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The human promyelocytic leukemia cell line HL-60 can be induced to differentiate into granulocytes upon exposure to retinoids. Previously we have shown that extracts of undifferentiated HL-60 cells possess a specific retinoid-binding activity (RSBP-1) corresponding to an approximate 95 kilodalton (kDa) protein as determined by size-exclusion chromatography. We now extend these observations to reveal a second approximate 95 kDa retinoic acid-binding component (RSBP-2), which is separable from RSBP-1 using anion exchange chromatography. We further show that the chromatographic properties of RSBP-1 and RSBP-2 are identical to those found for the retinoid-binding activities present in extracts of HeLa cells transfected with the human retinoic acid receptor (RAR) expression vectors RAR-beta phi and RAR-alpha phi, respectively. Moreover, an antiserum preparation directed against RAR-beta selectively immunoprecipitated both the retinoid-binding activity in extracts of HeLa cells transfected with RAR-beta phi and that corresponding to RSBP-1 in HL-60 cell extracts. Similarly, an antiserum preparation directed against RAR-alpha immunoprecipitated the retinoid-binding activity in extracts from RAR-alpha phi transfected HeLa cell as well as that corresponding to RSBP-2 in HL-60 cell extracts. Using these antisera, Western blot analyses of extracts from HL-60 cells, and from HeLa cells transfected with either RAR-alpha phi or RAR-beta phi, confirmed that RSBP-2 and RSBP-1 are identical to RAR-alpha and RAR-beta, respectively. However, RAR-alpha, RAR-beta, RSBP-1, and RSBP-2 appeared as an approximate 51 kDa species in sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis in contrast with an apparent approximate 95 k mol wt as estimated from size-exclusion chromatography in the presence of 0.6 M KCl.(ABSTRACT TRUNCATED AT 250 WORDS)
Y Hashimoto, and
M Petkovich, and
M P Gaub, and
H Kagechika, and
K Shudo, and
P Chambon
May 1980,
Proceedings of the National Academy of Sciences of the United States of America,
Y Hashimoto, and
M Petkovich, and
M P Gaub, and
H Kagechika, and
K Shudo, and
P Chambon
September 1989,
Biochemical and biophysical research communications,
Y Hashimoto, and
M Petkovich, and
M P Gaub, and
H Kagechika, and
K Shudo, and
P Chambon
January 1981,
Biochemical and biophysical research communications,
Y Hashimoto, and
M Petkovich, and
M P Gaub, and
H Kagechika, and
K Shudo, and
P Chambon
February 1987,
Blood,
Y Hashimoto, and
M Petkovich, and
M P Gaub, and
H Kagechika, and
K Shudo, and
P Chambon
July 1988,
Journal of leukocyte biology,
Y Hashimoto, and
M Petkovich, and
M P Gaub, and
H Kagechika, and
K Shudo, and
P Chambon
July 1984,
The Journal of experimental medicine,
Y Hashimoto, and
M Petkovich, and
M P Gaub, and
H Kagechika, and
K Shudo, and
P Chambon
September 1999,
The Tohoku journal of experimental medicine,
Y Hashimoto, and
M Petkovich, and
M P Gaub, and
H Kagechika, and
K Shudo, and
P Chambon
January 1993,
Life sciences,
Y Hashimoto, and
M Petkovich, and
M P Gaub, and
H Kagechika, and
K Shudo, and
P Chambon
November 1991,
Biochemical and biophysical research communications,
Y Hashimoto, and
M Petkovich, and
M P Gaub, and
H Kagechika, and
K Shudo, and
P Chambon
April 1985,
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