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Xenopus oocytes have been shown to faithfully translate, process, and secrete a number of secretory proteins after the injection of heterologous mRNAs. The oocyte has the capacity to perform a variety of posttranslational protein modifications but has been reported to be incapable of carrying out certain two-step cleavages which proceed via propeptide intermediates. We examined the ability of the oocyte to process preproPTH after the injection of parathyroid mRNA. Microinjected oocytes secreted material which could be detected in a sensitive cytochemical bioassay for PTH. This activity paralleled that of the PTH standard in the assay and was entirely eliminated by a competitive inhibitor of PTH binding, by preincubation with an anti-PTH antiserum, and by coinjecting oocytes with an oligonucleotide mixture complementary to PTH sequences. Immunoprecipitable proPTH and PTH were present in oocyte homogenates, but oocyte-conditioned medium contained only mature PTH(1-84). We conclude that the Xenopus oocyte is capable of accurately processing preproPTH to the mature secretory form of the peptide.
K Ikeda, and
J T Posillico, and
M B Boyle, and
A C Webb, and
E M Brown, and
A E Broadus
January 1985,
CRC critical reviews in biochemistry,
K Ikeda, and
J T Posillico, and
M B Boyle, and
A C Webb, and
E M Brown, and
A E Broadus
July 1981,
Nucleic acids research,
K Ikeda, and
J T Posillico, and
M B Boyle, and
A C Webb, and
E M Brown, and
A E Broadus
May 1978,
La semaine des hopitaux : organe fonde par l'Association d'enseignement medical des hopitaux de Paris,
K Ikeda, and
J T Posillico, and
M B Boyle, and
A C Webb, and
E M Brown, and
A E Broadus
July 2001,
Pharmaceutical research,
K Ikeda, and
J T Posillico, and
M B Boyle, and
A C Webb, and
E M Brown, and
A E Broadus
September 1984,
Clinical endocrinology,
K Ikeda, and
J T Posillico, and
M B Boyle, and
A C Webb, and
E M Brown, and
A E Broadus
January 1984,
Nature,
K Ikeda, and
J T Posillico, and
M B Boyle, and
A C Webb, and
E M Brown, and
A E Broadus
January 1998,
Methods in cell biology,
K Ikeda, and
J T Posillico, and
M B Boyle, and
A C Webb, and
E M Brown, and
A E Broadus
January 1982,
Advances in experimental medicine and biology,
K Ikeda, and
J T Posillico, and
M B Boyle, and
A C Webb, and
E M Brown, and
A E Broadus
February 1983,
Analytical biochemistry,
K Ikeda, and
J T Posillico, and
M B Boyle, and
A C Webb, and
E M Brown, and
A E Broadus
March 1979,
European journal of biochemistry,
