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A method for determination of amino acid composition of proteins separated by SDS-polyacrylamide gel electrophoresis and transferred to polyvinylidene difluoride (PVDF) membranes is described. A single blotted band containing 50 to 200 pmoles of protein was cut out and submitted to acid hydrolysis with HCl followed by derivatization with phenylisothiocyanate. The amino acid derivatives were separated by reverse phase high-performance liquid chromatography. Bovine serum albumin, lysozyme, myoglobin, ovalbumin, soybean trypsin inhibitor and carbonic anhydrase were analyzed; the results revealed a good correspondence with reported values. This can be considered an analytical method to determine the amino acid composition of samples from microquantities of protein mixtures, particularly in those cases in which SDS-polyacrylamide gel electrophoresis is the most suitable separation system.
A Santucci, and
L Lozzi, and
L Bracci, and
S Petreni, and
M Rustici, and
P Soldani, and
P Neri
July 1987,
The Journal of biological chemistry,
A Santucci, and
L Lozzi, and
L Bracci, and
S Petreni, and
M Rustici, and
P Soldani, and
P Neri
May 1990,
Analytical biochemistry,
A Santucci, and
L Lozzi, and
L Bracci, and
S Petreni, and
M Rustici, and
P Soldani, and
P Neri
August 1989,
Analytical biochemistry,
A Santucci, and
L Lozzi, and
L Bracci, and
S Petreni, and
M Rustici, and
P Soldani, and
P Neri
May 1989,
Analytical biochemistry,
A Santucci, and
L Lozzi, and
L Bracci, and
S Petreni, and
M Rustici, and
P Soldani, and
P Neri
March 1992,
Electrophoresis,
A Santucci, and
L Lozzi, and
L Bracci, and
S Petreni, and
M Rustici, and
P Soldani, and
P Neri
August 1989,
Journal of chromatography,
A Santucci, and
L Lozzi, and
L Bracci, and
S Petreni, and
M Rustici, and
P Soldani, and
P Neri
September 1999,
Journal of biomolecular techniques : JBT,
A Santucci, and
L Lozzi, and
L Bracci, and
S Petreni, and
M Rustici, and
P Soldani, and
P Neri
March 2001,
Electrophoresis,
A Santucci, and
L Lozzi, and
L Bracci, and
S Petreni, and
M Rustici, and
P Soldani, and
P Neri
January 1992,
Electrophoresis,
A Santucci, and
L Lozzi, and
L Bracci, and
S Petreni, and
M Rustici, and
P Soldani, and
P Neri
November 1990,
Analytical biochemistry,
