Novel essential gene Involved in 16S rRNA processing in Escherichia coli. 2015

Tatsuaki Kurata, and Shinobu Nakanishi, and Masayuki Hashimoto, and Masato Taoka, and Yukiko Yamazaki, and Toshiaki Isobe, and Jun-Ichi Kato
Department of Biological Sciences, Graduate Schools of Science and Engineering, Tokyo Metropolitan University, Tokyo 192-0397, Japan.

Biogenesis of ribosomes is a complex process mediated by many factors. While its transcription proceeds, ribosomal RNA (rRNA) folds itself into a characteristic three-dimensional structure through interaction with ribosomal proteins, during which its ends are processed. Here, we show that the essential protein YqgF, a RuvC family protein with an RNase-H-like motif, is involved in the processing of pre-16S rRNA during ribosome maturation. Indeed, pre-16S rRNA accumulated in cells of a temperature-sensitive yqgF mutant (yqgF(ts)) cultured at a non-permissive temperature. In addition, purified YqgF was shown to process the 5' end of pre-16S rRNA within 70S ribosomes in vitro. Mass spectrometry analysis of the total proteins in the yqgF(ts) mutant cells showed that the expression of genes containing multiple Shine-Dalgarno-like sequences was observed to be lower than in wild type. These results are interpreted to indicate that YqgF is involved in a novel enzymic activity necessary for the processing of pre-16S rRNA, thereby affecting elongation of translation.

UI MeSH Term Description Entries
D009154 Mutation Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations. Mutations
D011499 Protein Processing, Post-Translational Any of various enzymatically catalyzed post-translational modifications of PEPTIDES or PROTEINS in the cell of origin. These modifications include carboxylation; HYDROXYLATION; ACETYLATION; PHOSPHORYLATION; METHYLATION; GLYCOSYLATION; ubiquitination; oxidation; proteolysis; and crosslinking and result in changes in molecular weight and electrophoretic motility. Amino Acid Modification, Post-Translational,Post-Translational Modification,Post-Translational Protein Modification,Posttranslational Modification,Protein Modification, Post-Translational,Amino Acid Modification, Posttranslational,Post-Translational Amino Acid Modification,Post-Translational Modifications,Post-Translational Protein Processing,Posttranslational Amino Acid Modification,Posttranslational Modifications,Posttranslational Protein Processing,Protein Processing, Post Translational,Protein Processing, Posttranslational,Amino Acid Modification, Post Translational,Modification, Post-Translational,Modification, Post-Translational Protein,Modification, Posttranslational,Modifications, Post-Translational,Modifications, Post-Translational Protein,Modifications, Posttranslational,Post Translational Amino Acid Modification,Post Translational Modification,Post Translational Modifications,Post Translational Protein Modification,Post Translational Protein Processing,Post-Translational Protein Modifications,Processing, Post-Translational Protein,Processing, Posttranslational Protein,Protein Modification, Post Translational,Protein Modifications, Post-Translational
D004706 Endodeoxyribonucleases A group of enzymes catalyzing the endonucleolytic cleavage of DNA. They include members of EC 3.1.21.-, EC 3.1.22.-, EC 3.1.23.- (DNA RESTRICTION ENZYMES), EC 3.1.24.- (DNA RESTRICTION ENZYMES), and EC 3.1.25.-.
D004926 Escherichia coli A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc. Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D012269 Ribosomal Proteins Proteins found in ribosomes. They are believed to have a catalytic function in reconstituting biologically active ribosomal subunits. Proteins, Ribosomal,Ribosomal Protein,Protein, Ribosomal
D012270 Ribosomes Multicomponent ribonucleoprotein structures found in the CYTOPLASM of all cells, and in MITOCHONDRIA, and PLASTIDS. They function in PROTEIN BIOSYNTHESIS via GENETIC TRANSLATION. Ribosome
D012323 RNA Processing, Post-Transcriptional Post-transcriptional biological modification of messenger, transfer, or ribosomal RNAs or their precursors. It includes cleavage, methylation, thiolation, isopentenylation, pseudouridine formation, conformational changes, and association with ribosomal protein. Post-Transcriptional RNA Modification,RNA Processing,Post-Transcriptional RNA Processing,Posttranscriptional RNA Processing,RNA Processing, Post Transcriptional,RNA Processing, Posttranscriptional,Modification, Post-Transcriptional RNA,Modifications, Post-Transcriptional RNA,Post Transcriptional RNA Modification,Post Transcriptional RNA Processing,Post-Transcriptional RNA Modifications,Processing, Posttranscriptional RNA,Processing, RNA,RNA Modification, Post-Transcriptional,RNA Modifications, Post-Transcriptional
D012329 RNA, Bacterial Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis. Bacterial RNA
D012336 RNA, Ribosomal, 16S Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis. 16S Ribosomal RNA,16S rRNA,RNA, 16S Ribosomal,Ribosomal RNA, 16S,rRNA, 16S
D020043 Genes, Essential Those genes found in an organism which are necessary for its viability and normal function. Genes, House-keeping,Genes, Housekeeping,Essential Gene,Essential Genes,Gene, Essential,Gene, House-keeping,Gene, Housekeeping,Genes, House keeping,House-keeping Gene,House-keeping Genes,Housekeeping Gene,Housekeeping Genes

