CD47 blockade inhibits tumor progression human osteosarcoma in xenograft models. 2015

Ji-Feng Xu, and Xiao-Hong Pan, and Shui-Jun Zhang, and Chen Zhao, and Bin-Song Qiu, and Hai-Feng Gu, and Jian-Fei Hong, and Li Cao, and Yu Chen, and Bing Xia, and Qin Bi, and Ya-Ping Wang
Department of Orthopedics and Joint Surgery, Zhejiang Provincial People's Hospital, Hangzhou 310014, PR China.

Osteosarcoma is the most common bone tumors in children and adolescents. Despite intensive chemotherapy, patients with advanced disease still have a poor prognosis, illustrating the need for alternative therapies. In this study, we explored the use of antibodies that block CD47 with a tumor growth suppressive effect on osteosarcoma. We first found that up-regulation of CD47 mRNA levels in the tumorous tissues from eight patients with osteosarcoma when compared with that in adjacent non-tumorous tissues. Further western-blot (WB) and immunohistochemistry (IHC) demonstrated that CD47 protein level was highly expressed in osteosarcoma compared to normal osteoblastic cells and adjacent non-tumorous tissues. Osteosarcoma cancer stem cell markers staining shown that the majority of CD44+ cells expressed CD47 albeit with different percentages (ranging from 80% to 99%). Furthermore, high CD47 mRNA expression levels were associated with a decreased probability of progression-free and overall survival. In addition, blockade of CD47 by specific Abs suppresses the invasive ability of osteosarcoma tumor cells and further inhibits spontaneous pulmonary metastasis of KRIB osteosarcoma cells in vivo. Finally, CD47 blockade increases macrophage phagocytosis of osteosarcoma tumor cells.In conclusion, our findings demonstrate that CD47 is a critical regulator in the metastasis of osteosarcoma and suggest that targeted inhibition of this antigen by anti-CD47 may be a novel immunotherapeutic approach in the management of this tumor.

UI MeSH Term Description Entries
D008264 Macrophages The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.) Bone Marrow-Derived Macrophages,Monocyte-Derived Macrophages,Macrophage,Macrophages, Monocyte-Derived,Bone Marrow Derived Macrophages,Bone Marrow-Derived Macrophage,Macrophage, Bone Marrow-Derived,Macrophage, Monocyte-Derived,Macrophages, Bone Marrow-Derived,Macrophages, Monocyte Derived,Monocyte Derived Macrophages,Monocyte-Derived Macrophage
D008297 Male Males
D008809 Mice, Inbred C3H An inbred strain of mouse that is used as a general purpose strain in a wide variety of RESEARCH areas including CANCER; INFECTIOUS DISEASES; sensorineural, and cardiovascular biology research. Mice, C3H,Mouse, C3H,Mouse, Inbred C3H,C3H Mice,C3H Mice, Inbred,C3H Mouse,C3H Mouse, Inbred,Inbred C3H Mice,Inbred C3H Mouse
D009361 Neoplasm Invasiveness Ability of neoplasms to infiltrate and actively destroy surrounding tissue. Invasiveness, Neoplasm,Neoplasm Invasion,Invasion, Neoplasm
D009362 Neoplasm Metastasis The transfer of a neoplasm from one organ or part of the body to another remote from the primary site. Metastase,Metastasis,Metastases, Neoplasm,Metastasis, Neoplasm,Neoplasm Metastases,Metastases
D010587 Phagocytosis The engulfing and degradation of microorganisms; other cells that are dead, dying, or pathogenic; and foreign particles by phagocytic cells (PHAGOCYTES). Phagocytoses
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000906 Antibodies Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
D012333 RNA, Messenger RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm. Messenger RNA,Messenger RNA, Polyadenylated,Poly(A) Tail,Poly(A)+ RNA,Poly(A)+ mRNA,RNA, Messenger, Polyadenylated,RNA, Polyadenylated,mRNA,mRNA, Non-Polyadenylated,mRNA, Polyadenylated,Non-Polyadenylated mRNA,Poly(A) RNA,Polyadenylated mRNA,Non Polyadenylated mRNA,Polyadenylated Messenger RNA,Polyadenylated RNA,RNA, Polyadenylated Messenger,mRNA, Non Polyadenylated

