[Determination of hexachlorocyclohexane and dichlorodiphenyltrichloroethen residues in honey by gas chromatography-mass spectrometry combined with dispersive liquid-liquid microextraction]. 2015

Dong Wang, and Chuanjin Hou, and Ercheng Zhao, and Chunhong Jia

A gas chromatography-mass spectrometry (GC-MS) method for the determination of hexachlorocyclohexane (BHC) and dichlorodiphenyltrichloroethane (DDT) residues in honey was developed using dispersive liquid-liquid microextraction (DLLME) as sample preparation method. The samples were extracted with chloroform, and concentrated into chloroform by vortex and centrifuging. The analytes were analyzed by GC-MS. The factors affecting the extraction efficiency were investigated, such as the type and volume of extraction and dispersive solvent, extraction time, etc. The matrix effects and method performance were evaluated at the same time. The results indicated that all the eight BHC and DDT pesticides displayed the enhancement of signals due to the matrix effects. The calibration curves were linear in the range of 2-500 microg/L and the correlation coefficients were 0.991-0.998 for the eight BHC and DDT pesticides. The enrichment factors (EF) were 74-96 for the eight pesticides. The recoveries of the eight BHC and DDT pesticides at three spiked levels of 20, 50 and 100 microg/kg were 61.0%-100.1%, while the RSDs were 2.2%-19.5%. The limits of quantification for the eight pesticides were 20 microg/kg, and the limits of detection were 1.0 ng. The developed method is easy, quick and with high performance. It is applicable for the determination of BHC and DDT residues in honey.

UI MeSH Term Description Entries
D008401 Gas Chromatography-Mass Spectrometry A microanalytical technique combining mass spectrometry and gas chromatography for the qualitative as well as quantitative determinations of compounds. Chromatography, Gas-Liquid-Mass Spectrometry,Chromatography, Gas-Mass Spectrometry,GCMS,Spectrometry, Mass-Gas Chromatography,Spectrum Analysis, Mass-Gas Chromatography,Gas-Liquid Chromatography-Mass Spectrometry,Mass Spectrometry-Gas Chromatography,Chromatography, Gas Liquid Mass Spectrometry,Chromatography, Gas Mass Spectrometry,Chromatography, Mass Spectrometry-Gas,Chromatography-Mass Spectrometry, Gas,Chromatography-Mass Spectrometry, Gas-Liquid,Gas Chromatography Mass Spectrometry,Gas Liquid Chromatography Mass Spectrometry,Mass Spectrometry Gas Chromatography,Spectrometries, Mass-Gas Chromatography,Spectrometry, Gas Chromatography-Mass,Spectrometry, Gas-Liquid Chromatography-Mass,Spectrometry, Mass Gas Chromatography,Spectrometry-Gas Chromatography, Mass,Spectrum Analysis, Mass Gas Chromatography
D010573 Pesticide Residues Pesticides or their breakdown products remaining in the environment following their normal use or accidental contamination. Pesticide Residue,Residue, Pesticide,Residues, Pesticide
D003634 DDT A polychlorinated pesticide that is resistant to destruction by light and oxidation. Its unusual stability has resulted in difficulties in residue removal from water, soil, and foodstuffs. This substance may reasonably be anticipated to be a carcinogen: Fourth Annual Report on Carcinogens (NTP-85-002, 1985). (From Merck Index, 11th ed) 1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane,4,4'-DDT,4,4'-Dichlorodiphenyltrichloroethane,Benzochloryl,Chlorophenothane,TbisC-ethane,p',p'-DDT,4,4' DDT,4,4' Dichlorodiphenyltrichloroethane,TbisC ethane
D006722 Honey A sweet viscous liquid food, produced in the honey sacs of various bees from nectar collected from flowers. The nectar is ripened into honey by inversion of its sucrose sugar into fructose and glucose. It is somewhat acidic and has mild antiseptic properties, being sometimes used in the treatment of burns and lacerations. Honeys
D001556 Hexachlorocyclohexane Any of several polyhalogenated organic compounds consisting of a six-carbon ring with one chlorine and one hydrogen attached to each carbon. Benzene Hexachloride,Lindane,gamma-Benzene Hexachloride,BHC Insecticide,Delitex,Epsilon-hexachlorocyclohexane,Eta-hexachlorocyclohexane,Gamma-666,Gammexane,Hexachlorane,Jacutin,Kwell,PMS-Lindane,Scabecid,Scabene,Scabisan,Tetocid,Zeta-hexachlorocyclohexane,gamma-HCH,gamma-Hexachlorocyclohexane,Epsilon hexachlorocyclohexane,Eta hexachlorocyclohexane,Gamma 666,Hexachloride, Benzene,Hexachloride, gamma-Benzene,Insecticide, BHC,PMS Lindane,Zeta hexachlorocyclohexane,gamma Benzene Hexachloride,gamma HCH,gamma Hexachlorocyclohexane
D059627 Liquid Phase Microextraction Miniaturized methods of liquid-liquid extraction. Liquid-Liquid Microextraction,Micro Liquid Phase Extraction,Micro Liquid-Liquid Extraction,Extraction, Micro Liquid-Liquid,Extractions, Micro Liquid-Liquid,Liquid Liquid Microextraction,Liquid Phase Microextractions,Liquid-Liquid Extraction, Micro,Liquid-Liquid Extractions, Micro,Liquid-Liquid Microextractions,Micro Liquid Liquid Extraction,Micro Liquid-Liquid Extractions,Microextraction, Liquid Phase,Microextraction, Liquid-Liquid,Microextractions, Liquid Phase,Microextractions, Liquid-Liquid,Phase Microextraction, Liquid,Phase Microextractions, Liquid

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