Biomaterial Database

In vivo chemotaxis evoked by Actinobacillus actinomycetemcomitans and Haemophilus aphrophilus. 1989

A Holm, and S Kalfas, and S E Holm

The chemotaxis-evoking capacity of 5 Actinobacillus actinomycetemcomitans and 5 Haemophilus aphrophilus strains were studied in a tissue cage model in rabbits. A significant increase of the total number of polymorphonuclear leukocytes was induced in the tissue cage fluid by both viable and killed bacteria, reaching a maximum after 12-24 h. In parallel, the proportion of viable/non viable leukocytes increased. The leukocyte counts declined during the following 24-48 h in all chambers except in those inoculated with viable cells of H. aphrophilus. The H. aphrophilus strains survived the 72 h experiment while A. actinomycetemcomitans decreased to undetectable levels within 24-72 h. Lactate dehydrogenase and lysozyme activities in cage fluid increased in all but the uninoculated chambers. Viable bacteria induced higher activities of the enzymes than killed ones. It is concluded that both species of bacteria exhibit similar chemotaxis evoking properties. A strain dependent ability to induce release of leukocyte-associated enzymes exists.

UI	MeSH Term	Description	Entries
D009504	Neutrophils	Granular leukocytes having a nucleus with three to five lobes connected by slender threads of chromatin, and cytoplasm containing fine inconspicuous granules and stainable by neutral dyes.	LE Cells,Leukocytes, Polymorphonuclear,Polymorphonuclear Leukocytes,Polymorphonuclear Neutrophils,Neutrophil Band Cells,Band Cell, Neutrophil,Cell, LE,LE Cell,Leukocyte, Polymorphonuclear,Neutrophil,Neutrophil Band Cell,Neutrophil, Polymorphonuclear,Polymorphonuclear Leukocyte,Polymorphonuclear Neutrophil
D011817	Rabbits	A burrowing plant-eating mammal with hind limbs that are longer than its fore limbs. It belongs to the family Leporidae of the order Lagomorpha, and in contrast to hares, possesses 22 instead of 24 pairs of chromosomes.	Belgian Hare,New Zealand Rabbit,New Zealand Rabbits,New Zealand White Rabbit,Rabbit,Rabbit, Domestic,Chinchilla Rabbits,NZW Rabbits,New Zealand White Rabbits,Oryctolagus cuniculus,Chinchilla Rabbit,Domestic Rabbit,Domestic Rabbits,Hare, Belgian,NZW Rabbit,Rabbit, Chinchilla,Rabbit, NZW,Rabbit, New Zealand,Rabbits, Chinchilla,Rabbits, Domestic,Rabbits, NZW,Rabbits, New Zealand,Zealand Rabbit, New,Zealand Rabbits, New,cuniculus, Oryctolagus
D002634	Chemotaxis, Leukocyte	The movement of leukocytes in response to a chemical concentration gradient or to products formed in an immunologic reaction.	Leukotaxis,Leukocyte Chemotaxis
D006190	Haemophilus	A genus of PASTEURELLACEAE that consists of several species occurring in animals and humans. Its organisms are described as gram-negative, facultatively anaerobic, coccobacillus or rod-shaped, and nonmotile.	Hemophilus
D000188	Actinobacillus	A genus of PASTEURELLACEAE described as gram-negative, nonsporeforming, nonmotile, facultative anaerobes. Most members are found both as pathogens and commensal organisms in the respiratory, alimentary, and genital tracts of animals.
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D015194	Diffusion Chambers, Culture	Devices used in a technique by which cells or tissues are grown in vitro or, by implantation, in vivo within chambers permeable to diffusion of solutes across the chamber walls. The chambers are used for studies of drug effects, osmotic responses, cytogenic and immunologic phenomena, metabolism, etc., and include tissue cages.	Culture Diffusion Chambers,Semi-Permeable Chambers,Tissue Cages,Semipermeable Chambers,Cage, Tissue,Cages, Tissue,Chamber, Culture Diffusion,Chamber, Semi-Permeable,Chamber, Semipermeable,Chambers, Culture Diffusion,Chambers, Semi-Permeable,Chambers, Semipermeable,Culture Diffusion Chamber,Diffusion Chamber, Culture,Semi Permeable Chambers,Semi-Permeable Chamber,Semipermeable Chamber,Tissue Cage