony M Shelton, and Ping Wang
Resistance to Bacillus thuringiensis toxin Cry2Ab and survival on single-toxin and pyramided cotton in cotton bollworm from China.
February 2017, Evolutionary applications,
Wendy Kain, and Xiaozhao Song, and Alida F Janmaat, and Jian-Zhou Zhao, and Judith Myers, and Anthony M Shelton, and Ping Wang
Effect of crop plants on fitness costs associated with resistance to Bacillus thuringiensis toxins Cry1Ac and Cry2Ab in cabbage loopers.
February 2016, Scientific reports,
Wendy Kain, and Xiaozhao Song, and Alida F Janmaat, and Jian-Zhou Zhao, and Judith Myers, and Anthony M Shelton, and Ping Wang
Effect of Bt cotton expressing Cry1Ac and Cry2Ab, non-Bt cotton and starvation on survival and development of Trichoplusia ni (Lepidoptera: Noctuidae).
May 2007, Pest management science,
Wendy Kain, and Xiaozhao Song, and Alida F Janmaat, and Jian-Zhou Zhao, and Judith Myers, and Anthony M Shelton, and Ping Wang
Resistance of Trichoplusia ni to Bacillus thuringiensis toxin Cry1Ac is independent of alteration of the cadherin-like receptor for Cry toxins.
January 2012, PloS one,
Wendy Kain, and Xiaozhao Song, and Alida F Janmaat, and Jian-Zhou Zhao, and Judith Myers, and Anthony M Shelton, and Ping Wang
[Characterization of resistant cell line of Trichoplusia ni to Bacillus thuringiensis toxin Cry1Ac].
June 2004, Shi yan sheng wu xue bao,
Wendy Kain, and Xiaozhao Song, and Alida F Janmaat, and Jian-Zhou Zhao, and Judith Myers, and Anthony M Shelton, and Ping Wang
Mechanism of resistance to Bacillus thuringiensis toxin Cry1Ac in a greenhouse population of the cabbage looper, Trichoplusia ni.
February 2007, Applied and environmental microbiology,
Wendy Kain, and Xiaozhao Song, and Alida F Janmaat, and Jian-Zhou Zhao, and Judith Myers, and Anthony M Shelton, and Ping Wang
Frequency of alleles conferring resistance to the Bacillus thuringiensis toxins Cry1Ac and Cry2Ab in Australian populations of Helicoverpa punctigera (Lepidoptera: Noctuidae) from 2002 to 2006.
April 2009, Journal of economic entomology,
Wendy Kain, and Xiaozhao Song, and Alida F Janmaat, and Jian-Zhou Zhao, and Judith Myers, and Anthony M Shelton, and Ping Wang
Aminopeptidase dependent pore formation of Bacillus thuringiensis Cry1Ac toxin on Trichoplusia ni membranes.
September 1997, FEBS letters,
Copied contents to your clipboard!