-transparent.png){kind=logo}
A temperature-sensitive mutant, designated tsFT101, was isolated from a mouse mammary carcinoma cell line, FM3A, and given an initial characterization. In this cell line, cytokinesis was blocked at a non-permissive temperature (39 degrees C), but DNA synthesis and nuclear division proceeded normally for at least 24 h at 39 degrees C as detected respectively by autoradiography and cytofluorometric analysis. As a result, multinucleate cells accumulated at 39 degrees C (more than 95% in 36 h). When the culture was returned to a permissive temperature (33 degrees C) after 24 h of arrest at 39 degrees C, cytokinesis was resumed and there was a rapid decrease in the number of multinucleate cells. At 39 degrees C, tsFT101 cells had less F-actin than cells at 33 degrees C, indicative of the existence of an abnormality in actin polymerization in this mutant.
H Yasumitsu, and
F Hanaoka, and
H Yasuda, and
Y Murakami, and
T Enomoto, and
M Yamada
March 1985,
Proceedings of the National Academy of Sciences of the United States of America,
H Yasumitsu, and
F Hanaoka, and
H Yasuda, and
Y Murakami, and
T Enomoto, and
M Yamada
January 1982,
Experimental cell research,
H Yasumitsu, and
F Hanaoka, and
H Yasuda, and
Y Murakami, and
T Enomoto, and
M Yamada
July 1976,
Experimental cell research,
H Yasumitsu, and
F Hanaoka, and
H Yasuda, and
Y Murakami, and
T Enomoto, and
M Yamada
February 1998,
Experimental cell research,
H Yasumitsu, and
F Hanaoka, and
H Yasuda, and
Y Murakami, and
T Enomoto, and
M Yamada
February 1998,
Biochemical and biophysical research communications,
H Yasumitsu, and
F Hanaoka, and
H Yasuda, and
Y Murakami, and
T Enomoto, and
M Yamada
April 1980,
Experimental cell research,
H Yasumitsu, and
F Hanaoka, and
H Yasuda, and
Y Murakami, and
T Enomoto, and
M Yamada
August 1993,
The Journal of biological chemistry,
H Yasumitsu, and
F Hanaoka, and
H Yasuda, and
Y Murakami, and
T Enomoto, and
M Yamada
November 1986,
Somatic cell and molecular genetics,
H Yasumitsu, and
F Hanaoka, and
H Yasuda, and
Y Murakami, and
T Enomoto, and
M Yamada
October 1991,
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme,
H Yasumitsu, and
F Hanaoka, and
H Yasuda, and
Y Murakami, and
T Enomoto, and
M Yamada
January 1990,
The Journal of biological chemistry,
