-transparent.png){kind=logo}
A monoclonal antibody against human epidermal growth factor (hEGF) was obtained from a mouse hybridoma cell line. The purified monoclonal antibody from the ascites fluid of a mouse injected with one of the cell lines was specific for hEGF and did not cross-react with mouse EGF (mEGF). Its Kd value for hEGF was 1.4 X 10(-9) M. This monoclonal antibody inhibited the biological activities of hEGF, including its binding to the receptor of BALB/3T3 cells and its stimulation of DNA synthesis in the cells, but did not affect the activities of mEGF. The monoclonal antibody completely inhibited DNA synthesis stimulated by human urine from a patient without a tumor, but only partially inhibited the stimulatory activity in urine from a tumor-bearing patient.
S Ikuta, and
Y Yamada, and
Y Yoshitake, and
K Nishikawa
January 1983,
Hybridoma,
S Ikuta, and
Y Yamada, and
Y Yoshitake, and
K Nishikawa
January 1987,
Methods in enzymology,
S Ikuta, and
Y Yamada, and
Y Yoshitake, and
K Nishikawa
January 1982,
Journal of cellular biochemistry,
S Ikuta, and
Y Yamada, and
Y Yoshitake, and
K Nishikawa
January 1994,
European journal of cancer (Oxford, England : 1990),
S Ikuta, and
Y Yamada, and
Y Yoshitake, and
K Nishikawa
October 2004,
Acta pharmacologica Sinica,
S Ikuta, and
Y Yamada, and
Y Yoshitake, and
K Nishikawa
November 2016,
Biochemical and biophysical research communications,
S Ikuta, and
Y Yamada, and
Y Yoshitake, and
K Nishikawa
January 1987,
Methods in enzymology,
S Ikuta, and
Y Yamada, and
Y Yoshitake, and
K Nishikawa
December 2000,
The New England journal of medicine,
S Ikuta, and
Y Yamada, and
Y Yoshitake, and
K Nishikawa
February 2001,
Cancer biotherapy & radiopharmaceuticals,
S Ikuta, and
Y Yamada, and
Y Yoshitake, and
K Nishikawa
January 1991,
Cancer immunology, immunotherapy : CII,
