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The intracellular routes taken by aminopeptidase N, an apically expressed enzyme in the enterocyte, was followed in small intestinal cultures of pig using either immunoelectron microscopy (immunogold labeling) or continuous labeling with [35S]methionine. Aminopeptidase N was found in the microvillar membrane, the Golgi complex, apical small smooth vesicles, and various acidic lysosomal/endosomal-like organelles. By culturing mucosal explants in the presence of either cycloheximide or (3-(2,4-dinitroanilino)-3-amino-N-methylpropylamine) (DAMP) it was demonstrated that the apical small smooth vesicles are exocytotic and that the low pH in the acid compartments is of no importance for intracellular transport and correct sorting of aminopeptidase N. Furthermore, our results show that the majority of the aminopeptidase N in the lysosomal/endosomal-like compartments is newly synthesized.
G H Hansen, and
E M Danielsen, and
H Sjöström, and
O Norén
January 1992,
Molecular neurobiology,
G H Hansen, and
E M Danielsen, and
H Sjöström, and
O Norén
July 2018,
Cell reports,
G H Hansen, and
E M Danielsen, and
H Sjöström, and
O Norén
January 1985,
Journal of biochemistry,
G H Hansen, and
E M Danielsen, and
H Sjöström, and
O Norén
July 1982,
The Journal of investigative dermatology,
G H Hansen, and
E M Danielsen, and
H Sjöström, and
O Norén
January 1989,
Revisiones sobre biologia celular : RBC,
G H Hansen, and
E M Danielsen, and
H Sjöström, and
O Norén
January 1985,
Progress in clinical and biological research,
G H Hansen, and
E M Danielsen, and
H Sjöström, and
O Norén
October 1984,
Collagen and related research,
G H Hansen, and
E M Danielsen, and
H Sjöström, and
O Norén
October 1994,
Journal of virology,
G H Hansen, and
E M Danielsen, and
H Sjöström, and
O Norén
March 1995,
European journal of cell biology,
G H Hansen, and
E M Danielsen, and
H Sjöström, and
O Norén
May 2023,
Drug delivery and translational research,
