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3H-Prazosin was used to demonstrate alpha 1-adrenoceptors in rat nasal mucosa. Specific binding is saturable and occurs to a homogeneous class of binding sites with high affinity (Kd = 0.07 +/- 0.01 nmol/l and with a receptor density of 0.36 +/- 0.02 pmol/g tissue or 14 +/- 1 fmol/mg protein. Kinetic experiments resulted in a Kd-value of 0.03 nmol/l. The binding is stereoselectively inhibited by epinephrine enantiomers. The antagonist prazosin inhibits 3H-Prazosin binding with high affinity, in contrast to yohimbine, classifying the binding sites as alpha 1-adrenoceptors. Inhibition experiments with WB4101 indicated the presence of alpha 1a- (31 +/- 9%) and alpha 1b-adrenoceptor subtypes in the rat nasal mucosa. The order of potencies of agonists determined in competition experiments was (-)epinephrine greater than (+)epinephrine greater than (-)phenylephrine.
Y J van Megen, and
A B Klaassen, and
J F Rodrigues de Miranda, and
R T Wentges, and
C A van Ginneken
January 2005,
American journal of rhinology,
Y J van Megen, and
A B Klaassen, and
J F Rodrigues de Miranda, and
R T Wentges, and
C A van Ginneken
January 1988,
Archives of oto-rhino-laryngology,
Y J van Megen, and
A B Klaassen, and
J F Rodrigues de Miranda, and
R T Wentges, and
C A van Ginneken
June 1986,
British journal of pharmacology,
Y J van Megen, and
A B Klaassen, and
J F Rodrigues de Miranda, and
R T Wentges, and
C A van Ginneken
August 1991,
The Journal of laryngology and otology,
Y J van Megen, and
A B Klaassen, and
J F Rodrigues de Miranda, and
R T Wentges, and
C A van Ginneken
February 2001,
European journal of pharmacology,
Y J van Megen, and
A B Klaassen, and
J F Rodrigues de Miranda, and
R T Wentges, and
C A van Ginneken
January 1990,
European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery,
Y J van Megen, and
A B Klaassen, and
J F Rodrigues de Miranda, and
R T Wentges, and
C A van Ginneken
January 1985,
Journal of neurochemistry,
Y J van Megen, and
A B Klaassen, and
J F Rodrigues de Miranda, and
R T Wentges, and
C A van Ginneken
July 1995,
European journal of pharmacology,
Y J van Megen, and
A B Klaassen, and
J F Rodrigues de Miranda, and
R T Wentges, and
C A van Ginneken
May 1980,
Brain research,
Y J van Megen, and
A B Klaassen, and
J F Rodrigues de Miranda, and
R T Wentges, and
C A van Ginneken
October 1979,
Life sciences,
