Up-regulation of NF45 correlates with Schwann cell proliferation after sciatic nerve crush. 2015

Youhua Wang, and Shiran Zhou, and Hua Xu, and Shixian Yan, and Dawei Xu, and Yi Zhang
Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, People's Republic of China.

Nuclear factor (NF)45 (also known as interleukin enhancer-binding factor (ILF)2), is a transcription factor that interacts with NF90 to regulate gene expression. It has long been implicated in the regulation of cell proliferation. However, the role of NF45 in the process of peripheral nervous system regeneration after injury remains poorly understood. Herein, we investigated the spatiotemporal expression of NF45 in a rat sciatic nerve crush model. We detected the up-regulated expression of NF45 in Schwann cell after sciatic nerve crush. What's more, the expression of the cell proliferation marker proliferating cell nuclear antigen (PCNA) exhibited a similar tendency with that of NF45. In cell cultures, we observed increased expression of NF45 during the process of TNF-α-induced Schwann cell proliferation, whereas the protein level of p21 was down-regulated. Interference of NF45 led to enhanced expression of p21 and also impaired proliferation of Schwan cells. Taken together, our data implicated that NF45 was up-regulated in the sciatic nerve after crush, which was associated with proliferation of Schwann cell.

UI MeSH Term Description Entries
D008297 Male Males
D009409 Nerve Crush Treatment of muscles and nerves under pressure as a result of crush injuries. Crush, Nerve
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012583 Schwann Cells Neuroglial cells of the peripheral nervous system which form the insulating myelin sheaths of peripheral axons. Schwann Cell,Cell, Schwann,Cells, Schwann
D012584 Sciatic Nerve A nerve which originates in the lumbar and sacral spinal cord (L4 to S3) and supplies motor and sensory innervation to the lower extremity. The sciatic nerve, which is the main continuation of the sacral plexus, is the largest nerve in the body. It has two major branches, the TIBIAL NERVE and the PERONEAL NERVE. Nerve, Sciatic,Nerves, Sciatic,Sciatic Nerves
D015854 Up-Regulation A positive regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins. Receptor Up-Regulation,Upregulation,Up-Regulation (Physiology),Up Regulation
D016283 Proto-Oncogene Proteins p21(ras) Cellular proteins encoded by the H-ras, K-ras and N-ras genes. The proteins have GTPase activity and are involved in signal transduction as monomeric GTP-binding proteins. Elevated levels of p21 c-ras have been associated with neoplasia. This enzyme was formerly listed as EC 3.6.1.47. Proto-Oncogene Proteins c-ras,c-Ha-ras p21,c-Ki-ras p21,p21(N-ras),p21(c-Ha-ras),p21(c-Ki-ras),p21(c-ras),p21(ras),ras Proto-Oncogene Protein p21,Proto-Oncogene Protein p21(c-Ha-ras),Proto-Oncogene Protein p21(c-Ki-ras),Proto-Oncogene Protein p21(c-N-ras),Proto-Oncogene Protein p21(ras),Proto-Oncogene Protein ras,c-ras Proteins,p21 c-H-ras,p21 c-Ha-ras,p21 c-K-ras,p21 c-Ki-ras,p21 c-ras,ras Proto-Oncogene Product p21,Proteins c-ras, Proto-Oncogene,Proto Oncogene Protein ras,Proto Oncogene Proteins c ras,c Ha ras p21,c Ki ras p21,c ras Proteins,c-H-ras, p21,c-Ha-ras, p21,c-K-ras, p21,c-Ki-ras, p21,c-ras, Proto-Oncogene Proteins,c-ras, p21,p21 c H ras,p21 c Ha ras,p21 c K ras,p21 c Ki ras,p21 c ras,p21, c-Ha-ras,p21, c-Ki-ras,ras Proto Oncogene Product p21,ras Proto Oncogene Protein p21,ras, Proto-Oncogene Protein
D017207 Rats, Sprague-Dawley A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company. Holtzman Rat,Rats, Holtzman,Sprague-Dawley Rat,Rats, Sprague Dawley,Holtzman Rats,Rat, Holtzman,Rat, Sprague-Dawley,Sprague Dawley Rat,Sprague Dawley Rats,Sprague-Dawley Rats
D049109 Cell Proliferation All of the processes involved in increasing CELL NUMBER including CELL DIVISION. Cell Growth in Number,Cellular Proliferation,Cell Multiplication,Cell Number Growth,Growth, Cell Number,Multiplication, Cell,Number Growth, Cell,Proliferation, Cell,Proliferation, Cellular

Related Publications

Youhua Wang, and Shiran Zhou, and Hua Xu, and Shixian Yan, and Dawei Xu, and Yi Zhang
November 2014, Neurochemical research,
Youhua Wang, and Shiran Zhou, and Hua Xu, and Shixian Yan, and Dawei Xu, and Yi Zhang
June 2018, Neurochemical research,
Youhua Wang, and Shiran Zhou, and Hua Xu, and Shixian Yan, and Dawei Xu, and Yi Zhang
October 2016, Journal of molecular neuroscience : MN,
Youhua Wang, and Shiran Zhou, and Hua Xu, and Shixian Yan, and Dawei Xu, and Yi Zhang
June 2015, Purinergic signalling,
Youhua Wang, and Shiran Zhou, and Hua Xu, and Shixian Yan, and Dawei Xu, and Yi Zhang
September 2016, Biochemical and biophysical research communications,
Youhua Wang, and Shiran Zhou, and Hua Xu, and Shixian Yan, and Dawei Xu, and Yi Zhang
December 2018, Neurochemical research,
Youhua Wang, and Shiran Zhou, and Hua Xu, and Shixian Yan, and Dawei Xu, and Yi Zhang
October 2014, Cellular and molecular neurobiology,
Youhua Wang, and Shiran Zhou, and Hua Xu, and Shixian Yan, and Dawei Xu, and Yi Zhang
May 2016, Biochemical and biophysical research communications,
Youhua Wang, and Shiran Zhou, and Hua Xu, and Shixian Yan, and Dawei Xu, and Yi Zhang
August 2013, Journal of molecular histology,
Youhua Wang, and Shiran Zhou, and Hua Xu, and Shixian Yan, and Dawei Xu, and Yi Zhang
December 2014, Journal of molecular neuroscience : MN,
Copied contents to your clipboard!