Biconically tapered fiber optic probes for rapid label-free immunoassays. 2015

John Miller, and Angelica Castaneda, and Kun Ho Lee, and Martin Sanchez, and Adrian Ortiz, and Ekrem Almaz, and Zuleyha Turkoglu Almaz, and Shelton Murinda, and Wei-Jen Lin, and Ertan Salik
Department of Physics and Astronomy, University of California, Los Angeles, 475 Portola Plaza, Los Angeles, CA 90095, USA. johnmiller@physics.ucla.edu.

We report use of U-shaped biconically tapered optical fibers (BTOF) as probes for label-free immunoassays. The tapered regions of the sensors were functionalized by immobilization of immunoglobulin-G (Ig-G) and tested for detection of anti-IgG at concentrations of 50 ng/mL to 50 µg/mL. Antibody-antigen reaction creates a biological nanolayer modifying the waveguide structure leading to a change in the sensor signal, which allows real-time monitoring. The kinetics of the antibody (mouse Ig-G)-antigen (rabbit anti-mouse IgG) reactions was studied. Hydrofluoric acid treatment makes the sensitive region thinner to enhance sensitivity, which we confirmed by experiments and simulations. The limit of detection for the sensor was estimated to be less than 50 ng/mL. Utilization of the rate of the sensor peak shift within the first few minutes of the antibody-antigen reaction is proposed as a rapid protein detection method.

UI MeSH Term Description Entries
D007074 Immunoglobulin G The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B. Gamma Globulin, 7S,IgG,IgG Antibody,Allerglobuline,IgG(T),IgG1,IgG2,IgG2A,IgG2B,IgG3,IgG4,Immunoglobulin GT,Polyglobin,7S Gamma Globulin,Antibody, IgG,GT, Immunoglobulin
D007118 Immunoassay A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody production and in measurement of antibody by the test substance. Immunochromatographic Assay,Assay, Immunochromatographic,Assays, Immunochromatographic,Immunoassays,Immunochromatographic Assays
D007700 Kinetics The rate dynamics in chemical or physical systems.
D011817 Rabbits A burrowing plant-eating mammal with hind limbs that are longer than its fore limbs. It belongs to the family Leporidae of the order Lagomorpha, and in contrast to hares, possesses 22 instead of 24 pairs of chromosomes. Belgian Hare,New Zealand Rabbit,New Zealand Rabbits,New Zealand White Rabbit,Rabbit,Rabbit, Domestic,Chinchilla Rabbits,NZW Rabbits,New Zealand White Rabbits,Oryctolagus cuniculus,Chinchilla Rabbit,Domestic Rabbit,Domestic Rabbits,Hare, Belgian,NZW Rabbit,Rabbit, Chinchilla,Rabbit, NZW,Rabbit, New Zealand,Rabbits, Chinchilla,Rabbits, Domestic,Rabbits, NZW,Rabbits, New Zealand,Zealand Rabbit, New,Zealand Rabbits, New,cuniculus, Oryctolagus
D006820 Hyaluronic Acid A natural high-viscosity mucopolysaccharide with alternating beta (1-3) glucuronide and beta (1-4) glucosaminidic bonds. It is found in the UMBILICAL CORD, in VITREOUS BODY and in SYNOVIAL FLUID. A high urinary level is found in PROGERIA. Amo Vitrax,Amvisc,Biolon,Etamucine,Healon,Hyaluronan,Hyaluronate Sodium,Hyvisc,Luronit,Sodium Hyaluronate,Acid, Hyaluronic,Hyaluronate, Sodium,Vitrax, Amo
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000888 Antibodies, Anti-Idiotypic Antibodies which react with the individual structural determinants (idiotopes) on the variable region of other antibodies. Anti-Antibodies,Anti-Idiotype Antibodies,Antibodies, Internal Image,Antigamma Globulin Antibodies,Antiglobulins,Anti Antibodies,Anti-gamma Globulin Antibodies,Anti Idiotype Antibodies,Anti gamma Globulin Antibodies,Anti-Idiotypic Antibodies,Antibodies, Anti,Antibodies, Anti Idiotypic,Antibodies, Anti-Idiotype,Antibodies, Anti-gamma Globulin,Antibodies, Antigamma Globulin,Globulin Antibodies, Anti-gamma,Globulin Antibodies, Antigamma,Image Antibodies, Internal,Internal Image Antibodies
D000937 Antigen-Antibody Reactions The processes triggered by interactions of ANTIBODIES with their ANTIGENS. Antigen Antibody Reactions,Antigen-Antibody Reaction,Reaction, Antigen-Antibody,Reactions, Antigen-Antibody
D015374 Biosensing Techniques Any of a variety of procedures which use biomolecular probes to measure the presence or concentration of biological molecules, biological structures, microorganisms, etc., by translating a biochemical interaction at the probe surface into a quantifiable physical signal. Bioprobes,Biosensors,Electrodes, Enzyme,Biosensing Technics,Bioprobe,Biosensing Technic,Biosensing Technique,Biosensor,Electrode, Enzyme,Enzyme Electrode,Enzyme Electrodes,Technic, Biosensing,Technics, Biosensing,Technique, Biosensing,Techniques, Biosensing
D051379 Mice The common name for the genus Mus. Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus

