Terminal cisternae of denervated rabbit skeletal muscle: alterations of functional properties of Ca2+ release channels. 1989

F Zorzato, and P Volpe, and E Damiani, and D Quaglino, and A Margreth
Centro di Studio per la Biologia e la Fisiopatologia Muscolare del Consiglio Nazionale delle Ricerche, Istituto di Patologia Generale dell'Università di Padova, Padua, Italy.

Terminal cisternae (TC) of skeletal muscle represent the specialized compartment from which Ca2+ is released into the myoplasm after a propagated action potential. In this study we have investigated the morphology, protein composition, and Ca2+ release properties of TC isolated from rabbit gastrocnemius muscle 2 wk after nerve sectioning. Thin-section electron microscopy showed that TC vesicles from denervated muscle were enriched in calsequestrin (CS) and contained a larger fraction of the junctional sarcoplasmic reticulum (SR), as judged by membrane profiles with morphologically intact feet structures. Accordingly, the yield of junctional SR from denervated muscle was twice that of control muscle, and the protein pattern of TC vesicles exhibited an increase in junctional protein components, e.g., CS and the 350-kDa protein. The larger content of the 350-kDa protein, or ryanodine receptor (F.A. Lai, H. Erickson, E. Rousseau, Q.-Y. Liu, and G. Meissner, Nature Lond. 331: 315-319, 1988; T. Imagawa, J. S. Smith, R. Coronado, and K. P. Campbell. J. Biol. Chem. 262: 10636-10643, 1987; L. Hymel, M. Inui, S. Fleischer, and H. Schindler, Proc. Natl. Acad. Sci. USA 85:441-445, 1988) was paralleled by an increased binding site density (Bmax) for ryanodine binding in denervated muscle TC. The effects of ruthenium red, a Ca2+ release blocker, on Ca2+ loading rate and Ca2+-ATPase activity suggested that TC from denervated muscle were less permeable to Ca2+. After active Ca2+ loading, both doxorubicin and caffeine induced Ca2+ release from isolated TC, yet Ca2+ release rates were reduced in denervated muscle TC.(ABSTRACT TRUNCATED AT 250 WORDS)

UI MeSH Term Description Entries
D009121 Muscle Denervation The resection or removal of the innervation of a muscle or muscle tissue. Denervation, Muscle,Denervations, Muscle,Muscle Denervations
D009132 Muscles Contractile tissue that produces movement in animals. Muscle Tissue,Muscle,Muscle Tissues,Tissue, Muscle,Tissues, Muscle
D011817 Rabbits A burrowing plant-eating mammal with hind limbs that are longer than its fore limbs. It belongs to the family Leporidae of the order Lagomorpha, and in contrast to hares, possesses 22 instead of 24 pairs of chromosomes. Belgian Hare,New Zealand Rabbit,New Zealand Rabbits,New Zealand White Rabbit,Rabbit,Rabbit, Domestic,Chinchilla Rabbits,NZW Rabbits,New Zealand White Rabbits,Oryctolagus cuniculus,Chinchilla Rabbit,Domestic Rabbit,Domestic Rabbits,Hare, Belgian,NZW Rabbit,Rabbit, Chinchilla,Rabbit, NZW,Rabbit, New Zealand,Rabbits, Chinchilla,Rabbits, Domestic,Rabbits, NZW,Rabbits, New Zealand,Zealand Rabbit, New,Zealand Rabbits, New,cuniculus, Oryctolagus
D002110 Caffeine A methylxanthine naturally occurring in some beverages and also used as a pharmacological agent. Caffeine's most notable pharmacological effect is as a central nervous system stimulant, increasing alertness and producing agitation. It also relaxes SMOOTH MUSCLE, stimulates CARDIAC MUSCLE, stimulates DIURESIS, and appears to be useful in the treatment of some types of headache. Several cellular actions of caffeine have been observed, but it is not entirely clear how each contributes to its pharmacological profile. Among the most important are inhibition of cyclic nucleotide PHOSPHODIESTERASES, antagonism of ADENOSINE RECEPTORS, and modulation of intracellular calcium handling. 1,3,7-Trimethylxanthine,Caffedrine,Coffeinum N,Coffeinum Purrum,Dexitac,Durvitan,No Doz,Percoffedrinol N,Percutaféine,Quick-Pep,Vivarin,Quick Pep,QuickPep
D002118 Calcium A basic element found in nearly all tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes. Coagulation Factor IV,Factor IV,Blood Coagulation Factor IV,Calcium-40,Calcium 40,Factor IV, Coagulation
D002458 Cell Fractionation Techniques to partition various components of the cell into SUBCELLULAR FRACTIONS. Cell Fractionations,Fractionation, Cell,Fractionations, Cell
D004317 Doxorubicin Antineoplastic antibiotic obtained from Streptomyces peucetius. It is a hydroxy derivative of DAUNORUBICIN. Adriamycin,Adriablastin,Adriablastine,Adriblastin,Adriblastina,Adriblastine,Adrimedac,DOXO-cell,Doxolem,Doxorubicin Hexal,Doxorubicin Hydrochloride,Doxorubicin NC,Doxorubicina Ferrer Farm,Doxorubicina Funk,Doxorubicina Tedec,Doxorubicine Baxter,Doxotec,Farmiblastina,Myocet,Onkodox,Ribodoxo,Rubex,Urokit Doxo-cell,DOXO cell,Hydrochloride, Doxorubicin,Urokit Doxo cell
D000255 Adenosine Triphosphate An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter. ATP,Adenosine Triphosphate, Calcium Salt,Adenosine Triphosphate, Chromium Salt,Adenosine Triphosphate, Magnesium Salt,Adenosine Triphosphate, Manganese Salt,Adenylpyrophosphate,CaATP,CrATP,Manganese Adenosine Triphosphate,MgATP,MnATP,ATP-MgCl2,Adenosine Triphosphate, Chromium Ammonium Salt,Adenosine Triphosphate, Magnesium Chloride,Atriphos,Chromium Adenosine Triphosphate,Cr(H2O)4 ATP,Magnesium Adenosine Triphosphate,Striadyne,ATP MgCl2
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012519 Sarcoplasmic Reticulum A network of tubules and sacs in the cytoplasm of SKELETAL MUSCLE FIBERS that assist with muscle contraction and relaxation by releasing and storing calcium ions. Reticulum, Sarcoplasmic,Reticulums, Sarcoplasmic,Sarcoplasmic Reticulums

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