BIOMAT Database Logo BIOMAT Database Logo

Effects of vanadate on protein kinases in rat hepatocytes. 1989

C Villar-Palasi, and J J Guinovart, and A M Gómez-Foix, and J E Rodriguez-Gil, and F Bosch
Department of Biochemistry, Autonomous University of Barcelona, School of Veterinary Medicine, Spain.

In rat hepatocytes, vanadate modifies neither the intracellular concentration of cyclic AMP nor the --cyclic AMP/+cyclic AMP activity ratio for cyclic AMP-dependent protein kinase. Vanadate can, however, counteract the increase in cyclic AMP and the increase in the --cyclic AMP/+cyclic AMP activity ratio of cyclic AMP-dependent protein kinase induced by glucagon. On the other hand, vanadate treatment of hepatocytes can produce a time- and concentration-dependent increase in cyclic AMP- and Ca2+-independent casein kinase activity. Maximal activation at the optimal time with 5 mM-vanadate was about 70% over control. A clear relationship was observed between the activation of casein kinase and the inactivation of glycogen synthase after vanadate treatment. These results suggest that casein kinase activity may be involved in vanadate actions in rat hepatocytes.

UI MeSH Term Description Entries
D008099 Liver A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances. Livers
D008297 Male Males
D011494 Protein Kinases A family of enzymes that catalyze the conversion of ATP and a protein to ADP and a phosphoprotein. Protein Kinase,Kinase, Protein,Kinases, Protein
D011919 Rats, Inbred Strains Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations or by parent x offspring matings carried out with certain restrictions. This also includes animals with a long history of closed colony breeding. August Rats,Inbred Rat Strains,Inbred Strain of Rat,Inbred Strain of Rats,Inbred Strains of Rats,Rat, Inbred Strain,August Rat,Inbred Rat Strain,Inbred Strain Rat,Inbred Strain Rats,Inbred Strains Rat,Inbred Strains Rats,Rat Inbred Strain,Rat Inbred Strains,Rat Strain, Inbred,Rat Strains, Inbred,Rat, August,Rat, Inbred Strains,Rats Inbred Strain,Rats Inbred Strains,Rats, August,Rats, Inbred Strain,Strain Rat, Inbred,Strain Rats, Inbred,Strain, Inbred Rat,Strains, Inbred Rat
D002118 Calcium A basic element found in nearly all tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes. Coagulation Factor IV,Factor IV,Blood Coagulation Factor IV,Calcium-40,Calcium 40,Factor IV, Coagulation
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D004789 Enzyme Activation Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme. Activation, Enzyme,Activations, Enzyme,Enzyme Activations
D000242 Cyclic AMP An adenine nucleotide containing one phosphate group which is esterified to both the 3'- and 5'-positions of the sugar moiety. It is a second messenger and a key intracellular regulator, functioning as a mediator of activity for a number of hormones, including epinephrine, glucagon, and ACTH. Adenosine Cyclic 3',5'-Monophosphate,Adenosine Cyclic 3,5 Monophosphate,Adenosine Cyclic Monophosphate,Adenosine Cyclic-3',5'-Monophosphate,Cyclic AMP, (R)-Isomer,Cyclic AMP, Disodium Salt,Cyclic AMP, Monoammonium Salt,Cyclic AMP, Monopotassium Salt,Cyclic AMP, Monosodium Salt,Cyclic AMP, Sodium Salt,3',5'-Monophosphate, Adenosine Cyclic,AMP, Cyclic,Adenosine Cyclic 3',5' Monophosphate,Cyclic 3',5'-Monophosphate, Adenosine,Cyclic Monophosphate, Adenosine,Cyclic-3',5'-Monophosphate, Adenosine,Monophosphate, Adenosine Cyclic
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D014638 Vanadates Oxyvanadium ions in various states of oxidation. They act primarily as ion transport inhibitors due to their inhibition of Na(+)-, K(+)-, and Ca(+)-ATPase transport systems. They also have insulin-like action, positive inotropic action on cardiac ventricular muscle, and other metabolic effects. Decavanadate,Metavanadate,Orthovanadate,Oxyvanadium,Vanadyl,Monovanadate,Sodium Vanadate,Vanadate,Vanadate, Sodium

Related Publications

C Villar-Palasi, and J J Guinovart, and A M Gómez-Foix, and J E Rodriguez-Gil, and F Bosch
Vanadate inhibits protein degradation in isolated rat hepatocytes.
August 1981, The Journal of biological chemistry,
C Villar-Palasi, and J J Guinovart, and A M Gómez-Foix, and J E Rodriguez-Gil, and F Bosch
Vanadate counteracts glucagon effects in isolated rat hepatocytes.
January 1989, Life sciences,
C Villar-Palasi, and J J Guinovart, and A M Gómez-Foix, and J E Rodriguez-Gil, and F Bosch
Effects of vanadate on receptor-mediated endocytosis of asialoglycoproteins in isolated rat hepatocytes.
December 1989, Biochemical Society transactions,
C Villar-Palasi, and J J Guinovart, and A M Gómez-Foix, and J E Rodriguez-Gil, and F Bosch
Insulin-mimetic effects of vanadate in primary cultures of rat hepatocytes.
September 1988, Diabetes,
C Villar-Palasi, and J J Guinovart, and A M Gómez-Foix, and J E Rodriguez-Gil, and F Bosch
Effects of hemin on rat liver cyclic AMP-dependent protein kinases in cell extracts and intact hepatocytes.
December 1985, Biochimica et biophysica acta,
C Villar-Palasi, and J J Guinovart, and A M Gómez-Foix, and J E Rodriguez-Gil, and F Bosch
Effects of vanadate on the cyclic AMP-protein kinase system in rat liver.
September 1980, Biochemical and biophysical research communications,
C Villar-Palasi, and J J Guinovart, and A M Gómez-Foix, and J E Rodriguez-Gil, and F Bosch
Effects of vanadate on heart protein kinase.
December 1982, Biochemical medicine,
C Villar-Palasi, and J J Guinovart, and A M Gómez-Foix, and J E Rodriguez-Gil, and F Bosch
Effects of vanadate on 6-phosphofructo 2-kinase activity and fructose 2,6-bisphosphate levels in cultured rat hepatocytes.
October 1990, Cell biochemistry and function,
C Villar-Palasi, and J J Guinovart, and A M Gómez-Foix, and J E Rodriguez-Gil, and F Bosch
Hypoxia-reoxygenation differentially stimulates stress-activated protein kinases in primary-cultured rat hepatocytes.
January 2000, Transplant international : official journal of the European Society for Organ Transplantation,
C Villar-Palasi, and J J Guinovart, and A M Gómez-Foix, and J E Rodriguez-Gil, and F Bosch
Protein kinases and phosphatases modulate c-fos expression in rat hepatocytes. Effects of angiotensin II and phorbol myristate acetate.
January 1995, Life sciences,
Copied contents to your clipboard!