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To assess the contribution of neutrophil elastase to neutrophil-mediated acute edematous lung injury, isolated rat lungs were perfused with human neutrophil cytoplasts that produce O2 metabolites normally but contain little of the neutrophil elastase usually found in neutrophils. We found that addition of neutrophil cytoplasts and phorbol myristate acetate (PMA) caused less edematous injury in isolated lungs than addition of neutrophils and PMA. However, addition of neutrophil elastase along with neutrophil cytoplasts and PMA increased the amounts of lung injury found in lungs perfused with neutrophil cytoplasts and PMA to levels that equaled the amount of lung injury found after addition of normal neutrophils and PMA. By comparison, addition of purified neutrophil elastase, PMA alone, or human neutrophils alone did not cause lung injury. The results indicate that neutrophil elastase is a necessary component for the maximal development of acute edematous injury in isolated lungs perfused with neutrophils and PMA.
R J McDonald, and
L V Bruckner, and
J E Repine
July 1990,
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R J McDonald, and
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J E Repine
December 1986,
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R J McDonald, and
L V Bruckner, and
J E Repine
April 1994,
The Journal of laboratory and clinical medicine,
R J McDonald, and
L V Bruckner, and
J E Repine
November 1988,
Journal of applied physiology (Bethesda, Md. : 1985),
R J McDonald, and
L V Bruckner, and
J E Repine
May 1986,
Laboratory investigation; a journal of technical methods and pathology,
R J McDonald, and
L V Bruckner, and
J E Repine
February 1999,
The American journal of physiology,
R J McDonald, and
L V Bruckner, and
J E Repine
October 1991,
The American journal of physiology,
R J McDonald, and
L V Bruckner, and
J E Repine
April 1982,
The American review of respiratory disease,
R J McDonald, and
L V Bruckner, and
J E Repine
February 2012,
Critical care medicine,
R J McDonald, and
L V Bruckner, and
J E Repine
January 1998,
American journal of respiratory and critical care medicine,
