UV induced ubiquitination of the yeast Rad4-Rad23 complex promotes survival by regulating cellular dNTP pools. 2015

Zheng Zhou, and Neil Humphryes, and Patrick van Eijk, and Raymond Waters, and Shirong Yu, and Rolf Kraehenbuehl, and Edgar Hartsuiker, and Simon H Reed
Institute of Cancer & Genetics, School of Medicine, Cardiff University, Heath Park, Cardiff, CF14 4XN, UK College of Biology, Hunan University, Changsha 410082, China.

Regulating gene expression programmes is a central facet of the DNA damage response. The Dun1 kinase protein controls expression of many DNA damage induced genes, including the ribonucleotide reductase genes, which regulate cellular dNTP pools. Using a combination of gene expression profiling and chromatin immunoprecipitation, we demonstrate that in the absence of DNA damage the yeast Rad4-Rad23 nucleotide excision repair complex binds to the promoters of certain DNA damage response genes including DUN1, inhibiting their expression. UV radiation promotes the loss of occupancy of the Rad4-Rad23 complex from the regulatory regions of these genes, enabling their induction and thereby controlling the production of dNTPs. We demonstrate that this regulatory mechanism, which is dependent on the ubiquitination of Rad4 by the GG-NER E3 ligase, promotes UV survival in yeast cells. These results support an unanticipated regulatory mechanism that integrates ubiquitination of NER DNA repair factors with the regulation of the transcriptional response controlling dNTP production and cellular survival after UV damage.

UI MeSH Term Description Entries
D011401 Promoter Regions, Genetic DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes. rRNA Promoter,Early Promoters, Genetic,Late Promoters, Genetic,Middle Promoters, Genetic,Promoter Regions,Promoter, Genetic,Promotor Regions,Promotor, Genetic,Pseudopromoter, Genetic,Early Promoter, Genetic,Genetic Late Promoter,Genetic Middle Promoters,Genetic Promoter,Genetic Promoter Region,Genetic Promoter Regions,Genetic Promoters,Genetic Promotor,Genetic Promotors,Genetic Pseudopromoter,Genetic Pseudopromoters,Late Promoter, Genetic,Middle Promoter, Genetic,Promoter Region,Promoter Region, Genetic,Promoter, Genetic Early,Promoter, rRNA,Promoters, Genetic,Promoters, Genetic Middle,Promoters, rRNA,Promotor Region,Promotors, Genetic,Pseudopromoters, Genetic,Region, Genetic Promoter,Region, Promoter,Region, Promotor,Regions, Genetic Promoter,Regions, Promoter,Regions, Promotor,rRNA Promoters
D003854 Deoxyribonucleotides A purine or pyrimidine base bonded to a DEOXYRIBOSE containing a bond to a phosphate group. Deoxyribonucleotide
D004249 DNA Damage Injuries to DNA that introduce deviations from its normal, intact structure and which may, if left unrepaired, result in a MUTATION or a block of DNA REPLICATION. These deviations may be caused by physical or chemical agents and occur by natural or unnatural, introduced circumstances. They include the introduction of illegitimate bases during replication or by deamination or other modification of bases; the loss of a base from the DNA backbone leaving an abasic site; single-strand breaks; double strand breaks; and intrastrand (PYRIMIDINE DIMERS) or interstrand crosslinking. Damage can often be repaired (DNA REPAIR). If the damage is extensive, it can induce APOPTOSIS. DNA Injury,DNA Lesion,DNA Lesions,Genotoxic Stress,Stress, Genotoxic,Injury, DNA,DNA Injuries
D004260 DNA Repair The removal of DNA LESIONS and/or restoration of intact DNA strands without BASE PAIR MISMATCHES, intrastrand or interstrand crosslinks, or discontinuities in the DNA sugar-phosphate backbones. DNA Damage Response
D004268 DNA-Binding Proteins Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases. DNA Helix Destabilizing Proteins,DNA-Binding Protein,Single-Stranded DNA Binding Proteins,DNA Binding Protein,DNA Single-Stranded Binding Protein,SS DNA BP,Single-Stranded DNA-Binding Protein,Binding Protein, DNA,DNA Binding Proteins,DNA Single Stranded Binding Protein,DNA-Binding Protein, Single-Stranded,Protein, DNA-Binding,Single Stranded DNA Binding Protein,Single Stranded DNA Binding Proteins
D005656 Fungal Proteins Proteins found in any species of fungus. Fungal Gene Products,Fungal Gene Proteins,Fungal Peptides,Gene Products, Fungal,Yeast Proteins,Gene Proteins, Fungal,Peptides, Fungal,Proteins, Fungal
D014158 Transcription, Genetic The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION. Genetic Transcription
D014466 Ultraviolet Rays That portion of the electromagnetic spectrum immediately below the visible range and extending into the x-ray frequencies. The longer wavelengths (near-UV or biotic or vital rays) are necessary for the endogenous synthesis of vitamin D and are also called antirachitic rays; the shorter, ionizing wavelengths (far-UV or abiotic or extravital rays) are viricidal, bactericidal, mutagenic, and carcinogenic and are used as disinfectants. Actinic Rays,Black Light, Ultraviolet,UV Light,UV Radiation,Ultra-Violet Rays,Ultraviolet Light,Ultraviolet Radiation,Actinic Ray,Light, UV,Light, Ultraviolet,Radiation, UV,Radiation, Ultraviolet,Ray, Actinic,Ray, Ultra-Violet,Ray, Ultraviolet,Ultra Violet Rays,Ultra-Violet Ray,Ultraviolet Black Light,Ultraviolet Black Lights,Ultraviolet Radiations,Ultraviolet Ray
D015003 Yeasts A general term for single-celled rounded fungi that reproduce by budding. Brewers' and bakers' yeasts are SACCHAROMYCES CEREVISIAE; therapeutic dried yeast is YEAST, DRIED. Yeast
D015966 Gene Expression Regulation, Fungal Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in fungi. Fungal Gene Expression Regulation,Regulation of Gene Expression, Fungal,Regulation, Gene Expression, Fungal

