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A highly sensitive and novel colorimetric rolling circle amplification (RCA) immunoassay for detecting C-reactive protein (CRP) has been developed. In the assay, a CRP capture antibody was immobilized on magnetic beads and a CRP detection antibody was conjugated with single-stranded DNA (ssDNA) using N-[ε-maleimidocaproyloxy] sulfosuccinimide ester. Along with the addition of CRP, a "sandwich" structure was formed. Subsequently, the ssDNA was used as a primer to initiate the RCA reaction in the presence of the circular template, phi29 DNA polymerase and deoxynucleotide triphosphates. The RCA product obtained by magnetic separation, and long tandem repeated sequences mediated the aggregation of gold nanoparticles (AuNPs), which could be observed by the naked eye or quantified using absorption spectra with a detection limit of 30 fg mL(-1) and a linear response range from 10 ng mL(-1) to 1 pg mL(-1). This assay offers the advantages of isothermal conditions, low cost and label-free quantification that could be hopeful for ultrasensitive and robust visual protein detection.
Ping Wang, and
Bing Jin, and
Yasi Xing, and
Zule Cheng, and
Yuqing Ge, and
Honglian Zhang, and
Bing Hu, and
Hongju Mao, and
Qinghui Jin, and
Jianlong Zhao
April 2010,
Analytical chemistry,
Ping Wang, and
Bing Jin, and
Yasi Xing, and
Zule Cheng, and
Yuqing Ge, and
Honglian Zhang, and
Bing Hu, and
Hongju Mao, and
Qinghui Jin, and
Jianlong Zhao
July 2015,
The Analyst,
Ping Wang, and
Bing Jin, and
Yasi Xing, and
Zule Cheng, and
Yuqing Ge, and
Honglian Zhang, and
Bing Hu, and
Hongju Mao, and
Qinghui Jin, and
Jianlong Zhao
April 2013,
Biosensors & bioelectronics,
Ping Wang, and
Bing Jin, and
Yasi Xing, and
Zule Cheng, and
Yuqing Ge, and
Honglian Zhang, and
Bing Hu, and
Hongju Mao, and
Qinghui Jin, and
Jianlong Zhao
January 2008,
Electrophoresis,
Ping Wang, and
Bing Jin, and
Yasi Xing, and
Zule Cheng, and
Yuqing Ge, and
Honglian Zhang, and
Bing Hu, and
Hongju Mao, and
Qinghui Jin, and
Jianlong Zhao
January 2024,
Talanta,
Ping Wang, and
Bing Jin, and
Yasi Xing, and
Zule Cheng, and
Yuqing Ge, and
Honglian Zhang, and
Bing Hu, and
Hongju Mao, and
Qinghui Jin, and
Jianlong Zhao
May 2007,
Analytical chemistry,
Ping Wang, and
Bing Jin, and
Yasi Xing, and
Zule Cheng, and
Yuqing Ge, and
Honglian Zhang, and
Bing Hu, and
Hongju Mao, and
Qinghui Jin, and
Jianlong Zhao
June 2012,
Small (Weinheim an der Bergstrasse, Germany),
Ping Wang, and
Bing Jin, and
Yasi Xing, and
Zule Cheng, and
Yuqing Ge, and
Honglian Zhang, and
Bing Hu, and
Hongju Mao, and
Qinghui Jin, and
Jianlong Zhao
November 2010,
Small (Weinheim an der Bergstrasse, Germany),
Ping Wang, and
Bing Jin, and
Yasi Xing, and
Zule Cheng, and
Yuqing Ge, and
Honglian Zhang, and
Bing Hu, and
Hongju Mao, and
Qinghui Jin, and
Jianlong Zhao
February 2014,
ACS nano,
Ping Wang, and
Bing Jin, and
Yasi Xing, and
Zule Cheng, and
Yuqing Ge, and
Honglian Zhang, and
Bing Hu, and
Hongju Mao, and
Qinghui Jin, and
Jianlong Zhao
June 2021,
Luminescence : the journal of biological and chemical luminescence,
