The identification of Candida albicans strains by restriction fragment length polymorphism analysis of DNA. 1989

R A Smith, and C A Hitchcock, and E G Evans, and C J Lacey, and D J Adams
Department of Microbiology, University of Leeds, U.K.

Digestion of total DNA from Candida albicans isolates with the restriction enzyme HinfI generated simple, readily interpretable band patterns on ethidium bromide-stained gels. The procedure, which was highly reproducible, was used to detect restriction fragment length polymorphisms in 47 isolates of this species.

UI MeSH Term Description Entries
D012150 Polymorphism, Restriction Fragment Length Variation occurring within a species in the presence or length of DNA fragment generated by a specific endonuclease at a specific site in the genome. Such variations are generated by mutations that create or abolish recognition sites for these enzymes or change the length of the fragment. RFLP,Restriction Fragment Length Polymorphism,RFLPs,Restriction Fragment Length Polymorphisms
D002176 Candida albicans A unicellular budding fungus which is the principal pathogenic species causing CANDIDIASIS (moniliasis). Candida albicans var. stellatoidea,Candida stellatoidea,Dematium albicans,Monilia albicans,Myceloblastanon albicans,Mycotorula albicans,Parasaccharomyces albicans,Procandida albicans,Procandida stellatoidea,Saccharomyces albicans,Syringospora albicans
D004271 DNA, Fungal Deoxyribonucleic acid that makes up the genetic material of fungi. Fungal DNA
D004587 Electrophoresis, Agar Gel Electrophoresis in which agar or agarose gel is used as the diffusion medium. Electrophoresis, Agarose Gel,Agar Gel Electrophoresis,Agarose Gel Electrophoresis,Gel Electrophoresis, Agar,Gel Electrophoresis, Agarose
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D015252 Deoxyribonucleases, Type II Site-Specific Enzyme systems containing a single subunit and requiring only magnesium for endonucleolytic activity. The corresponding modification methylases are separate enzymes. The systems recognize specific short DNA sequences and cleave either within, or at a short specific distance from, the recognition sequence to give specific double-stranded fragments with terminal 5'-phosphates. Enzymes from different microorganisms with the same specificity are called isoschizomers. EC 3.1.21.4. DNA Restriction Enzymes, Type II,DNase, Site-Specific, Type II,Restriction Endonucleases, Type II,Type II Restriction Enzymes,DNase, Site Specific, Type II,Deoxyribonucleases, Type II, Site Specific,Deoxyribonucleases, Type II, Site-Specific,Site-Specific DNase, Type II,Type II Site Specific DNase,Type II Site Specific Deoxyribonucleases,Type II Site-Specific DNase,Type II Site-Specific Deoxyribonucleases,Deoxyribonucleases, Type II Site Specific,Site Specific DNase, Type II

Related Publications

R A Smith, and C A Hitchcock, and E G Evans, and C J Lacey, and D J Adams
July 1991, Journal of clinical microbiology,
R A Smith, and C A Hitchcock, and E G Evans, and C J Lacey, and D J Adams
October 1989, Journal of bacteriology,
R A Smith, and C A Hitchcock, and E G Evans, and C J Lacey, and D J Adams
September 1995, Journal of clinical microbiology,
R A Smith, and C A Hitchcock, and E G Evans, and C J Lacey, and D J Adams
May 2001, Current protocols in human genetics,
R A Smith, and C A Hitchcock, and E G Evans, and C J Lacey, and D J Adams
January 1997, The Indian journal of medical research,
R A Smith, and C A Hitchcock, and E G Evans, and C J Lacey, and D J Adams
January 2001, The Journal of eukaryotic microbiology,
R A Smith, and C A Hitchcock, and E G Evans, and C J Lacey, and D J Adams
January 1998, Research in veterinary science,
R A Smith, and C A Hitchcock, and E G Evans, and C J Lacey, and D J Adams
January 1988, Gene analysis techniques,
R A Smith, and C A Hitchcock, and E G Evans, and C J Lacey, and D J Adams
March 1999, Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas,
R A Smith, and C A Hitchcock, and E G Evans, and C J Lacey, and D J Adams
August 1991, FEMS microbiology letters,
Copied contents to your clipboard!