Biomaterial Database

Two monoclonal antibodies identify thymic-repopulating cells in mouse bone marrow. 1989

G J Spangrude, and J Klein, and S Heimfeld, and Y Aihara, and I L Weissman

Department of Pathology, Stanford University School of Medicine, CA 94305.

The progenitor cells in the bone marrow that home to and repopulate the thymus have been incompletely characterized. In particular, it is not clear whether thymocytes differentiate directly from pluripotent hemopoietic stem cells that seed to the thymus, or whether T lymphoid-committed stem cells (prothymocytes) arise in the bone marrow before the thymic migration. In order to resolve this question, we have used mAb specific for cell-surface Ag to identify the bone marrow cells which can seed to and repopulate the thymus of irradiated mice. We report here that the majority of thymic-repopulating cells in mouse bone marrow express low levels of the Thy-1 Ag (Thy-1lo) plus high levels of a newly described Ag termed stem cell Ag (Sca-1). Two distinct populations of thymic-repopulating Thy-1loSca-1+ cells in mouse bone marrow can be discriminated based on expression of any of a number of hemolymphoid lineage-specific (Lin) markers. Thus, Thy-1loLin-Sca-1+ and Thy-1loLin+Sca-1+ fractions of bone marrow contain thymic-repopulating cells. A second Ag, stem cell Ag-2 (Sca-2), is expressed by Thy-1loLin+Sca-1+ cells but not by Thy-1loLin-Sca-1+ cells. The Thy-1loLin-Sca-1+ fraction expresses intermediate levels of the phagocyte glycoprotein-1 Ag, and comprises 30% of the Thy-1loLin- bone marrow cells, which have previously been shown to be highly enriched in pluripotent hemopoietic stem cells. By facilitating the isolation of highly purified subpopulations of bone marrow cells that can repopulate the thymus, Sca-1 and Sca-2 should provide an experimental tool for describing the developmental potential of such bone marrow subsets.

UI	MeSH Term	Description	Entries
D008810	Mice, Inbred C57BL	One of the first INBRED MOUSE STRAINS to be sequenced. This strain is commonly used as genetic background for transgenic mouse models. Refractory to many tumors, this strain is also preferred model for studying role of genetic variations in development of diseases.	Mice, C57BL,Mouse, C57BL,Mouse, Inbred C57BL,C57BL Mice,C57BL Mice, Inbred,C57BL Mouse,C57BL Mouse, Inbred,Inbred C57BL Mice,Inbred C57BL Mouse
D010641	Phenotype	The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.	Phenotypes
D012038	Regeneration	The physiological renewal, repair, or replacement of tissue.	Endogenous Regeneration,Regeneration, Endogenous,Regenerations
D001853	Bone Marrow	The soft tissue filling the cavities of bones. Bone marrow exists in two types, yellow and red. Yellow marrow is found in the large cavities of large bones and consists mostly of fat cells and a few primitive blood cells. Red marrow is a hematopoietic tissue and is the site of production of erythrocytes and granular leukocytes. Bone marrow is made up of a framework of connective tissue containing branching fibers with the frame being filled with marrow cells.	Marrow,Red Marrow,Yellow Marrow,Marrow, Bone,Marrow, Red,Marrow, Yellow
D002469	Cell Separation	Techniques for separating distinct populations of cells.	Cell Isolation,Cell Segregation,Isolation, Cell,Cell Isolations,Cell Segregations,Cell Separations,Isolations, Cell,Segregation, Cell,Segregations, Cell,Separation, Cell,Separations, Cell
D006412	Hematopoietic Stem Cells	Progenitor cells from which all blood cells derived. They are found primarily in the bone marrow and also in small numbers in the peripheral blood.	Colony-Forming Units, Hematopoietic,Progenitor Cells, Hematopoietic,Stem Cells, Hematopoietic,Hematopoietic Progenitor Cells,Cell, Hematopoietic Progenitor,Cell, Hematopoietic Stem,Cells, Hematopoietic Progenitor,Cells, Hematopoietic Stem,Colony Forming Units, Hematopoietic,Colony-Forming Unit, Hematopoietic,Hematopoietic Colony-Forming Unit,Hematopoietic Colony-Forming Units,Hematopoietic Progenitor Cell,Hematopoietic Stem Cell,Progenitor Cell, Hematopoietic,Stem Cell, Hematopoietic,Unit, Hematopoietic Colony-Forming,Units, Hematopoietic Colony-Forming
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D000911	Antibodies, Monoclonal	Antibodies produced by a single clone of cells.	Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal
D000937	Antigen-Antibody Reactions	The processes triggered by interactions of ANTIBODIES with their ANTIGENS.	Antigen Antibody Reactions,Antigen-Antibody Reaction,Reaction, Antigen-Antibody,Reactions, Antigen-Antibody
D000954	Antigens, Surface	Antigens on surfaces of cells, including infectious or foreign cells or viruses. They are usually protein-containing groups on cell membranes or walls and may be isolated.	Cell Surface Antigens,Surface Antigens,Surface Markers, Immunological,Cell Surface Antigen,Immunologic Surface Markers,Markers, Immunological Surface,Surface Antigen,Surface Markers, Immunologic,Antigen, Cell Surface,Antigen, Surface,Antigens, Cell Surface,Immunological Surface Markers,Markers, Immunologic Surface,Surface Antigen, Cell,Surface Antigens, Cell