BIOMAT Database Logo BIOMAT Database Logo

Quantitative Screening of Agrochemical Residues in Fruits and Vegetables by Buffered Ethyl Acetate Extraction and LC-MS/MS Analysis. 2015

Manjusha R Jadhav, and Dasharath P Oulkar, and Ahammed Shabeer T P, and Kaushik Banerjee
National Referral Laboratory, ICAR-National Research Centre for Grapes, P.O. Manjri Farm, Pune 412307, India.

A buffered ethyl acetate extraction method is proposed for the simultaneous analysis of 296 agrochemicals in a wide range of fruit and vegetable matrices by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The optimized quantity of acetate buffer (1% acetic acid + 0.5 g of sodium acetate per 10 g of sample) adjusted the pH of each test matrix to 5-6, which in turn significantly improved recoveries of acidic and basic compounds. The role of diethylene glycol (used in the evaporation step) on signal suppression of certain compounds was evaluated, and its quantity was optimized to minimize such an effect. The method was validated in grape, mango, drumstick, bitter gourd, capsicum, curry leaf, and okra as per the DG-SANCO/12571/2013 guidelines. Recoveries in the fortification range of 1-40 μg/kg were within 70-120% with associated relative standard deviations below 20% for most of the compounds. The method has potential for regulatory and commercial applications with a generic approach.

UI MeSH Term Description Entries
D002851 Chromatography, High Pressure Liquid Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D005506 Food Contamination The presence in food of harmful, unpalatable, or otherwise objectionable foreign substances, e.g. chemicals, microorganisms or diluents, before, during, or after processing or storage. Food Adulteration,Adulteration, Food,Adulterations, Food,Contamination, Food,Contaminations, Food,Food Adulterations,Food Contaminations
D005591 Chemical Fractionation Separation of a mixture in successive stages, each stage removing from the mixture some proportion of one of the substances, for example by differential solubility in water-solvent mixtures. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Fractionation, Chemical,Chemical Fractionations,Fractionations, Chemical
D005638 Fruit The fleshy or dry ripened ovary of a plant, enclosing the seed or seeds. Berries,Legume Pod,Plant Aril,Plant Capsule,Aril, Plant,Arils, Plant,Berry,Capsule, Plant,Capsules, Plant,Fruits,Legume Pods,Plant Arils,Plant Capsules,Pod, Legume,Pods, Legume
D014675 Vegetables A food group comprised of EDIBLE PLANTS or their parts. Vegetable
D016573 Agrochemicals Chemicals used in agriculture. These include pesticides, fumigants, fertilizers, plant hormones, steroids, antibiotics, mycotoxins, etc. Agrichemical,Agricultural Chemical,Agricultural Chemicals,Agrochemical,Agrichemicals,Chemicals, Agricultural,Chemical, Agricultural
D053719 Tandem Mass Spectrometry A mass spectrometry technique using two (MS/MS) or more mass analyzers. With two in tandem, the precursor ions are mass-selected by a first mass analyzer, and focused into a collision region where they are then fragmented into product ions which are then characterized by a second mass analyzer. A variety of techniques are used to separate the compounds, ionize them, and introduce them to the first mass analyzer. For example, for in GC-MS/MS, GAS CHROMATOGRAPHY-MASS SPECTROMETRY is involved in separating relatively small compounds by GAS CHROMATOGRAPHY prior to injecting them into an ionization chamber for the mass selection. Mass Spectrometry-Mass Spectrometry,Mass Spectrometry Mass Spectrometry,Mass Spectrometry, Tandem

Related Publications

Manjusha R Jadhav, and Dasharath P Oulkar, and Ahammed Shabeer T P, and Kaushik Banerjee
Targeted screening and safety evaluation of 276 agrochemical residues in raisins using buffered ethyl acetate extraction and liquid chromatography-tandem mass spectrometry analysis.
October 2017, Chemosphere,
Manjusha R Jadhav, and Dasharath P Oulkar, and Ahammed Shabeer T P, and Kaushik Banerjee
LC-MS/MS determination of pesticide residues in fruits and vegetables.
July 2017, Journal of environmental science and health. Part. B, Pesticides, food contaminants, and agricultural wastes,
Manjusha R Jadhav, and Dasharath P Oulkar, and Ahammed Shabeer T P, and Kaushik Banerjee
Surveillance of Chlorantraniliprole Residues in Vegetables and Fruits Using LC-MS/MS.
January 2018, Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan,
Manjusha R Jadhav, and Dasharath P Oulkar, and Ahammed Shabeer T P, and Kaushik Banerjee
Analysis of forchlorfenuron in vegetables by LC/TOF-MS after extraction with the buffered QuEChERS method.
March 2010, Journal of agricultural and food chemistry,
Manjusha R Jadhav, and Dasharath P Oulkar, and Ahammed Shabeer T P, and Kaushik Banerjee
Analysis of methoxyfenozide residues in fruits, vegetables, and mint by liquid chromatography-tandem mass spectrometry (LC-MS/MS).
February 2004, Journal of agricultural and food chemistry,
Manjusha R Jadhav, and Dasharath P Oulkar, and Ahammed Shabeer T P, and Kaushik Banerjee
Determination of imidacloprid and benzimidazole residues in fruits and vegetables by liquid chromatography-mass spectrometry after ethyl acetate multiresidue extraction.
January 2000, Journal of AOAC International,
Manjusha R Jadhav, and Dasharath P Oulkar, and Ahammed Shabeer T P, and Kaushik Banerjee
Recent Modifications and Validation of QuEChERS-dSPE Coupled to LC-MS and GC-MS Instruments for Determination of Pesticide/Agrochemical Residues in Fruits and Vegetables: Review.
August 2018, Journal of chromatographic science,
Manjusha R Jadhav, and Dasharath P Oulkar, and Ahammed Shabeer T P, and Kaushik Banerjee
Method validation and analysis of nine dithiocarbamates in fruits and vegetables by LC-MS/MS.
January 2013, Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment,
Manjusha R Jadhav, and Dasharath P Oulkar, and Ahammed Shabeer T P, and Kaushik Banerjee
LC-MS/MS Analysis of Choline Compounds in Japanese-Cultivated Vegetables and Fruits.
July 2020, Foods (Basel, Switzerland),
Manjusha R Jadhav, and Dasharath P Oulkar, and Ahammed Shabeer T P, and Kaushik Banerjee
Validation of an efficient method for the determination of pesticide residues in fruits and vegetables using ethyl acetate for extraction.
January 2007, Journal of environmental science and health. Part. B, Pesticides, food contaminants, and agricultural wastes,
Copied contents to your clipboard!