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To develop a hybrid artificial liver support system (ALSS), the authors constructed a rotating-disk type ALSS using hepatocytes entrapped within a calcium alginate hydrogel. This module was designed in imitation of the gas-liquid contactor that uses the same principle. Forty disks with film-shaped hydrogel were mounted to a horizontal rotating axis and were kept in contact with blood. The concentration of hepatocytes in the gel varied from 1.9 X 10(9) to 1.7 X 10(10) cells/L gel. Entrapping hepatocytes within hydrogel kept the hepatocytes viable, and results of in vitro experiments showed ammonium metabolism and urea synthesis. The results of ex vivo perfusion experiments using cats with acute hepatic insufficiency indicated that this module had the ability to replace liver function in vivo.
K Yanagi, and
K Ookawa, and
S Mizuno, and
N Ohshima
January 2004,
Artificial organs,
K Yanagi, and
K Ookawa, and
S Mizuno, and
N Ohshima
January 2002,
Surgery,
K Yanagi, and
K Ookawa, and
S Mizuno, and
N Ohshima
January 1987,
ASAIO transactions,
K Yanagi, and
K Ookawa, and
S Mizuno, and
N Ohshima
January 1988,
Annals of the New York Academy of Sciences,
K Yanagi, and
K Ookawa, and
S Mizuno, and
N Ohshima
February 1990,
Nihon Geka Gakkai zasshi,
K Yanagi, and
K Ookawa, and
S Mizuno, and
N Ohshima
January 1979,
Transactions - American Society for Artificial Internal Organs,
K Yanagi, and
K Ookawa, and
S Mizuno, and
N Ohshima
August 1992,
Artificial organs,
K Yanagi, and
K Ookawa, and
S Mizuno, and
N Ohshima
February 2005,
World journal of gastroenterology,
K Yanagi, and
K Ookawa, and
S Mizuno, and
N Ohshima
November 2001,
Annals of the New York Academy of Sciences,
K Yanagi, and
K Ookawa, and
S Mizuno, and
N Ohshima
May 2001,
Digestive diseases and sciences,
