Biomaterial Database

Effect of copper loading of preruminant calves on intracellular distribution of hepatic copper, zinc, iron, and molybdenum. 1989

K J Jenkins

Animal Research Centre, Agriculture Canada, Ottawa, Ontario.

The subcellular distribution of Cu, Zn, Fe, and Mo was investigated in liver homogenates from preruminant calves fed control Cu (10 ppm), high Cu (1000 ppm), or high Cu plus high Zn (1000 ppm) milk replacer. For controls, Cu was located primarily in the nuclei and large granule fractions, Zn mainly in the cytosol, and Fe in the cytosol and nuclei fraction; Mo was present in all compartments but least in microsomes. Calves fed high Cu had markedly increased hepatic Cu concentration in the nuclei and cytosol fractions, reduced cytosol Zn, increased nuclei Fe, and decreased Mo concentration in all cell compartments. Feeding high Zn with high Cu (which prevented deaths from high Cu) reversed some changes in hepatic trace element patterns caused by high Cu while initiating new alterations. The marked increase in hepatic Cu and reduced Mo in nuclei and cytosol after Cu loading indicate that these compartments may have a predominant role in the development of Cu toxicity in the preruminant calf.

UI	MeSH Term	Description	Entries
D007501	Iron	A metallic element with atomic symbol Fe, atomic number 26, and atomic weight 55.85. It is an essential constituent of HEMOGLOBINS; CYTOCHROMES; and IRON-BINDING PROTEINS. It plays a role in cellular redox reactions and in the transport of OXYGEN.	Iron-56,Iron 56
D008099	Liver	A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.	Livers
D008297	Male		Males
D008861	Microsomes	Artifactual vesicles formed from the endoplasmic reticulum when cells are disrupted. They are isolated by differential centrifugation and are composed of three structural features: rough vesicles, smooth vesicles, and ribosomes. Numerous enzyme activities are associated with the microsomal fraction. (Glick, Glossary of Biochemistry and Molecular Biology, 1990; from Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)	Microsome
D008982	Molybdenum	A metallic element with the atomic symbol Mo, atomic number 42, and atomic weight 95.95. It is an essential trace element, being a component of the enzymes xanthine oxidase, aldehyde oxidase, and nitrate reductase.	Molybdenum-98,Molybdenum 98
D002417	Cattle	Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.	Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D002467	Cell Nucleus	Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)	Cell Nuclei,Nuclei, Cell,Nucleus, Cell
D003300	Copper	A heavy metal trace element with the atomic symbol Cu, atomic number 29, and atomic weight 63.55.	Copper-63,Copper 63
D003594	Cytoplasmic Granules	Condensed areas of cellular material that may be bounded by a membrane.	Cytoplasmic Granule,Granule, Cytoplasmic,Granules, Cytoplasmic
D003600	Cytosol	Intracellular fluid from the cytoplasm after removal of ORGANELLES and other insoluble cytoplasmic components.	Cytosols