Related Publications

Tatsuaki Kurata, and Shinobu Nakanishi, and Masayuki Hashimoto, and Masato Taoka, and Yukiko Yamazaki, and Toshiaki Isobe, and Jun-Ichi Kato
February 1989, Nucleic acids research,
Tatsuaki Kurata, and Shinobu Nakanishi, and Masayuki Hashimoto, and Masato Taoka, and Yukiko Yamazaki, and Toshiaki Isobe, and Jun-Ichi Kato
January 1998, Journal of bacteriology,
Tatsuaki Kurata, and Shinobu Nakanishi, and Masayuki Hashimoto, and Masato Taoka, and Yukiko Yamazaki, and Toshiaki Isobe, and Jun-Ichi Kato
June 1999, Biochemical and biophysical research communications,
Tatsuaki Kurata, and Shinobu Nakanishi, and Masayuki Hashimoto, and Masato Taoka, and Yukiko Yamazaki, and Toshiaki Isobe, and Jun-Ichi Kato
December 1987, Journal of bacteriology,
Tatsuaki Kurata, and Shinobu Nakanishi, and Masayuki Hashimoto, and Masato Taoka, and Yukiko Yamazaki, and Toshiaki Isobe, and Jun-Ichi Kato
February 1999, Environmental microbiology,
Tatsuaki Kurata, and Shinobu Nakanishi, and Masayuki Hashimoto, and Masato Taoka, and Yukiko Yamazaki, and Toshiaki Isobe, and Jun-Ichi Kato
August 2009, Microbiology (Reading, England),
Tatsuaki Kurata, and Shinobu Nakanishi, and Masayuki Hashimoto, and Masato Taoka, and Yukiko Yamazaki, and Toshiaki Isobe, and Jun-Ichi Kato
November 2013, Journal of basic microbiology,
Tatsuaki Kurata, and Shinobu Nakanishi, and Masayuki Hashimoto, and Masato Taoka, and Yukiko Yamazaki, and Toshiaki Isobe, and Jun-Ichi Kato
November 2016, mBio,
Tatsuaki Kurata, and Shinobu Nakanishi, and Masayuki Hashimoto, and Masato Taoka, and Yukiko Yamazaki, and Toshiaki Isobe, and Jun-Ichi Kato
December 2007, Journal of bacteriology,
Tatsuaki Kurata, and Shinobu Nakanishi, and Masayuki Hashimoto, and Masato Taoka, and Yukiko Yamazaki, and Toshiaki Isobe, and Jun-Ichi Kato
July 1997, Journal of bacteriology,
Copied contents to your clipboard!