OBJECTIVE To compare the effect of combinations of fluoride (F) products on remineralization of caries-like lesions. METHODS Demineralized human enamel specimens were assessed by surface microhardness (SMH), stratified and allocated to five treatments groups: (1) Toothpaste containing 1% nano hydroxyapatite (HAP) + 1,450 ppm F; (2) Tooth mousse containing 10% casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) + 900 ppm F plus F toothpaste (1,450 ppm F as NaF); (3) F varnish containing 22,600 ppm F plus F toothpaste (1,450 ppm F as NaF); (4) F toothpaste (1,450 ppm F as NaF); and (5) placebo--distilled water (P). Each group was treated in a 21-day pH-cycling model. Groups 1, 4 and 5 were treated 2x/day. In Group 3, the tooth mousse was administered 1x/day plus 2x/day F toothpaste treatment. In Group 4, F varnish was administered 1x/week plus 2x/day treatment with F toothpaste. After cycling, SMH was re-measured and cross-sectional microhardness measurements were taken. RESULTS Groups 1-4 produced significant rehardening of enamel. In the superficial layer (25-50 μm deep) significantly higher mineralization (up to 83%, by Volume (V), P < 0.0001) was achieved for Groups 2 and 3. In the area of 75-100 μm deep, the highest mineralization was observed for Groups 1 and 4 (V% = 68-83%).