BACKGROUND Chronic ankle instability is a frequent and serious consequence of lateral ankle sprains. The contribution of perceived instability and potential for mechanical laxity to contribute to the overall deficit in dynamic postural stability is unclear. The purpose was to determine if those with mechanical laxity demonstrated significant differences in dynamic postural stability compared to controls, copers and those with perceived instability. METHODS Of 93 participants, 83 recreationally active individuals were divided into 4 groups: controls, copers, those with perceived instability, and those with both perceived instability and mechanical laxity. Injury history and the Cumberland Ankle Instability Tool were collected, and an instrumented arthrometer was applied. Participants completed a single limb jump landing, balancing upon completion. Ground reaction force data were collected, scaled to body mass, and the Dynamic Postural Stability Indices were calculated for anterior-posterior, medial-lateral, vertical and composite. One-way ANOVAs with Tukey post-hoc tests (α<0.05) were conducted on each of the stability indices among the four groups. RESULTS The mechanically lax group had significantly greater mean (standard deviation) medial-lateral stability index scores 0.57 (0.62) than the coper group 0.24 (0.20; P=0.02) and significantly greater composite index scores 0.73 (0.57) than the perceived instability 0.49 (0.09) and coper groups 0.47 (0.12 P=0.05). No other indices were significantly different among groups. CONCLUSIONS Individuals with perceived instability and mechanical laxity exhibited dynamic postural deficits compared to copers and those with perceived instability alone. Mechanical laxity may contribute to the deficits in dynamic postural stability.