Biomaterial Database

Blocking of cytotoxic T-cell function of MOLF/Ei mice by anti-Ly 35.1 monoclonal antibody. 1989

M Mieno, and H Udono, and S Ikegami, and N Tada, and H Shiku, and E Nakayama

Department of Immunology, Nagasaki University School of Medicine, Japan.

Ly 35.1 antigen is an alloantigen expressed only on T cells of Mus musculus molossinus-derived inbred strains. Previous findings indicated that the genetic locus coding for Ly 35 antigen was closely linked to Ly 2/3 on chromosome 6 and that epitopes detected by Ly 2.1 and Ly 35.1 monoclonal antibodies (mAb) were closely associated, as shown by binding inhibition assay. In this study, we examined the blocking effects of anti-Ly 2.1 and anti-Ly 35.1 mAb on cytotoxic T-cell function of MOLF/Ei mice generated against BALB/c. MOLF/Ei cytotoxicity was blocked by Ly 35.1 mAb, but not by anti-Ly 2.1 mAb. Additional tests showed that cytotoxicity was blocked by Ly 3.1 mAb, but not Ly 3.2 or Ly 2.2 mAb. These results suggested that MOLF/Ei mice express Ly 3.1, but not Ly 2.1 antigen at a functional level, and that Ly 35.1 may be a functional epitope of Ly 2 antigen in the MOLF/Ei strain.

UI	MeSH Term	Description	Entries
D007519	Isoantigens	Antigens that exist in alternative (allelic) forms in a single species. When an isoantigen is encountered by species members who lack it, an immune response is induced. Typical isoantigens are the BLOOD GROUP ANTIGENS.	Alloantigens,Alloantigen,Isoantigen
D008815	Mice, Inbred Strains	Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.	Inbred Mouse Strains,Inbred Strain of Mice,Inbred Strain of Mouse,Inbred Strains of Mice,Mouse, Inbred Strain,Inbred Mouse Strain,Mouse Inbred Strain,Mouse Inbred Strains,Mouse Strain, Inbred,Mouse Strains, Inbred,Strain, Inbred Mouse,Strains, Inbred Mouse
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D000911	Antibodies, Monoclonal	Antibodies produced by a single clone of cells.	Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal
D000950	Antigens, Ly	A group of lymphocyte surface antigens located on mouse LYMPHOCYTES. Specific Ly antigens are useful markers for distinguishing subpopulations of lymphocytes.	Ly Antigens
D013602	T-Lymphocytes, Cytotoxic	Immunized T-lymphocytes which can directly destroy appropriate target cells. These cytotoxic lymphocytes may be generated in vitro in mixed lymphocyte cultures (MLC), in vivo during a graft-versus-host (GVH) reaction, or after immunization with an allograft, tumor cell or virally transformed or chemically modified target cell. The lytic phenomenon is sometimes referred to as cell-mediated lympholysis (CML). These CD8-positive cells are distinct from NATURAL KILLER CELLS and NATURAL KILLER T-CELLS. There are two effector phenotypes: TC1 and TC2.	Cell-Mediated Lympholytic Cells,Cytotoxic T Cells,Cytotoxic T Lymphocyte,Cytotoxic T-Lymphocytes,TC1 Cell,TC1 Cells,TC2 Cell,TC2 Cells,Cell Mediated Lympholytic Cells,Cell, Cell-Mediated Lympholytic,Cell, TC1,Cell, TC2,Cell-Mediated Lympholytic Cell,Cytotoxic T Cell,Cytotoxic T Lymphocytes,Cytotoxic T-Lymphocyte,Lymphocyte, Cytotoxic T,Lympholytic Cell, Cell-Mediated,Lympholytic Cells, Cell-Mediated,T Cell, Cytotoxic,T Lymphocyte, Cytotoxic,T Lymphocytes, Cytotoxic,T-Lymphocyte, Cytotoxic
D051379	Mice	The common name for the genus Mus.	Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus