-transparent.png){kind=logo}
We have examined the effect of histone acetylation on the in vitro assembly of nucleosomes with DNA and purified histones at physiological ionic strength in the presence of polyglutamic acid. We have found that hyperacetylated histones assemble nucleosomes with greater efficiency, and to a greater extent, than either control or hypoacetylated histones. Assembly reactions were performed over a range of histone to DNA ratios (0.25 to 3.0, w/w) and polyglutamic acid to histone ratios (0 to 1.6, w/w). Although polyglutamic acid may act as a sink to prevent nonspecific histone-DNA interactions, our data suggest that the polyanion primarily facilitates the assembly of nucleosomes by organizing histones into a form that is amenable to deposition.
M Cotten, and
R Chalkley
September 1980,
Nucleic acids research,
M Cotten, and
R Chalkley
June 1978,
Biochemical and biophysical research communications,
M Cotten, and
R Chalkley
May 1978,
Biochemical and biophysical research communications,
M Cotten, and
R Chalkley
April 1981,
Biochemistry,
M Cotten, and
R Chalkley
January 2005,
Annual review of cell and developmental biology,
M Cotten, and
R Chalkley
January 1999,
Methods in molecular biology (Clifton, N.J.),
M Cotten, and
R Chalkley
June 2005,
The Journal of biological chemistry,
M Cotten, and
R Chalkley
January 1994,
Biofizika,
M Cotten, and
R Chalkley
November 1984,
Experimental cell research,
M Cotten, and
R Chalkley
February 2000,
FEBS letters,