Related Publications

Ji-Feng Xu, and Xiao-Hong Pan, and Shui-Jun Zhang, and Chen Zhao, and Bin-Song Qiu, and Hai-Feng Gu, and Jian-Fei Hong, and Li Cao, and Yu Chen, and Bing Xia, and Qin Bi, and Ya-Ping Wang
January 2013, Molecular imaging,
Ji-Feng Xu, and Xiao-Hong Pan, and Shui-Jun Zhang, and Chen Zhao, and Bin-Song Qiu, and Hai-Feng Gu, and Jian-Fei Hong, and Li Cao, and Yu Chen, and Bing Xia, and Qin Bi, and Ya-Ping Wang
December 2013, Oncotarget,
Ji-Feng Xu, and Xiao-Hong Pan, and Shui-Jun Zhang, and Chen Zhao, and Bin-Song Qiu, and Hai-Feng Gu, and Jian-Fei Hong, and Li Cao, and Yu Chen, and Bing Xia, and Qin Bi, and Ya-Ping Wang
January 2018, Journal of immunology research,
Ji-Feng Xu, and Xiao-Hong Pan, and Shui-Jun Zhang, and Chen Zhao, and Bin-Song Qiu, and Hai-Feng Gu, and Jian-Fei Hong, and Li Cao, and Yu Chen, and Bing Xia, and Qin Bi, and Ya-Ping Wang
June 2015, Carbohydrate polymers,
Ji-Feng Xu, and Xiao-Hong Pan, and Shui-Jun Zhang, and Chen Zhao, and Bin-Song Qiu, and Hai-Feng Gu, and Jian-Fei Hong, and Li Cao, and Yu Chen, and Bing Xia, and Qin Bi, and Ya-Ping Wang
May 2017, Experimental and therapeutic medicine,
Ji-Feng Xu, and Xiao-Hong Pan, and Shui-Jun Zhang, and Chen Zhao, and Bin-Song Qiu, and Hai-Feng Gu, and Jian-Fei Hong, and Li Cao, and Yu Chen, and Bing Xia, and Qin Bi, and Ya-Ping Wang
May 2015, Cancer letters,
Ji-Feng Xu, and Xiao-Hong Pan, and Shui-Jun Zhang, and Chen Zhao, and Bin-Song Qiu, and Hai-Feng Gu, and Jian-Fei Hong, and Li Cao, and Yu Chen, and Bing Xia, and Qin Bi, and Ya-Ping Wang
May 2012, International journal of oncology,
Ji-Feng Xu, and Xiao-Hong Pan, and Shui-Jun Zhang, and Chen Zhao, and Bin-Song Qiu, and Hai-Feng Gu, and Jian-Fei Hong, and Li Cao, and Yu Chen, and Bing Xia, and Qin Bi, and Ya-Ping Wang
November 2012, Journal of agricultural and food chemistry,
Ji-Feng Xu, and Xiao-Hong Pan, and Shui-Jun Zhang, and Chen Zhao, and Bin-Song Qiu, and Hai-Feng Gu, and Jian-Fei Hong, and Li Cao, and Yu Chen, and Bing Xia, and Qin Bi, and Ya-Ping Wang
December 2020, International journal of molecular sciences,
Ji-Feng Xu, and Xiao-Hong Pan, and Shui-Jun Zhang, and Chen Zhao, and Bin-Song Qiu, and Hai-Feng Gu, and Jian-Fei Hong, and Li Cao, and Yu Chen, and Bing Xia, and Qin Bi, and Ya-Ping Wang
May 2007, Oncology reports,
Copied contents to your clipboard!