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To analyze transcription in Typanosoma brucei, we have characterized the trypanosomal RNA polymerases. Here we present our results, which allow a discrimination between the different classes of RNA polymerases in nuclear run-on experiments by polymerase inhibitors and Mn2+ dependence. We also describe the separation of trypanosomal RNA polymerases by chromatography, demonstrating that T. brucei contains RNA polymerases I-III. The outcome of our experiments suggests that the VSG genes of T. brucei are not transcribed by RNA polymerase I, as previously reported, but by RNA polymerase II. We propose that an additional factor modifies RNA polymerase II, resulting in the alpha-amanitin-resistant transcription of VSG genes. Our data also suggest that the mini-exon genes, which encode the 5' end of each trypanosomal mRNA, are probably transcribed by RNA polymerase III.
E J Grondal, and
R Evers, and
K Kosubek, and
A W Cornelissen
December 1989,
The EMBO journal,
E J Grondal, and
R Evers, and
K Kosubek, and
A W Cornelissen
December 2006,
Molecular and biochemical parasitology,
E J Grondal, and
R Evers, and
K Kosubek, and
A W Cornelissen
July 2006,
Molecular and biochemical parasitology,
E J Grondal, and
R Evers, and
K Kosubek, and
A W Cornelissen
October 1989,
The Journal of biological chemistry,
E J Grondal, and
R Evers, and
K Kosubek, and
A W Cornelissen
December 2009,
Experimental parasitology,
E J Grondal, and
R Evers, and
K Kosubek, and
A W Cornelissen
January 2014,
PloS one,
E J Grondal, and
R Evers, and
K Kosubek, and
A W Cornelissen
June 2003,
Molecular and biochemical parasitology,
E J Grondal, and
R Evers, and
K Kosubek, and
A W Cornelissen
January 1979,
Biochimica et biophysica acta,
E J Grondal, and
R Evers, and
K Kosubek, and
A W Cornelissen
May 2011,
The EMBO journal,
E J Grondal, and
R Evers, and
K Kosubek, and
A W Cornelissen
February 2005,
Molecular and biochemical parasitology,
