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DNA fingerprinting with the oligonucleotide probe (CAC)5/(GTG)5: somatic stability and germline mutations. 1989
P Nürnberg, and
L Roewer, and
H Neitzel, and
K Sperling, and
A Pöpperl, and
J Hundrieser, and
H Pöche, and
C Epplen, and
H Zischler, and
J T Epplen
Institut für Medizinische Genetik des Bereichs Medizin der Charité, Humboldt-Universität, Berlin, German Democratic Republic.
DNA fingerprints were generated from various human somatic tissues and from peripheral blood of 179 children and their 80 parents using (CAC)5/(GTG)5 oligonucleotide probes. Whereas somatic stability of the fingerprint patterns was demonstrated, the average rate for germline mutations was estimated to be approximately 0.001 per DNA locus and gamete, with the three different restriction enzymes used. Seven out of eight mutations observed appeared to be of paternal origin.
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