[Establishment of C26 cell strain stably expressing folate receptor α]. 2015

Zheng Qiu, and Lijun Xing, and Xinxin Liu, and Mei Zhang, and Fang Zhang, and Min Wang
State Key Laboratory of Natural Medicines, Department of Molecular Biology, College of Life Sciences and Technology, China Pharmaceutical University, Nanjing 210009, China.

OBJECTIVE To establish a C26 cell strain stably expressing folate receptor α (FRα) for the subsequent study of FRα DNA vaccine. METHODS C26 cells were transfected with previously constructed recombinant eukaryotic expressing vector pcDNA3.1-FRα by LipofectamineTM2000. Afterwards the cells were subjected to G418 (500 mg/mL) selection to get G418 resistant cells. And then single cell cloning was performed to generate monoclonal cell strain. The gene and protein expression levels of FRα of the monoclonal cell strain were further analyzed by reverse transcription PCR (RT-PCR) and fluorescence microscopy. The cells were cultured for several generations, and the mRNA expression of FRα was analyzed by RT-PCR at different generations to determine whether the transfected gene was stable or not during cell passaging. RESULTS After transfection, G418 selection and single cell cloning, the monoclonal cell strain were established and proved to be able to express FRα mRNA and protein and keep the stability of FRα expression after several generations. CONCLUSIONS We have established the cell strain stably expressing FRα, which offer a tool to evaluate the effect of DNA vaccine based on FRα.

UI MeSH Term Description Entries
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D005821 Genetic Techniques Chromosomal, biochemical, intracellular, and other methods used in the study of genetics. Genetic Technic,Genetic Technics,Genetic Technique,Technic, Genetic,Technics, Genetic,Technique, Genetic,Techniques, Genetic
D005822 Genetic Vectors DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition. Cloning Vectors,Shuttle Vectors,Vectors, Genetic,Cloning Vector,Genetic Vector,Shuttle Vector,Vector, Cloning,Vector, Genetic,Vector, Shuttle,Vectors, Cloning,Vectors, Shuttle
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D014162 Transfection The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES. Transfections
D015870 Gene Expression The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION. Expression, Gene,Expressions, Gene,Gene Expressions
D045744 Cell Line, Tumor A cell line derived from cultured tumor cells. Tumor Cell Line,Cell Lines, Tumor,Line, Tumor Cell,Lines, Tumor Cell,Tumor Cell Lines
D051379 Mice The common name for the genus Mus. Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus
D058975 Folate Receptor 1 A subtype of GPI-anchored folate receptors that is expressed in tissues of epithelial origin. This protein is also identified as an ovarian-tumor-specific antigen. Folate Receptor alpha,Ovarian Tumor-Associated Antigen MOv18,Ovarian Tumor Associated Antigen MOv18,Receptor 1, Folate,Receptor alpha, Folate
D019444 Vaccines, DNA Recombinant DNA vectors encoding antigens administered for the prevention or treatment of disease. The host cells take up the DNA, express the antigen, and present it to the immune system in a manner similar to that which would occur during natural infection. This induces humoral and cellular immune responses against the encoded antigens. The vector is called naked DNA because there is no need for complex formulations or delivery agents; the plasmid is injected in saline or other buffers. DNA Vaccine,DNA Vaccines,Naked DNA Vaccine,Naked DNA Vaccines,Recombinant DNA Vaccine,Recombinant DNA Vaccines,Vaccines, Recombinant DNA,DNA Vaccine, Naked,DNA Vaccine, Recombinant,DNA Vaccines, Naked,DNA Vaccines, Recombinant,Vaccine, DNA,Vaccine, Naked DNA,Vaccine, Recombinant DNA,Vaccines, Naked DNA

Related Publications

Zheng Qiu, and Lijun Xing, and Xinxin Liu, and Mei Zhang, and Fang Zhang, and Min Wang
September 2015, Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology,
Zheng Qiu, and Lijun Xing, and Xinxin Liu, and Mei Zhang, and Fang Zhang, and Min Wang
February 1996, Japanese journal of medical science & biology,
Zheng Qiu, and Lijun Xing, and Xinxin Liu, and Mei Zhang, and Fang Zhang, and Min Wang
December 1996, Annals of the New York Academy of Sciences,
Zheng Qiu, and Lijun Xing, and Xinxin Liu, and Mei Zhang, and Fang Zhang, and Min Wang
March 2017, Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology,
Zheng Qiu, and Lijun Xing, and Xinxin Liu, and Mei Zhang, and Fang Zhang, and Min Wang
August 1995, Archives of biochemistry and biophysics,
Zheng Qiu, and Lijun Xing, and Xinxin Liu, and Mei Zhang, and Fang Zhang, and Min Wang
May 2006, Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology,
Zheng Qiu, and Lijun Xing, and Xinxin Liu, and Mei Zhang, and Fang Zhang, and Min Wang
July 2013, Nan fang yi ke da xue xue bao = Journal of Southern Medical University,
Zheng Qiu, and Lijun Xing, and Xinxin Liu, and Mei Zhang, and Fang Zhang, and Min Wang
September 2011, Sheng wu gong cheng xue bao = Chinese journal of biotechnology,
Zheng Qiu, and Lijun Xing, and Xinxin Liu, and Mei Zhang, and Fang Zhang, and Min Wang
August 2004, Zhongguo shi yan xue ye xue za zhi,
Zheng Qiu, and Lijun Xing, and Xinxin Liu, and Mei Zhang, and Fang Zhang, and Min Wang
January 2012, International journal of clinical and experimental medicine,
Copied contents to your clipboard!