Respiratory sinus arrhythmia (RSA) has emerged as an indicator of how well the body maintains homeostasis and flexibly responds to environmental demands. Previous research has shown that smoking has both acute and chronic effects on RSA in adults. More recent work has focused on adolescent smokers because the natural decrease in RSA over the lifespan might be hastened by smoking at an early age. The goal of the current study, then, was to examine the acute effects of smoking on RSA and mean heart rate (HR) in a group of adolescent smokers. Participants completed two experimental sessions separated by 6-10 weeks, during which resting electrocardiogram (EKG) data were collected before and after smoking or not smoking a single cigarette ad libitum. Results indicate that smoking significantly decreased resting RSA and increased mean HR. In addition, those who smoked their first cigarette earlier in life (i.e., before age 8 or 10) evidenced a greater decrease in RSA during their smoking session relative to those who tried smoking after age 10. Importantly, these findings are largely consistent with the adult literature and suggest that smoking has acute effects on both RSA and HR in adolescence.