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We developed a simple, sensitive laser nephelometric assay (LNA) to detect circulating staphylococcal enterotoxin B (SEB). This assay yields the result within 2 h and needs no special treatment. Influence of protein A, a product generated by Staphylococcus aureus, was negligible in this assay. The levels of SEB in plasma were measured in 28 patients with and without S. aureus infection with the LNA. The levels of SEB in plasma increased significantly in patients with S. aureus infection. We also demonstrated that the levels of SEB in plasma were higher in patients with methicillin-resistant S. aureus infection than in patients with nonresistant staphylococcal infection. Our data indicate that LNA is a useful assay in the early diagnosis of methicillin-resistant S. aureus infection.
K K Hosotsubo, and
H Hosotsubo, and
M K Nishijima, and
M Nishimura, and
N Taenaka, and
I Yoshiya
May 2021,
The Journal of infectious diseases,
K K Hosotsubo, and
H Hosotsubo, and
M K Nishijima, and
M Nishimura, and
N Taenaka, and
I Yoshiya
February 1976,
Canadian journal of microbiology,
K K Hosotsubo, and
H Hosotsubo, and
M K Nishijima, and
M Nishimura, and
N Taenaka, and
I Yoshiya
October 1968,
Journal of bacteriology,
K K Hosotsubo, and
H Hosotsubo, and
M K Nishijima, and
M Nishimura, and
N Taenaka, and
I Yoshiya
September 1999,
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America,
K K Hosotsubo, and
H Hosotsubo, and
M K Nishijima, and
M Nishimura, and
N Taenaka, and
I Yoshiya
January 1972,
Biochimica et biophysica acta,
K K Hosotsubo, and
H Hosotsubo, and
M K Nishijima, and
M Nishimura, and
N Taenaka, and
I Yoshiya
February 1969,
Journal of bacteriology,
K K Hosotsubo, and
H Hosotsubo, and
M K Nishijima, and
M Nishimura, and
N Taenaka, and
I Yoshiya
April 1997,
International journal of food microbiology,
K K Hosotsubo, and
H Hosotsubo, and
M K Nishijima, and
M Nishimura, and
N Taenaka, and
I Yoshiya
August 2005,
Journal of bacteriology,
K K Hosotsubo, and
H Hosotsubo, and
M K Nishijima, and
M Nishimura, and
N Taenaka, and
I Yoshiya
May 1988,
The Journal of biological chemistry,
K K Hosotsubo, and
H Hosotsubo, and
M K Nishijima, and
M Nishimura, and
N Taenaka, and
I Yoshiya
January 2021,
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology,
