An Rtf2 Domain-Containing Protein Influences Pre-mRNA Splicing and Is Essential for Embryonic Development in Arabidopsis thaliana. 2015

Taku Sasaki, and Tatsuo Kanno, and Shih-Chieh Liang, and Pao-Yang Chen, and Wen-Wei Liao, and Wen-Dar Lin, and Antonius J M Matzke, and Marjori Matzke
Institute of Plant and Microbial Biology, Nangang District, Taipei 115, Taiwan.

Alternative splicing is prevalent in plants, but little is known about its regulation in the context of developmental and signaling pathways. We describe here a new factor that influences pre-messengerRNA (mRNA) splicing and is essential for embryonic development in Arabidopsis thaliana. This factor was retrieved in a genetic screen that identified mutants impaired in expression of an alternatively spliced GFP reporter gene. In addition to the known spliceosomal component PRP8, the screen recovered Arabidopsis RTF2 (AtRTF2), a previously uncharacterized, evolutionarily conserved protein containing a replication termination factor 2 (Rtf2) domain. A homozygous null mutation in AtRTF2 is embryo lethal, indicating that AtRTF2 is an essential protein. Quantitative RT-PCR demonstrated that impaired expression of GFP in atrtf2 and prp8 mutants is due to inefficient splicing of the GFP pre-mRNA. A genome-wide analysis using RNA sequencing indicated that 13-16% of total introns are retained to a significant degree in atrtf2 mutants. Considering these results and previous suggestions that Rtf2 represents an ubiquitin-related domain, we discuss the possible role of AtRTF2 in ubiquitin-based regulation of pre-mRNA splicing.

UI MeSH Term Description Entries
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D009154 Mutation Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations. Mutations
D010641 Phenotype The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment. Phenotypes
D011485 Protein Binding The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments. Plasma Protein Binding Capacity,Binding, Protein
D005816 Genetic Complementation Test A test used to determine whether or not complementation (compensation in the form of dominance) will occur in a cell with a given mutant phenotype when another mutant genome, encoding the same mutant phenotype, is introduced into that cell. Allelism Test,Cis Test,Cis-Trans Test,Complementation Test,Trans Test,Allelism Tests,Cis Tests,Cis Trans Test,Cis-Trans Tests,Complementation Test, Genetic,Complementation Tests,Complementation Tests, Genetic,Genetic Complementation Tests,Trans Tests
D005838 Genotype The genetic constitution of the individual, comprising the ALLELES present at each GENETIC LOCUS. Genogroup,Genogroups,Genotypes
D000429 Alcohol Oxidoreductases A subclass of enzymes which includes all dehydrogenases acting on primary and secondary alcohols as well as hemiacetals. They are further classified according to the acceptor which can be NAD+ or NADP+ (subclass 1.1.1), cytochrome (1.1.2), oxygen (1.1.3), quinone (1.1.5), or another acceptor (1.1.99). Carbonyl Reductase,Ketone Reductase,Carbonyl Reductases,Ketone Reductases,Oxidoreductases, Alcohol,Reductase, Carbonyl,Reductase, Ketone,Reductases, Carbonyl,Reductases, Ketone
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D012322 RNA Precursors RNA transcripts of the DNA that are in some unfinished stage of post-transcriptional processing (RNA PROCESSING, POST-TRANSCRIPTIONAL) required for function. RNA precursors may undergo several steps of RNA SPLICING during which the phosphodiester bonds at exon-intron boundaries are cleaved and the introns are excised. Consequently a new bond is formed between the ends of the exons. Resulting mature RNAs can then be used; for example, mature mRNA (RNA, MESSENGER) is used as a template for protein production. Precursor RNA,Primary RNA Transcript,RNA, Messenger, Precursors,RNA, Ribosomal, Precursors,RNA, Small Nuclear, Precursors,RNA, Transfer, Precursors,Pre-mRNA,Pre-rRNA,Pre-snRNA,Pre-tRNA,Primary Transcript, RNA,RNA Precursor,mRNA Precursor,rRNA Precursor,snRNA Precursor,tRNA Precursor,Pre mRNA,Pre rRNA,Pre snRNA,Pre tRNA,Precursor, RNA,Precursor, mRNA,Precursor, rRNA,Precursor, snRNA,Precursor, tRNA,Precursors, RNA,RNA Primary Transcript,RNA Transcript, Primary,RNA, Precursor,Transcript, Primary RNA,Transcript, RNA Primary
D015870 Gene Expression The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION. Expression, Gene,Expressions, Gene,Gene Expressions

Related Publications

Taku Sasaki, and Tatsuo Kanno, and Shih-Chieh Liang, and Pao-Yang Chen, and Wen-Wei Liao, and Wen-Dar Lin, and Antonius J M Matzke, and Marjori Matzke
May 2021, BMC plant biology,
Taku Sasaki, and Tatsuo Kanno, and Shih-Chieh Liang, and Pao-Yang Chen, and Wen-Wei Liao, and Wen-Dar Lin, and Antonius J M Matzke, and Marjori Matzke
February 1998, Molecular and cellular biology,
Taku Sasaki, and Tatsuo Kanno, and Shih-Chieh Liang, and Pao-Yang Chen, and Wen-Wei Liao, and Wen-Dar Lin, and Antonius J M Matzke, and Marjori Matzke
May 2019, The Plant journal : for cell and molecular biology,
Taku Sasaki, and Tatsuo Kanno, and Shih-Chieh Liang, and Pao-Yang Chen, and Wen-Wei Liao, and Wen-Dar Lin, and Antonius J M Matzke, and Marjori Matzke
May 2014, The Plant journal : for cell and molecular biology,
Taku Sasaki, and Tatsuo Kanno, and Shih-Chieh Liang, and Pao-Yang Chen, and Wen-Wei Liao, and Wen-Dar Lin, and Antonius J M Matzke, and Marjori Matzke
January 2019, PloS one,
Taku Sasaki, and Tatsuo Kanno, and Shih-Chieh Liang, and Pao-Yang Chen, and Wen-Wei Liao, and Wen-Dar Lin, and Antonius J M Matzke, and Marjori Matzke
September 2015, Nature communications,
Taku Sasaki, and Tatsuo Kanno, and Shih-Chieh Liang, and Pao-Yang Chen, and Wen-Wei Liao, and Wen-Dar Lin, and Antonius J M Matzke, and Marjori Matzke
September 2019, Plant & cell physiology,
Taku Sasaki, and Tatsuo Kanno, and Shih-Chieh Liang, and Pao-Yang Chen, and Wen-Wei Liao, and Wen-Dar Lin, and Antonius J M Matzke, and Marjori Matzke
February 2022, International journal of molecular sciences,
Taku Sasaki, and Tatsuo Kanno, and Shih-Chieh Liang, and Pao-Yang Chen, and Wen-Wei Liao, and Wen-Dar Lin, and Antonius J M Matzke, and Marjori Matzke
June 2020, G3 (Bethesda, Md.),
Taku Sasaki, and Tatsuo Kanno, and Shih-Chieh Liang, and Pao-Yang Chen, and Wen-Wei Liao, and Wen-Dar Lin, and Antonius J M Matzke, and Marjori Matzke
July 2002, The Journal of biological chemistry,
Copied contents to your clipboard!