Nucleoprotein-based indirect enzyme-linked immunosorbent assay (indirect ELISA) for detecting antibodies specific to Ebola virus and Marbug virus. 2014

Yi Huang, and Youjie Zhu, and Mengshi Yang, and Zhenqing Zhang, and Donglin Song, and Zhiming Yuan
Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China.

Full-length nucleoproteins from Ebola and Marburg viruses were expressed as His-tagged recombinant proteins in Escherichia coli and nucleoprotein-based enzyme-linked immunosorbent assays (ELISAs) were established for the detection of antibodies specific to Ebola and Marburg viruses. The ELISAs were evaluated by testing antisera collected from rabbit immunized with Ebola and Marburg virus nucleoproteins. Although little cross-reactivity of antibodies was observed in anti-Ebola virus nucleoprotein rabbit antisera, the highest reactions to immunoglobulin G (IgG) were uniformly detected against the nucleoprotein antigens of homologous viruses. We further evaluated the ELISA's ability to detect antibodies to Ebola and Marburg viruses using human sera samples collected from individuals passing through the Guangdong port of entry. With a threshold set at the mean plus three standard deviations of average optical densities of sera tested, the ELISA systems using these two recombinant nucleoproteins have good sensitivity and specificity. These results demonstrate the usefulness of ELISA for diagnostics as well as ecological and serosurvey studies of Ebola and Marburg virus infection.

UI MeSH Term Description Entries
D008379 Marburg Virus Disease An RNA virus infection of rhesus, vervet, and squirrel monkeys transmissible to man. Marburg Infection,Marburg Virus Infection,Marburg Disease,Marburg Hemorrhagic Fever,Disease, Marburg,Fever, Marburg Hemorrhagic,Hemorrhagic Fever, Marburg,Infection, Marburg,Infection, Marburg Virus,Marburg Infections,Marburg Virus Infections,Virus Infection, Marburg
D009698 Nucleoproteins Proteins conjugated with nucleic acids. Nucleoprotein
D011817 Rabbits A burrowing plant-eating mammal with hind limbs that are longer than its fore limbs. It belongs to the family Leporidae of the order Lagomorpha, and in contrast to hares, possesses 22 instead of 24 pairs of chromosomes. Belgian Hare,New Zealand Rabbit,New Zealand Rabbits,New Zealand White Rabbit,Rabbit,Rabbit, Domestic,Chinchilla Rabbits,NZW Rabbits,New Zealand White Rabbits,Oryctolagus cuniculus,Chinchilla Rabbit,Domestic Rabbit,Domestic Rabbits,Hare, Belgian,NZW Rabbit,Rabbit, Chinchilla,Rabbit, NZW,Rabbit, New Zealand,Rabbits, Chinchilla,Rabbits, Domestic,Rabbits, NZW,Rabbits, New Zealand,Zealand Rabbit, New,Zealand Rabbits, New,cuniculus, Oryctolagus
D004797 Enzyme-Linked Immunosorbent Assay An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed. ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000914 Antibodies, Viral Immunoglobulins produced in response to VIRAL ANTIGENS. Viral Antibodies
D019142 Hemorrhagic Fever, Ebola A highly fatal, acute hemorrhagic fever caused by EBOLAVIRUS. Ebola Hemorrhagic Fever,Ebola Virus Disease,Ebolavirus Infection,Ebola Infection,Ebola Virus Infection,Ebolavirus Infections,Infection, Ebola,Infection, Ebola Virus,Infection, Ebolavirus,Infections, Ebolavirus,Virus Infection, Ebola
D029024 Marburgvirus A genus in the family FILOVIRIDAE consisting of one species (Lake Victoria marburgvirus) with several strains. The genus shows no antigenic cross-reactivity with EBOLAVIRUS. Lake Victoria marburgvirus,Ravn Virus,Frankfurt-Marburg Syndrome Virus,Marburg Virus,Marburg-like Viruses,Frankfurt Marburg Syndrome Virus,Marburg Viruses,Marburg like Viruses,Marburg-like Virus,Marburgviruses,Ravn Viruses,Virus, Marburg,Virus, Ravn
D029043 Ebolavirus A genus in the family FILOVIRIDAE consisting of several distinct species of Ebolavirus, each containing separate strains. These viruses cause outbreaks of a contagious, hemorrhagic disease (HEMORRHAGIC FEVER, EBOLA) in humans, usually with high mortality. Cote d'Ivoire ebola virus,Ebola Virus, Cote d'Ivoire,Ebola Virus, Reston,Ebola Virus, Sudan,Ebola Virus, Zaire,Ebola Virus,Ebola-like Viruses,Reston Ebola Virus,Sudan Ebola Virus,Zaire Ebola Virus,Ebola Viruses,Ebola like Viruses,Ebola-like Virus,Ebolaviruses,Virus, Ebola,Viruses, Ebola

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