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A strain (MPUAT-2), isolated from coconut hull and identified as Aspergillus foetidus MTCC 10559, was used for pectinase production. Optimum pectinase production was obtained at pH 8.0 and temperature 35 degrees C under static conditions in submerged fermentation after 5 days of incubation. Orange peel, a byproduct of fruit industry, was used as a sole carbon source (3% w/v) to produce high pectinase, thus making the process cost effective. The culture filtrate was analyzed for pectin methyl esterase (PME) and endopolygalacturonase (endo-PG) enzymes. The enzymes, PME and endo-PG were purified using ammonium sulphate precipitation and molecular exclusion chromatography (Sephadex G-75) with corresponding recovery of 39.3 and 44.3%. The partially purified enzymes were also characterized for their kinetic properties.
Sunil Kumar, and
Narender K Jain, and
Kailash C Sharma, and
Ranjeet Paswan, and
Brijesh K Mishra, and
Ramakrishnan Srinivasan, and
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AMB Express,
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Narender K Jain, and
Kailash C Sharma, and
Ranjeet Paswan, and
Brijesh K Mishra, and
Ramakrishnan Srinivasan, and
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Sunil Kumar, and
Narender K Jain, and
Kailash C Sharma, and
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Ramakrishnan Srinivasan, and
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Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association,
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Narender K Jain, and
Kailash C Sharma, and
Ranjeet Paswan, and
Brijesh K Mishra, and
Ramakrishnan Srinivasan, and
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Indian journal of biochemistry & biophysics,
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Ramakrishnan Srinivasan, and
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Journal of basic microbiology,
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Biotechnology progress,
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Enzyme research,
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May 2021,
Applied biochemistry and biotechnology,
Sunil Kumar, and
Narender K Jain, and
Kailash C Sharma, and
Ranjeet Paswan, and
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Shiwani Mandhania
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Applied biochemistry and biotechnology,
Sunil Kumar, and
Narender K Jain, and
Kailash C Sharma, and
Ranjeet Paswan, and
Brijesh K Mishra, and
Ramakrishnan Srinivasan, and
Shiwani Mandhania
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Prikladnaia biokhimiia i mikrobiologiia,
