BACKGROUND Herbal products from Ayurveda were always in the forefront in providing leads to new drug discovery. Triphala, an ancient Ayurvedic herbal formulation comprises of equal portions of Amalaki, Bibhitaki and Haritaki and is used extensively for constipation, as an anti-inflammatory, analgesic, anti-arthritic, hypoglycemic and an anti-aging agent. OBJECTIVE To evaluate the effect of Triphala on dextran sulphate sodium induced colitis in rats. METHODS Present study carried out in total five groups (n = 6 in each group); first group served as normal, second group control, third group standard control and remaining two as test drug groups. Mesalzine was used as a standard drug for comparison. Two doses (150 mg/kg and 300 mg/kg) of Triphala were given as treatment for two separate groups of colitis rats for 7 days. C-reactive protein, superoxide dismutase, catalase, malondialdehyde levels were evaluated and histological study of the distal colon was conducted. RESULTS The colitis rats treated with higher dose of Triphala (300 mg/kg) exhibited normal parameters similar to normal control group animals, which is on par with standard drug mesalzine effect. CONCLUSIONS The results suggest that Triphala (300 mg/kg) has a considerable and reliable effect in reducing colitis in rats. This effect can be attributed to its antioxidant activity and well presence of flavonoids.