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[Comparison of immunoprotection between vaccination with meq-deleted Marek's disease virus and vaccine strain CVI988/Rispens]. 2014

Luntao Duan, and Shuai Su, and Yixin Wang, and Sifei Li, and Peng Sun, and Wenqing Chen, and Zhizhong Cui

OBJECTIVE To evaluate and compare the immunoprotection between a meq-deleted Marek's disease virus (MDV) and CVI988/Rispens against MDV very virulent strain GX0101. METHODS In total 120 one-day-old SPF chickens were divided into 4 groups (30 each) and kept in 5 isolators with positive pressure-filtered air. At 1 day of age, 2000 PFU of SC9-1 was inoculated subcutaneously into each bird in group 1; 2000 PFU of commercial vaccine CVI988/ Rispens was inoculated subcutaneously into each bird in group 2. No viral challenge was made in group 3 and 4 as controls. Five days later chickens in group 1, 2, 3 were challenged intra-abdominally with 2000 PFU of very virulent MDV strain GX0101. During 90 days after challenge, all dead birds were recorded and checked for necropsy. The tumor-suspected tissues were examined by histopathological biopsy. The antibody titers induced by AIV and NDV vaccination and propagation dynamics of MDV GX0101 were detected. At the same time, parallel tests were performed on Hy-Line Brown chickens containing MDV maternal antibody. RESULTS SC9-1 stain provided 100% protective efficiency against very virulent GX0101 challenge in SPF and Hy-Line Brown chickens. CVI988/Rispens provided 86. 7% protective efficiency against very virulent GX0101 challenge in SPF chickens and 93% in Hy-Line Brown chickens. Challenge with GX0101 caused 53.3% mortality and 16.7% of birds with gross tumors in SPF chickens while there was 36.7% mortality and 16.7% of birds with gross tumors in Hy-Line Brown chickens, and there was no tumor lesion in histopathological biopsy in control group. The results of qPCR demonstrated that the copies of GX0101 viral genomes in SC9-1 vaccinated chickens was lower than CVI988/Rispens vaccinated chickens in lymphocyte and feather follicle DNA. The results of hemagglutination inhibition test demonstrated that antibody titers of AIV and NDV was higher in SC9-1 vaccinated chickens than that in CVI988/Rispens vaccinated chickens. CONCLUSIONS SC9-1 stain's immunoprotection against MDV is more effective than CVI988/Rispens strain's both in SPF chickens and commercial Hy-Line Brown chickens containing maternal antibody.

UI MeSH Term Description Entries
D008380 Marek Disease A transmissible viral disease of birds caused by avian herpesvirus 2 (HERPESVIRUS 2, GALLID) and other MARDIVIRUS. There is lymphoid cell infiltration or lymphomatous tumor formation in the peripheral nerves and gonads, but may also involve visceral organs, skin, muscle, and the eye. Fowl Paralysis,Marek's Disease,Fowl Paralyses,Mareks Disease,Paralyses, Fowl,Paralysis, Fowl
D008381 Herpesvirus 2, Gallid The type species of the genus MARDIVIRUS in the family HERPESVIRIDAE. It is the etiologic agent of MAREK DISEASE, infecting domestic fowl and wild birds. Fowl Paralysis Virus,Marek's Disease Herpesvirus 1,Marek's Disease Virus Serotype 1,Neurolymphomatosis Virus,Gallid Herpesvirus 2,Herpesvirus 2 (gamma), Gallid,Marek Disease Herpesvirus 1,Fowl Paralysis Viruses,Neurolymphomatosis Viruses,Paralysis Virus, Fowl,Paralysis Viruses, Fowl
D009856 Oncogene Proteins, Viral Products of viral oncogenes, most commonly retroviral oncogenes. They usually have transforming and often protein kinase activities. Viral Oncogene Proteins,Viral Transforming Proteins,v-onc Proteins,Transforming Proteins, Viral,v onc Proteins
D011201 Poultry Diseases Diseases of birds which are raised as a source of meat or eggs for human consumption and are usually found in barnyards, hatcheries, etc. The concept is differentiated from BIRD DISEASES which is for diseases of birds not considered poultry and usually found in zoos, parks, and the wild. Disease, Poultry,Diseases, Poultry,Poultry Disease
D002645 Chickens Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA. Gallus gallus,Gallus domesticus,Gallus gallus domesticus,Chicken
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000914 Antibodies, Viral Immunoglobulins produced in response to VIRAL ANTIGENS. Viral Antibodies
D014611 Vaccination Administration of vaccines to stimulate the host's immune response. This includes any preparation intended for active immunological prophylaxis. Immunization, Active,Active Immunization,Active Immunizations,Immunizations, Active,Vaccinations
D014765 Viral Vaccines Suspensions of attenuated or killed viruses administered for the prevention or treatment of infectious viral disease. Viral Vaccine,Vaccine, Viral,Vaccines, Viral
D017353 Gene Deletion A genetic rearrangement through loss of segments of DNA or RNA, bringing sequences which are normally separated into close proximity. This deletion may be detected using cytogenetic techniques and can also be inferred from the phenotype, indicating a deletion at one specific locus. Deletion, Gene,Deletions, Gene,Gene Deletions

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