BIOMAT Database Logo BIOMAT Database Logo

Light-mediated DNA Repair Prevents UVB-induced Cell Cycle Arrest in Embryos of the Crustacean Macrobrachium olfersi. 2015

Eliane Cristina Zeni, and Dib Ammar, and Mayana Lacerda Leal, and Heloisa Schramm da Silva, and Silvana Allodi, and Yara Maria Rauh Müller, and Evelise Maria Nazari
Departamento de Biologia Celular, Embriologia e Genética, Universidade Federal de Santa Catarina, Florianópolis, Santa Catarina, Brazil.

High levels of ultraviolet-B (UVB) radiation can negatively affect aquatic animals. Macrobrachium olfersi is a prawn that lives in clear freshwaters and during the breeding season, females carry eggs in an external brood pouch. Therefore, we hypothesize that eggs are also exposed to environmental UVB radiation. The aim of this study was to investigate whether UVB radiation induces DNA damage and compromises cell cycle in embryos of M. olfersi. In laboratory, UVB irradiance (310 mW. cm(-2) ) that embryos receive in the natural environment was simulated. After irradiation, embryos were kept under different light conditions in order to recognize the presence of cell repair. UVB radiation induces DNA damage, specifically thymine dimers. After 48 h of UVB exposure, a significant decrease in the level of these dimers was observed in embryos kept under visible light while it remained constant in the dark. Moreover, under visible light and darkness, a decrease in proliferation was observed after 48 h of irradiation. An increase in PCNA expression and decrease in p53 expression were observed after, respectively, 1 and 48 h of exposure. Our results showed that UVB radiation disturbs the cell cycle and induces DNA damage in M. olfersi embryos. However, under visible light these embryos showed successful DNA repair.

UI MeSH Term Description Entries
D002453 Cell Cycle The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE. Cell Division Cycle,Cell Cycles,Cell Division Cycles,Cycle, Cell,Cycle, Cell Division,Cycles, Cell,Cycles, Cell Division,Division Cycle, Cell,Division Cycles, Cell
D003445 Crustacea A large subphylum of mostly marine ARTHROPODS containing over 42,000 species. They include familiar arthropods such as lobsters (NEPHROPIDAE), crabs (BRACHYURA), shrimp (PENAEIDAE), and barnacles (THORACICA). Ostracoda,Ostracods,Crustaceas,Ostracod,Ostracodas
D004260 DNA Repair The removal of DNA LESIONS and/or restoration of intact DNA strands without BASE PAIR MISMATCHES, intrastrand or interstrand crosslinks, or discontinuities in the DNA sugar-phosphate backbones. DNA Damage Response
D005260 Female Females
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D014466 Ultraviolet Rays That portion of the electromagnetic spectrum immediately below the visible range and extending into the x-ray frequencies. The longer wavelengths (near-UV or biotic or vital rays) are necessary for the endogenous synthesis of vitamin D and are also called antirachitic rays; the shorter, ionizing wavelengths (far-UV or abiotic or extravital rays) are viricidal, bactericidal, mutagenic, and carcinogenic and are used as disinfectants. Actinic Rays,Black Light, Ultraviolet,UV Light,UV Radiation,Ultra-Violet Rays,Ultraviolet Light,Ultraviolet Radiation,Actinic Ray,Light, UV,Light, Ultraviolet,Radiation, UV,Radiation, Ultraviolet,Ray, Actinic,Ray, Ultra-Violet,Ray, Ultraviolet,Ultra Violet Rays,Ultra-Violet Ray,Ultraviolet Black Light,Ultraviolet Black Lights,Ultraviolet Radiations,Ultraviolet Ray

Related Publications

Eliane Cristina Zeni, and Dib Ammar, and Mayana Lacerda Leal, and Heloisa Schramm da Silva, and Silvana Allodi, and Yara Maria Rauh Müller, and Evelise Maria Nazari
Effects of environmental and artificial UV-B radiation on freshwater prawn Macrobrachium olfersi embryos.
June 2010, Aquatic toxicology (Amsterdam, Netherlands),
Eliane Cristina Zeni, and Dib Ammar, and Mayana Lacerda Leal, and Heloisa Schramm da Silva, and Silvana Allodi, and Yara Maria Rauh Müller, and Evelise Maria Nazari
Mismatch repair protein Msh2 contributes to UVB-induced cell cycle arrest in epidermal and cultured mouse keratinocytes.
January 2005, DNA repair,
Eliane Cristina Zeni, and Dib Ammar, and Mayana Lacerda Leal, and Heloisa Schramm da Silva, and Silvana Allodi, and Yara Maria Rauh Müller, and Evelise Maria Nazari
Nek1 silencing slows down DNA repair and blocks DNA damage-induced cell cycle arrest.
September 2010, Mutagenesis,
Eliane Cristina Zeni, and Dib Ammar, and Mayana Lacerda Leal, and Heloisa Schramm da Silva, and Silvana Allodi, and Yara Maria Rauh Müller, and Evelise Maria Nazari
Cell cycle arrest mediated by Cd-induced DNA damage in Arabidopsis root tips.
November 2017, Ecotoxicology and environmental safety,
Eliane Cristina Zeni, and Dib Ammar, and Mayana Lacerda Leal, and Heloisa Schramm da Silva, and Silvana Allodi, and Yara Maria Rauh Müller, and Evelise Maria Nazari
Benzo[a]pyrene induced p53-mediated cell cycle arrest, DNA repair, and apoptosis pathways in Chinese rare minnow (Gobiocypris rarus).
March 2017, Environmental toxicology,
Eliane Cristina Zeni, and Dib Ammar, and Mayana Lacerda Leal, and Heloisa Schramm da Silva, and Silvana Allodi, and Yara Maria Rauh Müller, and Evelise Maria Nazari
Dichlorvos-induced cell cycle arrest and DNA damage repair activation in primary rat microglial cells.
March 2013, Journal of neuroscience research,
Eliane Cristina Zeni, and Dib Ammar, and Mayana Lacerda Leal, and Heloisa Schramm da Silva, and Silvana Allodi, and Yara Maria Rauh Müller, and Evelise Maria Nazari
Induced Endothelial Cell Cycle Arrest Prevents Arteriovenous Malformations in Hereditary Hemorrhagic Telangiectasia.
March 2024, Circulation,
Eliane Cristina Zeni, and Dib Ammar, and Mayana Lacerda Leal, and Heloisa Schramm da Silva, and Silvana Allodi, and Yara Maria Rauh Müller, and Evelise Maria Nazari
DNA damage and p53-mediated cell cycle arrest: a reevaluation.
December 1996, Proceedings of the National Academy of Sciences of the United States of America,
Eliane Cristina Zeni, and Dib Ammar, and Mayana Lacerda Leal, and Heloisa Schramm da Silva, and Silvana Allodi, and Yara Maria Rauh Müller, and Evelise Maria Nazari
p53-independent DNA repair and cell cycle arrest in embryonic stem cells.
April 1998, FEBS letters,
Eliane Cristina Zeni, and Dib Ammar, and Mayana Lacerda Leal, and Heloisa Schramm da Silva, and Silvana Allodi, and Yara Maria Rauh Müller, and Evelise Maria Nazari
The DNA-mismatch repair enzyme hMSH2 modulates UV-B-induced cell cycle arrest and apoptosis in melanoma cells.
January 2008, The Journal of investigative dermatology,
Copied contents to your clipboard!