Biomaterial Database

cDNA sequence of the long terminal repeat of a PrB strain of the Rous sarcoma virus. 1989

T J Chao

Department of Pathology, National Defence Medical Center, Tri-Service General Hospital.

The cDNA library of the polyA+ RNA from Prague B strain of RSV infected cells was constructed by the use of oligo(dT) primer and the reverse transcription method. The clones containing the LTR sequences were studied. The DNA sequences of several clones were studied and compared with the cDNA sequence of the long terminal repeat (LTR) region of a PrB strain of Rous sarcoma virus (RSV) which had previously been determined. By the analyses of several different cDNA clones, mutations were detected in the R region as well as in the U3 region of the LTR in two different cDNA clones. By comparing the DNA sequences of these cDNA clones with the original viral LTR, the error-prone hypothesis of the RSV reverse transcriptase was confirmed.

UI	MeSH Term	Description	Entries
D008969	Molecular Sequence Data	Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.	Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D012091	Repetitive Sequences, Nucleic Acid	Sequences of DNA or RNA that occur in multiple copies. There are several types: INTERSPERSED REPETITIVE SEQUENCES are copies of transposable elements (DNA TRANSPOSABLE ELEMENTS or RETROELEMENTS) dispersed throughout the genome. TERMINAL REPEAT SEQUENCES flank both ends of another sequence, for example, the long terminal repeats (LTRs) on RETROVIRUSES. Variations may be direct repeats, those occurring in the same direction, or inverted repeats, those opposite to each other in direction. TANDEM REPEAT SEQUENCES are copies which lie adjacent to each other, direct or inverted (INVERTED REPEAT SEQUENCES).	DNA Repetitious Region,Direct Repeat,Genes, Selfish,Nucleic Acid Repetitive Sequences,Repetitive Region,Selfish DNA,Selfish Genes,DNA, Selfish,Repetitious Region, DNA,Repetitive Sequence,DNA Repetitious Regions,DNAs, Selfish,Direct Repeats,Gene, Selfish,Repeat, Direct,Repeats, Direct,Repetitious Regions, DNA,Repetitive Regions,Repetitive Sequences,Selfish DNAs,Selfish Gene
D004247	DNA	A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).	DNA, Double-Stranded,Deoxyribonucleic Acid,ds-DNA,DNA, Double Stranded,Double-Stranded DNA,ds DNA
D001358	Avian Sarcoma Viruses	Group of alpharetroviruses (ALPHARETROVIRUS) producing sarcomata and other tumors in chickens and other fowl and also in pigeons, ducks, and RATS.	Avian Sarcoma Virus B77,Chicken Sarcoma Virus B77,Chicken Tumor 1 Virus,Fujinami sarcoma virus,Sarcoma Viruses, Avian,Avian Sarcoma Virus,Fujinami sarcoma viruses,Sarcoma Virus, Avian,Virus, Avian Sarcoma,Viruses, Avian Sarcoma,sarcoma virus, Fujinami,virus, Fujinami sarcoma,viruses, Fujinami sarcoma
D001483	Base Sequence	The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.	DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA