BIOMAT Database Logo BIOMAT Database Logo

Role for T cells, IL-2 and IL-6 in the IL-4-dependent in vitro human IgE synthesis. 1989

E Maggi, and G F Del Prete, and P Parronchi, and A Tiri, and D Macchia, and P Biswas, and C Simonelli, and M Ricci, and S Romagnani
Division of Allergology and Clinical Immunology, University of Florence, Italy.

The role of T cells and monocytes, as well as that of cytokines, such as IL-1, IL-2 and IL-6, on the IL-4-dependent in vitro human IgE synthesis was investigated. Recombinant IL-4, IL-4-containing T-cell clone supernatants and different combinations of recombinant cytokines failed to induce highly purified B cells to synthesize IgE. IL-4-dependent IgE synthesis was restored by addition to purified B cells of either untreated or mitomycin C-treated autologous T lymphocytes. Addition to purified B cells of autologous monocytes did not restore the IgE response, but usually it exerted a potentiating effect on the synthesis of IgE induced by IL-4 in the presence of suboptimal concentrations of T cells. The activity of T cells apparently preceded that of IL-4 and required a physical contact with B cells. The presence in culture of IL-2 also appeared to be necessary for the T-cell and IL-4-dependent IgE synthesis. Even though not essential, IL-6 was able to potentiate IgE synthesis in most experiments, whereas IL-1 did not display any modulatory effect.

UI MeSH Term Description Entries
D007073 Immunoglobulin E An immunoglobulin associated with MAST CELLS. Overexpression has been associated with allergic hypersensitivity (HYPERSENSITIVITY, IMMEDIATE). IgE
D007376 Interleukin-2 A soluble substance elaborated by antigen- or mitogen-stimulated T-LYMPHOCYTES which induces DNA synthesis in naive lymphocytes. IL-2,Lymphocyte Mitogenic Factor,T-Cell Growth Factor,TCGF,IL2,Interleukin II,Interleukine 2,RU 49637,RU-49637,Ro-23-6019,Ro-236019,T-Cell Stimulating Factor,Thymocyte Stimulating Factor,Interleukin 2,Mitogenic Factor, Lymphocyte,RU49637,Ro 23 6019,Ro 236019,Ro236019,T Cell Growth Factor,T Cell Stimulating Factor
D009000 Monocytes Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles. Monocyte
D011994 Recombinant Proteins Proteins prepared by recombinant DNA technology. Biosynthetic Protein,Biosynthetic Proteins,DNA Recombinant Proteins,Recombinant Protein,Proteins, Biosynthetic,Proteins, Recombinant DNA,DNA Proteins, Recombinant,Protein, Biosynthetic,Protein, Recombinant,Proteins, DNA Recombinant,Proteins, Recombinant,Recombinant DNA Proteins,Recombinant Proteins, DNA
D002450 Cell Communication Any of several ways in which living cells of an organism communicate with one another, whether by direct contact between cells or by means of chemical signals carried by neurotransmitter substances, hormones, and cyclic AMP. Cell Interaction,Cell-to-Cell Interaction,Cell Communications,Cell Interactions,Cell to Cell Interaction,Cell-to-Cell Interactions,Communication, Cell,Communications, Cell,Interaction, Cell,Interaction, Cell-to-Cell,Interactions, Cell,Interactions, Cell-to-Cell
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001402 B-Lymphocytes Lymphoid cells concerned with humoral immunity. They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation. B-Cells, Lymphocyte,B-Lymphocyte,Bursa-Dependent Lymphocytes,B Cells, Lymphocyte,B Lymphocyte,B Lymphocytes,B-Cell, Lymphocyte,Bursa Dependent Lymphocytes,Bursa-Dependent Lymphocyte,Lymphocyte B-Cell,Lymphocyte B-Cells,Lymphocyte, Bursa-Dependent,Lymphocytes, Bursa-Dependent
D013601 T-Lymphocytes Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen. T Cell,T Lymphocyte,T-Cells,Thymus-Dependent Lymphocytes,Cell, T,Cells, T,Lymphocyte, T,Lymphocyte, Thymus-Dependent,Lymphocytes, T,Lymphocytes, Thymus-Dependent,T Cells,T Lymphocytes,T-Cell,T-Lymphocyte,Thymus Dependent Lymphocytes,Thymus-Dependent Lymphocyte
D015847 Interleukin-4 A soluble factor produced by activated T-LYMPHOCYTES that induces the expression of MHC CLASS II GENES and FC RECEPTORS on B-LYMPHOCYTES and causes their proliferation and differentiation. It also acts on T-lymphocytes, MAST CELLS, and several other hematopoietic lineage cells. B-Cell Growth Factor-I,B-Cell Stimulatory Factor-1,Binetrakin,IL-4,Mast Cell Growth Factor-2,B Cell Stimulatory Factor-1,B-Cell Growth Factor-1,B-Cell Proliferating Factor,B-Cell Stimulating Factor-1,B-Cell Stimulatory Factor 1,BCGF-1,BSF-1,IL4,MCGF-2,B Cell Growth Factor 1,B Cell Growth Factor I,B Cell Proliferating Factor,B Cell Stimulating Factor 1,B Cell Stimulatory Factor 1,Interleukin 4,Mast Cell Growth Factor 2

Related Publications

E Maggi, and G F Del Prete, and P Parronchi, and A Tiri, and D Macchia, and P Biswas, and C Simonelli, and M Ricci, and S Romagnani
Role of IL-6, IL-2, and IL-4 in the in vitro induction of cytotoxic T cells.
November 1992, Journal of immunology (Baltimore, Md. : 1950),
E Maggi, and G F Del Prete, and P Parronchi, and A Tiri, and D Macchia, and P Biswas, and C Simonelli, and M Ricci, and S Romagnani
Immunostimulatory DNA inhibits IL-4-dependent IgE synthesis by human B cells.
September 2001, The Journal of allergy and clinical immunology,
E Maggi, and G F Del Prete, and P Parronchi, and A Tiri, and D Macchia, and P Biswas, and C Simonelli, and M Ricci, and S Romagnani
Regulation of BSF-2/IL-6 production by human mononuclear cells. Macrophage-dependent synthesis of BSF-2/IL-6 by T cells.
September 1988, Journal of immunology (Baltimore, Md. : 1950),
E Maggi, and G F Del Prete, and P Parronchi, and A Tiri, and D Macchia, and P Biswas, and C Simonelli, and M Ricci, and S Romagnani
IL-2 inhibits IL-4-dependent IgE and IgG1 production in vitro and in vivo.
February 1995, International immunology,
E Maggi, and G F Del Prete, and P Parronchi, and A Tiri, and D Macchia, and P Biswas, and C Simonelli, and M Ricci, and S Romagnani
IL-4 inhibits IL-2 receptor expression and IL-2-dependent proliferation of human T cells.
March 1990, Journal of immunology (Baltimore, Md. : 1950),
E Maggi, and G F Del Prete, and P Parronchi, and A Tiri, and D Macchia, and P Biswas, and C Simonelli, and M Ricci, and S Romagnani
Influence of IL-2 and IL-4 on the IgE synthesis and the IgE-binding factor (sCD23) production by human lymphocytes in vitro.
March 1989, Immunology,
E Maggi, and G F Del Prete, and P Parronchi, and A Tiri, and D Macchia, and P Biswas, and C Simonelli, and M Ricci, and S Romagnani
Endogenous interleukin 6 plays an obligatory role in interleukin 4-dependent human IgE synthesis.
August 1989, European journal of immunology,
E Maggi, and G F Del Prete, and P Parronchi, and A Tiri, and D Macchia, and P Biswas, and C Simonelli, and M Ricci, and S Romagnani
IgE-dependent expression of mRNA for IL-4 and IL-5 in human lung mast cells.
August 1995, Journal of immunology (Baltimore, Md. : 1950),
E Maggi, and G F Del Prete, and P Parronchi, and A Tiri, and D Macchia, and P Biswas, and C Simonelli, and M Ricci, and S Romagnani
IL-4-dependent IgE switch in membrane IgA-positive human B cells.
November 1991, Journal of immunology (Baltimore, Md. : 1950),
E Maggi, and G F Del Prete, and P Parronchi, and A Tiri, and D Macchia, and P Biswas, and C Simonelli, and M Ricci, and S Romagnani
The relative contribution of IL-4 and IL-13 to human IgE synthesis induced by activated CD4+ or CD8+ T cells.
December 1997, The Journal of allergy and clinical immunology,
Copied contents to your clipboard!