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Insights into unknown foreign ligand in copper nitrite reductase. 2015

Yohta Fukuda, and Ka Man Tse, and Yuji Kado, and Eiichi Mizohata, and Hiroyoshi Matsumura, and Tsuyoshi Inoue
Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.

Bifunctional copper nitrite reductase (CuNIR) catalyzes nitrite reduction to nitric oxide and dioxygen reduction to hydrogen peroxide. In contrast to the well-researched nitrite reduction mechanism, the oxygen reduction mechanism in CuNIR has been totally unknown, because mononuclear copper-oxygen complexes decompose so readily that their visualization has been challenging. Here, we provide spectroscopic evidence that a foreign ligand binds to the catalytic copper (T2Cu) site of CuNIR, and determine CuNIR structures displaying a diatomic molecule on T2Cu. This unknown ligand can be interpreted as dioxygen and may provide insights into the oxygen reduction mechanism of CuNIR.

UI MeSH Term Description Entries
D008024 Ligands A molecule that binds to another molecule, used especially to refer to a small molecule that binds specifically to a larger molecule, e.g., an antigen binding to an antibody, a hormone or neurotransmitter binding to a receptor, or a substrate or allosteric effector binding to an enzyme. Ligands are also molecules that donate or accept a pair of electrons to form a coordinate covalent bond with the central metal atom of a coordination complex. (From Dorland, 27th ed) Ligand
D009572 Nitrite Reductases A group of enzymes that oxidize diverse nitrogenous substances to yield nitrite. (Enzyme Nomenclature, 1992) EC 1. Nitrite Reductase,Reductase, Nitrite,Reductases, Nitrite
D011487 Protein Conformation The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain). Conformation, Protein,Conformations, Protein,Protein Conformations
D013056 Spectrophotometry, Ultraviolet Determination of the spectra of ultraviolet absorption by specific molecules in gases or liquids, for example Cl2, SO2, NO2, CS2, ozone, mercury vapor, and various unsaturated compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Ultraviolet Spectrophotometry
D018360 Crystallography, X-Ray The study of crystal structure using X-RAY DIFFRACTION techniques. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) X-Ray Crystallography,Crystallography, X Ray,Crystallography, Xray,X Ray Crystallography,Xray Crystallography,Crystallographies, X Ray,X Ray Crystallographies

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