Related Publications

Zheng Zhou, and Neil Humphryes, and Patrick van Eijk, and Raymond Waters, and Shirong Yu, and Rolf Kraehenbuehl, and Edgar Hartsuiker, and Simon H Reed
December 1998, The Journal of biological chemistry,
Zheng Zhou, and Neil Humphryes, and Patrick van Eijk, and Raymond Waters, and Shirong Yu, and Rolf Kraehenbuehl, and Edgar Hartsuiker, and Simon H Reed
April 2022, Life science alliance,
Zheng Zhou, and Neil Humphryes, and Patrick van Eijk, and Raymond Waters, and Shirong Yu, and Rolf Kraehenbuehl, and Edgar Hartsuiker, and Simon H Reed
January 2004, Nucleic acids research,
Zheng Zhou, and Neil Humphryes, and Patrick van Eijk, and Raymond Waters, and Shirong Yu, and Rolf Kraehenbuehl, and Edgar Hartsuiker, and Simon H Reed
March 1998, The Plant journal : for cell and molecular biology,
Zheng Zhou, and Neil Humphryes, and Patrick van Eijk, and Raymond Waters, and Shirong Yu, and Rolf Kraehenbuehl, and Edgar Hartsuiker, and Simon H Reed
October 2002, DNA repair,
Zheng Zhou, and Neil Humphryes, and Patrick van Eijk, and Raymond Waters, and Shirong Yu, and Rolf Kraehenbuehl, and Edgar Hartsuiker, and Simon H Reed
June 2019, The Journal of biological chemistry,
Zheng Zhou, and Neil Humphryes, and Patrick van Eijk, and Raymond Waters, and Shirong Yu, and Rolf Kraehenbuehl, and Edgar Hartsuiker, and Simon H Reed
November 1998, The Journal of biological chemistry,
Zheng Zhou, and Neil Humphryes, and Patrick van Eijk, and Raymond Waters, and Shirong Yu, and Rolf Kraehenbuehl, and Edgar Hartsuiker, and Simon H Reed
January 1994, Advances in experimental medicine and biology,
Zheng Zhou, and Neil Humphryes, and Patrick van Eijk, and Raymond Waters, and Shirong Yu, and Rolf Kraehenbuehl, and Edgar Hartsuiker, and Simon H Reed
April 1995, The Journal of biological chemistry,
Zheng Zhou, and Neil Humphryes, and Patrick van Eijk, and Raymond Waters, and Shirong Yu, and Rolf Kraehenbuehl, and Edgar Hartsuiker, and Simon H Reed
May 2017, Proceedings of the National Academy of Sciences of the United States of America,
Copied contents to your clipboard!