Related Publications

John Miller, and Angelica Castaneda, and Kun Ho Lee, and Martin Sanchez, and Adrian Ortiz, and Ekrem Almaz, and Zuleyha Turkoglu Almaz, and Shelton Murinda, and Wei-Jen Lin, and Ertan Salik
July 2021, Analytica chimica acta,
John Miller, and Angelica Castaneda, and Kun Ho Lee, and Martin Sanchez, and Adrian Ortiz, and Ekrem Almaz, and Zuleyha Turkoglu Almaz, and Shelton Murinda, and Wei-Jen Lin, and Ertan Salik
January 2011, Sensors (Basel, Switzerland),
John Miller, and Angelica Castaneda, and Kun Ho Lee, and Martin Sanchez, and Adrian Ortiz, and Ekrem Almaz, and Zuleyha Turkoglu Almaz, and Shelton Murinda, and Wei-Jen Lin, and Ertan Salik
January 2010, Proceedings of SPIE--the International Society for Optical Engineering,
John Miller, and Angelica Castaneda, and Kun Ho Lee, and Martin Sanchez, and Adrian Ortiz, and Ekrem Almaz, and Zuleyha Turkoglu Almaz, and Shelton Murinda, and Wei-Jen Lin, and Ertan Salik
April 2013, The Analyst,
John Miller, and Angelica Castaneda, and Kun Ho Lee, and Martin Sanchez, and Adrian Ortiz, and Ekrem Almaz, and Zuleyha Turkoglu Almaz, and Shelton Murinda, and Wei-Jen Lin, and Ertan Salik
February 1988, Talanta,
John Miller, and Angelica Castaneda, and Kun Ho Lee, and Martin Sanchez, and Adrian Ortiz, and Ekrem Almaz, and Zuleyha Turkoglu Almaz, and Shelton Murinda, and Wei-Jen Lin, and Ertan Salik
June 1994, IEEE transactions on bio-medical engineering,
John Miller, and Angelica Castaneda, and Kun Ho Lee, and Martin Sanchez, and Adrian Ortiz, and Ekrem Almaz, and Zuleyha Turkoglu Almaz, and Shelton Murinda, and Wei-Jen Lin, and Ertan Salik
February 2010, Proceedings of SPIE--the International Society for Optical Engineering,
John Miller, and Angelica Castaneda, and Kun Ho Lee, and Martin Sanchez, and Adrian Ortiz, and Ekrem Almaz, and Zuleyha Turkoglu Almaz, and Shelton Murinda, and Wei-Jen Lin, and Ertan Salik
January 2003, Journal of biomedical optics,
John Miller, and Angelica Castaneda, and Kun Ho Lee, and Martin Sanchez, and Adrian Ortiz, and Ekrem Almaz, and Zuleyha Turkoglu Almaz, and Shelton Murinda, and Wei-Jen Lin, and Ertan Salik
June 1995, Fiziologicheskii zhurnal imeni I.M. Sechenova,
John Miller, and Angelica Castaneda, and Kun Ho Lee, and Martin Sanchez, and Adrian Ortiz, and Ekrem Almaz, and Zuleyha Turkoglu Almaz, and Shelton Murinda, and Wei-Jen Lin, and Ertan Salik
November 2014, Biosensors & bioelectronics,
Copied contents to your clipboard!