[Study of microbial contamination of processed fresh vegetables and lettuce]. 2014

N R efimochkina, and I B Bykova, and S Iu Batishcheva, and L P Minaeva, and Iu M Markova, and Iu V Korotkevich, and G Iu Shilov, and S A Sheveleva

Investigations of microbial contamination and species composition of the Enterobacteriaceae family in fresh vegetables and lettuce has been conducted. The objects of study were new types of fresh ready-to-eat vegetable foods - salads, sliced vegetables and mixtures thereof, sampled at the main stages of production, including washing, antimicrobial treatment with sodium hypochlorite, and packaging in the film under vacuum. Quantitative analysis of Enterobacteriaceae levels in fresh and packaged vegetables and salads showed that their part in the total amount of microbial contaminants is large enough. Average Enterobacteriaceae content ranged from 2,14 to 3,34 lg cfu/g, reaching in some samples values 4,38-4,74 lg, comparable with the levels of total bacteria. Considerable species diversity of microflora contaminating ready-to-eat vegetable products has been found. Bacteria of the genera Enterobactel; Pantoea, Citrobacter, Serratia, Pseudomonas, Kluyvera, Klebsiella, Escherichia, Rahnella, Acinetobacter were found in the salads and sliced vegetables. In the tested samples most frequently detected Enterobacter spp. - 37% of identified strains and Pantoea spp - 25% of strains. The data on the composition and levels of microbial contaminants in vegetable and salad products highlight not only the need to monitor coliform bacteria - traditional indicators of faecal contamination of raw materials, but also the need to introduce criteria for the amount of Enterobacteriaceae.

UI MeSH Term Description Entries
D005506 Food Contamination The presence in food of harmful, unpalatable, or otherwise objectionable foreign substances, e.g. chemicals, microorganisms or diluents, before, during, or after processing or storage. Food Adulteration,Adulteration, Food,Adulterations, Food,Contamination, Food,Contaminations, Food,Food Adulterations,Food Contaminations
D005516 Food Microbiology The presence of bacteria, viruses, and fungi in food and food products. This term is not restricted to pathogenic organisms: the presence of various non-pathogenic bacteria and fungi in cheeses and wines, for example, is included in this concept. Microbiology, Food
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001419 Bacteria One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive. Eubacteria
D018545 Lactuca Any of the various plants of the genus Lactuca, especially L. sativa, cultivated for its edible leaves. (From American Heritage Dictionary, 2d ed) Iceberg Lettuce,Lactuca sativa,Lettuce,Iceberg Lettuces,Lettuce, Iceberg,Lettuces,Lettuces, Iceberg

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